Team:Macquarie Australia

From 2011.igem.org

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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
 
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
 
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:Macquarie_Australia_logo.png|200px|right|frame]]
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|[[File:Macquarie_Australia_logo.png|200px|right|frame]]
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''Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)''
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'''Introducing a functional light switch into E. coli. '''
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The objective in this project is to build and characterise a biological light switch in E.coli. This will involve construction of bacteriophytochrome biobrick parts and heme-oxygenase biobrick parts. In 2010 the Macquarie Team cloned bacteriophytochrome from two sources and showed that one was functionally assembled when incubated with biliverdin. The part created is not directly usable as a biobrick as it contains an internal PstI site and the XbaI biobrick site is missing. The heme-oxygenase clone also contains an internal restriction site which is not compatible with biobrick assembly.
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|[[Image:Macquarie_Australia_team.png|right|frame|Your team picture]]
|[[Image:Macquarie_Australia_team.png|right|frame|Your team picture]]
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|align="center"|[[Team:Macquarie_Australia | Team Example]]
 
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<!--- The Mission, Experiments --->
<!--- The Mission, Experiments --->
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The aims of the team this year are to complete the light switch construction:
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<br>
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1. Remove the restriction sites from the bacteriophytochrome and heme-oxygenase genes which are incompatible with biobrick assembly.
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<br>
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2. Assemble a fully functional bacteriophytochrome biobrick which is functionally expressed in E.coli.
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3. Assemble an operon consisting of the heme-oxygenase and bacteriophytochrome genes.
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4. Optimise the gene expression from the operon such that the bacteriophytochrome light switch assembles without requiring the addition of biliverdin.
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<br>
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Revision as of 05:09, 7 July 2011

You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
Macquarie Australia logo.png

Introducing a functional light switch into E. coli. The objective in this project is to build and characterise a biological light switch in E.coli. This will involve construction of bacteriophytochrome biobrick parts and heme-oxygenase biobrick parts. In 2010 the Macquarie Team cloned bacteriophytochrome from two sources and showed that one was functionally assembled when incubated with biliverdin. The part created is not directly usable as a biobrick as it contains an internal PstI site and the XbaI biobrick site is missing. The heme-oxygenase clone also contains an internal restriction site which is not compatible with biobrick assembly.

File:Macquarie Australia team.png
Your team picture

The aims of the team this year are to complete the light switch construction:
1. Remove the restriction sites from the bacteriophytochrome and heme-oxygenase genes which are incompatible with biobrick assembly.
2. Assemble a fully functional bacteriophytochrome biobrick which is functionally expressed in E.coli.
3. Assemble an operon consisting of the heme-oxygenase and bacteriophytochrome genes.
4. Optimise the gene expression from the operon such that the bacteriophytochrome light switch assembles without requiring the addition of biliverdin.


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