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Team:Nevada/Notebook/Temp
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<font color=red>E. Coli <br> | <font color=red>E. Coli <br> | ||
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Cyano <br> | Cyano <br> | ||
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| + | Enzymology<br> | ||
Hexokinase coupled assay used to quantitate amounts of cyanobacteria glucose secretion. | Hexokinase coupled assay used to quantitate amounts of cyanobacteria glucose secretion. | ||
Revision as of 19:48, 7 July 2011
Contents |
Temp Calender
Week 1 - June 1st-5th
E. Coli
Comment Here
Cyano ==Week 5 - June 27th - July 3rd==
Comment Here
<p>
Enzymology
Hexokinase coupled assay used to quantitate amounts of cyanobacteria glucose secretion.
* Rxn: 1. glucose + ATP --Hx-> G-6-P + ADP
* 2. G-6-P + NAD+ --G-6-PDeH-> G-6-P + NADH
Reaction used Glucose assay kit (Genzyme) containing 2000U/L Hexokinase reagent and 4000U/L G-6-P DeH and measured glucose concentrations ranging from 0.05mM-0.25mM and absorbances were measured at 340.0 nm.
Results: Final NADH concentrations were determined using the molar extinction coefficient and were proportionate to initial glucose concentrations. Discussion: Focus is now to measure not only glucose, but fructose concentrations as well as both with be secreted in the form of sucrose using a D-glucose/D-fructose assay (Megaenzyme.
Media
Week 2 - June 6th-12th
Week 3 - June 13th-19th
Week 4 - June 20th-26th
