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Team:EPF-Lausanne/Protocols/Primers preparation
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When receiving new primers, we need to add water in them on order to achieve 50 microM concentration. One rapid method is to: | When receiving new primers, we need to add water in them on order to achieve 50 microM concentration. One rapid method is to: | ||
* take the value "nmol yield" on the synthesis report and multiply it 20x to have the needed microliters of water | * take the value "nmol yield" on the synthesis report and multiply it 20x to have the needed microliters of water | ||
* spin down the primers tube to be sure that all DNA is at the bottom | * spin down the primers tube to be sure that all DNA is at the bottom | ||
* add the calculated amount of water in each tube, then mix them | * add the calculated amount of water in each tube, then mix them | ||
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| + | {{:Team:EPF-Lausanne/Templates/Footer}} | ||
Revision as of 15:09, 8 July 2011
Primers preparation
When receiving new primers, we need to add water in them on order to achieve 50 microM concentration. One rapid method is to:
- take the value "nmol yield" on the synthesis report and multiply it 20x to have the needed microliters of water
- spin down the primers tube to be sure that all DNA is at the bottom
- add the calculated amount of water in each tube, then mix them
