Team:KAIST-Korea/Projects/report 4-Test

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<p>&nbsp; We assume that the E. coli is in a darkroom for discovering the minimum number of fluorescent protein. In this reason, we use 0.1 lx, the minimum intensity of light that cone cells in human can perceive. <sup>2</sup> In fig1 (a), There is a brief picture for minimum visible acuity. We choose that 1 second is the basic time scale, so have to know the number of fluorescent protein per 1 second. By our research, The range of the photon emitted time is wide, from</p>
<p>&nbsp; We assume that the E. coli is in a darkroom for discovering the minimum number of fluorescent protein. In this reason, we use 0.1 lx, the minimum intensity of light that cone cells in human can perceive. <sup>2</sup> In fig1 (a), There is a brief picture for minimum visible acuity. We choose that 1 second is the basic time scale, so have to know the number of fluorescent protein per 1 second. By our research, The range of the photon emitted time is wide, from</p>
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1. Overview </br>
 
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Our system, E.Casso is composed of two modules. Each module is a different strain of E. coli. We introduced the modules into disparate strains of E.coli because it is usually easier to engineer something by adopting the ‘divide and conquer’ strategy. The modules perform the following tasks: The first type (Brush E. coli) produces signals that determine the color among green, cyan, yellow, and red generated by adjacent, second type of E. coli. This is achieved through an inherent mechanism by which E. coli naturally communicate with each other, the quorum sensing. By exchanging signaling molecules termed quorum, E. coli can coordinate gene expression as a colony according to its local density. The second type (Dyestuff E. coli) receives quorum from the first type and produces corresponding fluorescent proteins. It also amplifies the signal made by the type 1 module and propagates it to the surrounding E. coli. In essence, we utilize cell-cell communication to coordinate the collective behavior of E. coli.
 
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This is akin to soaking a brush with any one of four colors and compressing it firmly against a point on a paper. As time goes by, the blob of paint on the paper will spread. Our genetically engineered E. coli will draw abstract paintings in a similar manner.
 
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The modeling procedure is divided into four parts.
 
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A.Modeling the production of quorum by the first type of E. coli (Brush E. coli): Objective – observe that Brush E. coli produce enough quorums per some interval of time.
 
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B.Modeling the diffusion of quorum: Objective – note the time scale in which quorum propagates from Brush E. coli to Dyestuff E. coli.
 
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C.Modeling the production of fluorescent proteins by the Dyestuff E. coli upon receiving quorum and projecting the time it takes for a noticeable amount of fluorescence to accumulate: Objective – observe the time it takes for enough fluorescence to build up.
 
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D.Modeling how the variation in E. coli distribution affects the final outcome: Objective – note how the ratio of the two E. coli and the method of seeding affect the resultant painting.
 
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Revision as of 16:17, 11 July 2011





















  Introduction


  The human eyes cannot perceive objects that are smaller than a certain size. Also, they cannot recognize light whose intensity is lower than an inherent threshold. We take these limitations into account to determine the number of fluorescent proteins that must accumulate before we can notice any fluorescence, and establish the minimum circular area required for us to perceive any fluorescence.


  Objective


  Investigate concentration required for an amount of fluorescent protein in the E.coli that makes light from E.coli be visible for human.


  Background


  Human has the limits in vision. For our objective, we have to know about the limit of recognizing size of objects in human vision. This limit is called the ‘Minimum visible acuity’. The exact definition of minimum visible acuity is the minimum size of object that the human eyes can discern. In the table 1 Types of visual acuity(reference 1), the value of detection acuity(red box), ~1.0 arc second, is the minimum visible acuity that we take.

  Table : Types of visual acuity 1

  We assume that the E. coli is in a darkroom for discovering the minimum number of fluorescent protein. In this reason, we use 0.1 lx, the minimum intensity of light that cone cells in human can perceive. 2 In fig1 (a), There is a brief picture for minimum visible acuity. We choose that 1 second is the basic time scale, so have to know the number of fluorescent protein per 1 second. By our research, The range of the photon emitted time is wide, from

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