Team:KAIST-Korea/Proposal2

(Difference between revisions)

Revision as of 12:46, 25 April 2011

Proposal 2

Necessity

Even in the absence of an inducer, genes introduced via a vector are translated into proteins. This is termed leaky expression.
These proteins draw energy reserved for cell's metabolism.
Hence, life times of cells decrease.

Approach

Attach a sigma factor to a zinc finger and anchor the fusion protein in the T7 promoter region.
RNA polymerase bound for the promoter are coaxed to bound to the fusion protein.
Less RNA polymerase binds to the promoter, therefore, and hence leaky expression decreases.

Thank you!

@@ Line 20: / Line 20: @@
 <center><span style =font-size:20px><font face="Maiandra GD"> Necessity </font></span></center>
 <br>
-# &nbsp;&nbsp;&nbsp;Even in the absence of an inducer, genes introduced via a vector are translated into proteins. This is termed leaky expression.
+# Even in the absence of an inducer, genes introduced via a vector are translated into proteins. This is termed leaky expression.
-# &nbsp;&nbsp;&nbsp;These proteins draw energy reserved for cell's metabolism.
+# These proteins draw energy reserved for cell's metabolism.
-# &nbsp;&nbsp;&nbsp;Hence, life times of cells decrease.
+# Hence, life times of cells decrease.
 <br>
@@ Line 29: / Line 29: @@
 <br>
 <br>
-# &nbsp;&nbsp;&nbsp;Attach a sigma factor to a zinc finger and anchor the fusion protein in the T7 promoter region.
+# Attach a sigma factor to a zinc finger and anchor the fusion protein in the T7 promoter region.
-# &nbsp;&nbsp;&nbsp;RNA polymerase bound for the promoter are coaxed to bound to the fusion protein.
+# RNA polymerase bound for the promoter are coaxed to bound to the fusion protein.
-# &nbsp;&nbsp;&nbsp;Less RNA polymerase binds to the promoter, therefore, and hence leaky expression decreases.
+# Less RNA polymerase binds to the promoter, therefore, and hence leaky expression decreases.
 <br>
 <br>