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Team:Freiburg/Notebook/20 June
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| - | Incubation of cells at 30 °C for 3 hours. Half of these cells were then heated up to 42°C. | + | 5 tubes with 2ml LB, 1 microl Chloramphenicol and 100 microl cells with the lysis cassette. Incubation of cells at 30 °C for 3 hours. Half of these cells were then heated up to 42°C. Miniprep was followed. |
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| + | ==Digestion of PR1-PR9== | ||
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| + | '''Investigators: Julia, Theo''' | ||
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| + | Digestion of PR1, PR2, PR3, PR4, PR5, PR6, PR7, PR8 and PR9. | ||
| + | Enzyme 1: XhoI | ||
| + | Enzyme 2: PstI | ||
| + | Samples were then loaded onto a gel. | ||
Revision as of 08:38, 13 July 2011
15. Labday
Lysis cassette test
Investigators: Theo
Comments: cells were used with the lysis cassette transformed on Saturday
5 tubes with 2ml LB, 1 microl Chloramphenicol and 100 microl cells with the lysis cassette. Incubation of cells at 30 °C for 3 hours. Half of these cells were then heated up to 42°C. Miniprep was followed.
Digestion of PR1-PR9
Investigators: Julia, Theo
Digestion of PR1, PR2, PR3, PR4, PR5, PR6, PR7, PR8 and PR9. Enzyme 1: XhoI Enzyme 2: PstI Samples were then loaded onto a gel.
