Team:Paris Liliane Bettencourt/Notebook/2011/07/06/

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(Difference between revisions)
(Created page with "<p style="display:block;"><font color="red">Babak</font> </p><p style="display:block;"><u>gel electrophoresis at 0.6% of Agarose</u> </p> <ul><li>well 1: ladder 100pb </li><li>w...")
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</li><li>well 4: BBa_E0040 cut by XbaI and Pst1  
</li><li>well 4: BBa_E0040 cut by XbaI and Pst1  
</li><li>well 6: pHyperSpank,121pb, cut by XbaI and Pst1, didn't work
</li><li>well 6: pHyperSpank,121pb, cut by XbaI and Pst1, didn't work
 +
 +
<p style="display:block;"><u>gel purification</u>
 +
</p>
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<ul><li>well 1: ladder 100pb
 +
</li><li>from well 2: BBa_B4 pVeg+ SpoVg  cut by EcoR1 and SpeI, concentration of DNA=6,3ng/µL after refiltration
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</li><li>from well 4: BBa_E0040 cut by XbaI and Pst1,concentration of DNA=13ng/µL
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</li></ul>

Revision as of 15:15, 7 July 2011

Babak

gel electrophoresis at 0.6% of Agarose

  • well 1: ladder 100pb
  • well 2: BBa_B4 pVeg+ SpoVg cut by EcoR1 and SpeI
  • well 4: BBa_E0040 cut by XbaI and Pst1
  • well 6: pHyperSpank,121pb, cut by XbaI and Pst1, didn't work

    gel purification

    • well 1: ladder 100pb
    • from well 2: BBa_B4 pVeg+ SpoVg cut by EcoR1 and SpeI, concentration of DNA=6,3ng/µL after refiltration
    • from well 4: BBa_E0040 cut by XbaI and Pst1,concentration of DNA=13ng/µL