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| - | {{:Team:UNAM-Genomics Mexico/Templates/borrando}} | + | {{:Team:UNAM-Genomics Mexico/Templates/Notebook| content= |
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| - | <html xmlns="http://www.w3.org/1999/xhtml">
| + | ==Abstract== |
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| + | Molecular hydrogen is an alternative energy carrier to electricity and hydrocarbon derived fuels. Among the natural systems that produce hydrogen, the most efficient ones are those that produce it through reactions catalyzed by enzymes with iron-sulfur clusters which require hypoxic microenvironments to work. The bacterium ''Rhizobium etli'', during its symbiotic relationship with the common bean ''Phaseolus vulgaris'', can transform nitrogen gas (N<sub>2</sub>) into ammonia (NH<sub>3</sub>) in a process called nitrogen fixation. The bean plant benefits from the produced nitrogen containing compounds. In exchange it provides the bacteria with carbon sources and a protected niche inside its root, where ''Rhizobium etli'' reaches a hypoxic state. We will take advantage of this hypoxic state to produce hydrogen in ''Rhizobium etli'' while maintaining nitrogen fixation. |
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| - | @NAME Project page for the UNAM-Genomics_Mexico team wiki.
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| - | <title>UNAM-Genomics_Mexico</title>
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| + | ''Rhizobium etli'' is not commonly used in biological engineering. Thus we will both develop new tools and characterize parts already existing in the registry to work in a standardized way with ''Rhizobium etli''. Our tools include plasmids, regulatory upstream regions and transcriptional terminators. The hydrogen-production pathway includes elements from the bacteria ''Clostridium acetobutylicum'' and ''Desulfovibrio africanus'', and the algae ''Chlamydomonas reinhardtii''. The two main goals of our project are to make ''Rhizobium etli'' a powerful agent in environmental protection by nitrifying soils and producing hydrogen from solar energy, and to standardize the work in Rhizobials. |
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| + | Go back to our [[:Team:UNAM-Genomics_Mexico| main page]] or see our [[::Team:UNAM-Genomics_Mexico/Safety| safety questions]]. |
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| - | <li class="istylei0"><a class="istylei0" href="https://2011.igem.org/Team:UNAM-Genomics_Mexico">H2 Homepage</a></li>
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| - | <li class="istylei0"><a class="istylei0" href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Project"><span>Project</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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| - | <li><a href="#"><span>Intro</span><![if gt IE 6]></a>
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| - | <li><a href="https://2011.igem.org/User:Jurquiza">Uriel</a></li>
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| - | <li class="istylei0"><a class="istylei0" href="#"><span>Modeling</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]--></li>
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| - | <li class="istylei0"><a class="istylei0" href="#"><span>Human Practices</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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| - | <li><a href="#"><span>Ethics</span><![if gt IE 6]></a>
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| - | <li><a href="https://2011.igem.org/Team:UNAM-Genomics_Mexico/Bio-SintetizArte">Call</a></li>
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| - | <li><a href="#">Photos</a></li>
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| - | <li class="istylei0"><a class="istylei0" href="#">Sponsors</a></li>
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| - | <div class="left_image">
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| - | <img class="sub_project" alt="Our Project Image" src="https://static.igem.org/mediawiki/2011/d/dd/GPPU_in_the_lab_500x500.jpg" title="Wet lab">
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| - | </div>
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| - | <div class="summary_text">
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| - | <p><a class="project" href=""><i>Rhizobium etli</i></a> is a bacterial species that can live either freely or in symbiosis with the bean <a class="project" href=""><i>Phaseolus vulgaris</i></a>.
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| - | In it's symbiotic stage <i>R. etli</i> lives in nodules in the plant's root and has the ability to <a class="project" href="">fixate nitrogen</a> gas as amine molecules.
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| - | Inside the nodule the environment is <a class="project" href="">hypoxigenic</a>.
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| - | The <a class="project" href="">enzymatic production of hydrogen</a> requires a hypoxigenic environment to favor the reactions.
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| - | We want to introduce the metabolic pathway of hydrogen production into <i>R. etli</i> and take advantage of its niche in the hypoxic nodule.
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| - | Our goal is <a class="project" href="">hydrogen</a> production in the <i>Phaseolus vulgaris</i> root.</p>
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| - | </div>
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| - | <div class="descriptive_text">
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| - | <p>These are the goals in our project:</p>
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| - | <h1 class="project_goals">System design</h1>
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| - | <p>The system comprises four genes: a hydrogenase <a class="project" href=""><i>HydA</i></a>, a pyruvate ferrodoxin reductase
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| - | <a class="project" href=""><i>PFOR</i></a>, and two hydrogenase maturases <a class="project" href=""><i>HydEF</i></a> and
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| - | <a class="project" href=""><i>HydG</i></a>.</p>
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| - | <h1 class="project_goals">System insertion into <i>E. coli</i></h1>
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| - | <p><i>Rhizobium etli</i> can't be transformed, and it's phages haven't been studied enough.
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| - | However <i>R. etli</i> can receive DNA if conjugated with <i>Escherichia coli</i>.
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| - | To introduce our system into <i>R. etli</i> we must first introduce it into <i>E. coli</i>.</p>
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| - | <h1 class="project_goals">System conjugation into <i>R. etli</i></h1>
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| - | <p> The <a class="project" href="">conjugation process</a>.</p>
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| - | <h1 class="project_goals">Bean innoculation</h1>
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| - | <p> Our bacteria are innoculated in <a class="project" href="">bean seeds</a>.
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| - | These seeds are <a class="project" href="">grown</a> for several days.
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| - | When we have a small <a class="project" href="">nodulating plant</a> we will measure <a class="project" href="">hydrogen production</a>.</p>
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==Abstract==
Molecular hydrogen is an alternative energy carrier to electricity and hydrocarbon derived fuels. Among the natural systems that produce hydrogen, the most efficient ones are those that produce it through reactions catalyzed by enzymes with iron-sulfur clusters which require hypoxic microenvironments to work. The bacterium Rhizobium etli, during its symbiotic relationship with the common bean Phaseolus vulgaris, can transform nitrogen gas (N2) into ammonia (NH3) in a process called nitrogen fixation. The bean plant benefits from the produced nitrogen containing compounds. In exchange it provides the bacteria with carbon sources and a protected niche inside its root, where Rhizobium etli reaches a hypoxic state. We will take advantage of this hypoxic state to produce hydrogen in Rhizobium etli while maintaining nitrogen fixation.
Rhizobium etli is not commonly used in biological engineering. Thus we will both develop new tools and characterize parts already existing in the registry to work in a standardized way with Rhizobium etli. Our tools include plasmids, regulatory upstream regions and transcriptional terminators. The hydrogen-production pathway includes elements from the bacteria Clostridium acetobutylicum and Desulfovibrio africanus, and the algae Chlamydomonas reinhardtii. The two main goals of our project are to make Rhizobium etli a powerful agent in environmental protection by nitrifying soils and producing hydrogen from solar energy, and to standardize the work in Rhizobials.
Go back to our main page or see our safety questions.
"
