"
Team:Paris Liliane Bettencourt/Notebook/2011/07/05/
From 2011.igem.org
(Difference between revisions)
(→ligation and transformation) |
(→ligation and transformation) |
||
| Line 14: | Line 14: | ||
</li></ul> | </li></ul> | ||
| - | <p style="display:block;"><u>Digestion</u><ul><li>BBa_K090403 cut by EcoR1 and XbaI | + | <p style="display:block;"><u>Digestion</u> |
| - | </li><li> | + | <ul><li>BBa_K090403 cut by EcoR1 and XbaI |
| - | </li><li> | + | </li><li>BBa_SB1C3,2070pb, cut by EcoR1 and Ptsx |
| - | </li><li> | + | </li><li>BBa_J18913,2428pb, cut by EcoR1 and XbaI |
| + | </li><li>BBa_pHyperspank,121pb, cut by Xbal and Ptsx | ||
</li><li>B4 pVeg + SpoVg,117pb, cut by EcoR1 and SpeI | </li><li>B4 pVeg + SpoVg,117pb, cut by EcoR1 and SpeI | ||
</li></ul> | </li></ul> | ||
Revision as of 14:26, 7 July 2011
ligation and transformation
ligation
- BBa_k143079 6.4ng/µL in BBa_K090403 (vector) 9.2ng/µL
- BBa_K145001 8.5ng/µL - BBa_B0015 (in the vector) 10.9ng/µL
Babak
Gel purification
- well 1: ladder 100pb
- from well 2: BBa_B1010 cut by EcoR1 and S, concentration of DNA=8,9ng/µL
Digestion
- BBa_K090403 cut by EcoR1 and XbaI
- BBa_SB1C3,2070pb, cut by EcoR1 and Ptsx
- BBa_J18913,2428pb, cut by EcoR1 and XbaI
- BBa_pHyperspank,121pb, cut by Xbal and Ptsx
- B4 pVeg + SpoVg,117pb, cut by EcoR1 and SpeI
