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Team:Freiburg/Notebook/12 July
From 2011.igem.org
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SophieCramer (Talk | contribs) (→Sequence Analysis) |
SophieCramer (Talk | contribs) (→Sequence Analysis) |
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==Lysis cassette and heat induced promoter== | ==Lysis cassette and heat induced promoter== | ||
===Lysis cassette=== | ===Lysis cassette=== | ||
| - | S15b (Lysis cassette K124014) is named K124017 in the registry | + | S15b: (Lysis cassette K124014) is named K124017 in the registry |
Part without RBS at the beginning | Part without RBS at the beginning | ||
Part without antiholin | Part without antiholin | ||
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==Green light receptor== | ==Green light receptor== | ||
===CcaR=== | ===CcaR=== | ||
| - | S17 (CcaR with Prefix and RBS) is ok. Check RBS. | + | S17: (CcaR with Prefix and RBS) is ok. Check RBS. |
| - | S18 seems to be ok too. | + | S18: seems to be ok too. |
===pycA+terminator=== | ===pycA+terminator=== | ||
| Line 18: | Line 18: | ||
===cph8=== | ===cph8=== | ||
| - | S29: Sequence without part | + | S29: Sequence without part. Has to be done again. |
===LOV3=== | ===LOV3=== | ||
| - | S35 | + | S35: is K322999 |
Revision as of 12:02, 12 July 2011
Contents |
Sequence Analysis
Lysis cassette and heat induced promoter
Lysis cassette
S15b: (Lysis cassette K124014) is named K124017 in the registry Part without RBS at the beginning Part without antiholin the sequece is also mutated leading to wrong amino acids if combined with RBS there would be too many bases (8) between RBS and coding sequence part is likely to be not effective
Green light receptor
CcaR
S17: (CcaR with Prefix and RBS) is ok. Check RBS. S18: seems to be ok too.
pycA+terminator
S24: prefix and suffix not complete
cph8
S29: Sequence without part. Has to be done again.
LOV3
S35: is K322999
