Team:LMU-Munich/Project

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== '''Overall project''' ==
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==<font color="#9933CC">'''Overall project'''</font>==
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Your abstract
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Metals and especially heavy metals are highly prescribed in concentrations in the drinking water ordinance. Qualifying and quantifying these by standard chemical methods is costly and complicated.
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Bacteria sense metals in their surrounding in order to change their expression profile or react in order to adapt and accomodate to their environment.
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Using these sensors from (mostly) bacteria we create biosensors by linking them to the expression of a reporter (e.g. green glowing by the green fluorescent protein GFP). To not only qualify but also to quantify the metals, it is also necessary to measure the output by given input (metal concentration) for each of these biosensors. Afterwards one can determine the metal concentration by measuring the output.
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The quantification needs heavy high-tech machinery ... something not always given ... especially in free field. So a qualification of metals with a easy-to-see output is also needed.
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In the end our team hopes to have not only a set of metallsensors for precise quantification of a group of (heavy) metalls, but also an outdoor kit for qualifying metalls in more remote areas. With these it might be more easy and cheaper to determine the content of metals in our drinking water.
== Project Details==
== Project Details==

Revision as of 07:53, 13 July 2011


This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page. PLEASE keep all of your pages within your teams namespace.


You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.

Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)

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Contents

Overall project

Metals and especially heavy metals are highly prescribed in concentrations in the drinking water ordinance. Qualifying and quantifying these by standard chemical methods is costly and complicated.

Bacteria sense metals in their surrounding in order to change their expression profile or react in order to adapt and accomodate to their environment.

Using these sensors from (mostly) bacteria we create biosensors by linking them to the expression of a reporter (e.g. green glowing by the green fluorescent protein GFP). To not only qualify but also to quantify the metals, it is also necessary to measure the output by given input (metal concentration) for each of these biosensors. Afterwards one can determine the metal concentration by measuring the output.

The quantification needs heavy high-tech machinery ... something not always given ... especially in free field. So a qualification of metals with a easy-to-see output is also needed.

In the end our team hopes to have not only a set of metallsensors for precise quantification of a group of (heavy) metalls, but also an outdoor kit for qualifying metalls in more remote areas. With these it might be more easy and cheaper to determine the content of metals in our drinking water.

Project Details

Part 2

The Experiments

Part 3

Results