Team:Lethbridge/Notebook

• JV & DG
  
• AV & IW
  
• JS & RP
  
• HB & BL
  
• MC & BV
  
• KG & SD
  

Assignments

April 9th - April 30th, 2011

Group 1 (JV, DG, AV, BA, IW)

1. Assembly Round 1
   1. K331033 + K331035
   2. R0040 + K331023 ==> K331031 (Already Made)
   3. K331025 + B0015
   4. R0040 + K331039
   5. K331039 + B0015
2. Assembly Round 2
   1. 1.2 + 1.3
   2. 1.4 + B0015
   3. R0040 + 1.4

Characterize Fluorescence of Parts 1.1, 2.1, 2.2, 2.3

Group 2 (HB, BL, MC, BV)

1. Assembly Round 1
   1. R0010 + B0034
   2. K249002 + B0015
   3. I13452 + B0034
   4. C0040 + B0015
2. Assembly Round 2
   1. 1.1 + 1.2
   2. 1.3 + 1.4
3. Assembly Round 3
   1. 2.1 + 2.2

Group 3 (JS, RP, KG, CC, SD)

• Use PCR to alter suffixes to include fusion standards for xylE and mms6
• Assemble fusion-ready xylE and mms6 with S04261 (C-terminal Arginine tail + B0015)
• Move these BioBricks from synthesis vectors (either pMA-T or pMA-RQ) into pSB1C3
  • K331007
  • K331008
  • K331022
  • K331024
  • K331025

Protocols