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Team:ETH Zurich/Project
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| - | -Our biological system acts like a cigarette smoke detector. | + | -Our biological system acts like a cigarette smoke detector which will produce flashes of light as a response of the concentration of the smoke in the air. |
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-To build our system we use E.Coli bacteria. | -To build our system we use E.Coli bacteria. | ||
- We modify our cells so that they can detect Acetaldehyde, which is a substance found in the cigarete smoke. Upon binding of Acetaldehyde to AlcR, AlcR acts as an inhibitor for a gene transcription. This is where our genetically engineered circuit? / pathway? starts. With a number of inhibitors and activators, we crated our circuit such that it gives rise to a GFP product once a certain concentration of Acetaldehyde is detected from the air. Thus, our circuit acts as a band-pass-filter for Acetaldehyde concentration. | - We modify our cells so that they can detect Acetaldehyde, which is a substance found in the cigarete smoke. Upon binding of Acetaldehyde to AlcR, AlcR acts as an inhibitor for a gene transcription. This is where our genetically engineered circuit? / pathway? starts. With a number of inhibitors and activators, we crated our circuit such that it gives rise to a GFP product once a certain concentration of Acetaldehyde is detected from the air. Thus, our circuit acts as a band-pass-filter for Acetaldehyde concentration. | ||
Revision as of 12:47, 12 July 2011
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Overall project
Project description -Our biological system acts like a cigarette smoke detector which will produce flashes of light as a response of the concentration of the smoke in the air.
-To build our system we use E.Coli bacteria. - We modify our cells so that they can detect Acetaldehyde, which is a substance found in the cigarete smoke. Upon binding of Acetaldehyde to AlcR, AlcR acts as an inhibitor for a gene transcription. This is where our genetically engineered circuit? / pathway? starts. With a number of inhibitors and activators, we crated our circuit such that it gives rise to a GFP product once a certain concentration of Acetaldehyde is detected from the air. Thus, our circuit acts as a band-pass-filter for Acetaldehyde concentration.
We will place our genetically engineered cells in a microfluidics channel and will supply them with a flow of Acetaldehyde. We will also design our cells to degrade Acetaldehyde, so that a concentration gradient of Acetaldehyde is created throughout the channel.
