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Team:Freiburg/Notebook/15 June
From 2011.igem.org
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Transformation of BBa_K124014 bacteriophage Holin Gene pS105<br/> | Transformation of BBa_K124014 bacteriophage Holin Gene pS105<br/> | ||
plated out at 3:36pm in plates with ampicillin | plated out at 3:36pm in plates with ampicillin | ||
| + | <br/> | ||
| + | <br/> | ||
| + | ==3A-assemby:Digestion== | ||
| + | |||
| + | '''Investigators: Theo, Rüdiger''' | ||
| + | |||
| + | Restriction digest of plasmid constructs (3A Assemblies PR1, PR2, PR3, PR5, PR7) | ||
| + | Protocol: Mix => for one reaction, total volume 10µl | ||
| + | 3µl DNA | ||
| + | 1µl NEBuffer4 | ||
| + | 1µl BSA | ||
| + | 0,5µl Enzym | ||
Revision as of 09:19, 11 July 2011
Contents |
??.Labday
PCR for Ccas and CcaR
Investigators: Julia
Ccas: cyanobacteriochrome is a green light receptor that induces the expression of pycobillisome linker protein
Ccar: cognate response regulator
Primer diluted, PCR for CcaS with P(rimer) 5, P7 and CcaR with P4, P6
32,5 µl H2O
2µl Primer fw
2µl Primer rev
1µl DNA ( synechocystis from Hess-Lab)
1µl dNTPs
0,5 µl Phusion
Miniprep
Investigators: Julia, Sandra
Comments: Miniprep from june,14 was showed bad results. Grew over night at 37°C.
Miniprep PR4a, PR4b, PR8a, PR8b, PR9a, PR9b
measurement of nanodrop was ok
Transformation
Investigators: Sandra
Transformation of BBa_K124014 bacteriophage Holin Gene pS105
plated out at 3:36pm in plates with ampicillin
3A-assemby:Digestion
Investigators: Theo, Rüdiger
Restriction digest of plasmid constructs (3A Assemblies PR1, PR2, PR3, PR5, PR7) Protocol: Mix => for one reaction, total volume 10µl 3µl DNA 1µl NEBuffer4 1µl BSA 0,5µl Enzym
