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Team:Cambridge/Experiments
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===Preliminary Exercise=== | ===Preliminary Exercise=== | ||
| - | [[File:CamGFPplasmid. | + | [[File:CamGFPplasmid.TIFF |thumb|400px|right|The plasmid in which the bacillus gene would be fused with GFP.]] |
Our initial task was to find an interesting protein in Bacillus, and design the appropriate primers to make a GFP fusion to this protein. Then, we used the process of [[Team:Cambridge/Protocols/Gibson_Assembly | Gibson Assembly]] to create our fusion. | Our initial task was to find an interesting protein in Bacillus, and design the appropriate primers to make a GFP fusion to this protein. Then, we used the process of [[Team:Cambridge/Protocols/Gibson_Assembly | Gibson Assembly]] to create our fusion. | ||
Revision as of 09:41, 10 July 2011
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Experiments
Details of the experiments carried out throughout the project are linked from this page. These experiments should also be linked to from the appropriate diary page.
Preliminary Exercise
File:CamGFPplasmid.TIFF Our initial task was to find an interesting protein in Bacillus, and design the appropriate primers to make a GFP fusion to this protein. Then, we used the process of Gibson Assembly to create our fusion.
This exercise introduced many of us to laboratory work for the first time, and helped reinforce the key concepts and 'central dogma' as well as practice reading research papers.
The group was divided into three for this exercise; each group worked independently to create a separate gene fusion.
To do: add diagram of plasmid vector (appended to ApE file)
