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Team:Freiburg/Notebook/12 June
From 2011.igem.org
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Colonies were picked from plates PR1, PR2, PR3 PR5 and PR7. LB/agar (with 0,5microl Chlorampenicol/ml LB) was inoculated with these cells. | Colonies were picked from plates PR1, PR2, PR3 PR5 and PR7. LB/agar (with 0,5microl Chlorampenicol/ml LB) was inoculated with these cells. | ||
Incubation: Overnight at 37C 140RPM (Start: 18.30, End: next day 12:15) | Incubation: Overnight at 37C 140RPM (Start: 18.30, End: next day 12:15) | ||
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| + | ==Amplification of the red-light sensor== | ||
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| + | '''Investigators: Theo, Sophie''' | ||
| + | |||
| + | amplified the parts for the red light sensor I15008 and I15009 in LB with 1ul/ml Kanamycin , Overnight at 37C 140RPM<br/> | ||
| + | (Start: 18.30, End: 12:15) | ||
| + | for the main receptor ( BBa_I15010) we have no DNA. | ||
Revision as of 08:24, 11 July 2011
??.Labday
Picking clones
Investigators: Theo, Sophie
Comments: On plates PR4, PR6, PR8 and PR 9 cells did not grow. More competent cells were plated out again. Succesful growing on plates PR1, PR2, PR3, PR5 and PR7
Colonies were picked from plates PR1, PR2, PR3 PR5 and PR7. LB/agar (with 0,5microl Chlorampenicol/ml LB) was inoculated with these cells. Incubation: Overnight at 37C 140RPM (Start: 18.30, End: next day 12:15)
Amplification of the red-light sensor
Investigators: Theo, Sophie
amplified the parts for the red light sensor I15008 and I15009 in LB with 1ul/ml Kanamycin , Overnight at 37C 140RPM
(Start: 18.30, End: 12:15)
for the main receptor ( BBa_I15010) we have no DNA.
