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Team:Amsterdam/6 July 2011
From 2011.igem.org
(Difference between revisions)
(→Ideas for modeling) |
(→Steady state model of promoters) |
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***Transcription | ***Transcription | ||
****Measuring with qPCR | ****Measuring with qPCR | ||
| - | **Translation | + | ***Translation |
| - | ***Fluorescence | + | ****Fluorescence |
**Growth rate | **Growth rate | ||
*Information needed for all promoters | *Information needed for all promoters | ||
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** The inducible promoters are also concentration dependent | ** The inducible promoters are also concentration dependent | ||
*** Need to look into literature for already available information | *** Need to look into literature for already available information | ||
| + | |||
==The cold chock promoter== | ==The cold chock promoter== | ||
*How does this promoter work? | *How does this promoter work? | ||
Revision as of 12:08, 6 July 2011
Contents |
Ideas for modelling
Brainstorm session with Douwe Molenaar and Isoude Kuijper
Steady state model of promoters
- Information needed for model
- Stability
- Transcription
- Measuring with qPCR
- Translation
- Fluorescence
- Transcription
- Growth rate
- Stability
- Information needed for all promoters
- Inducible promoter
- IPTG promoter is also dependable on a lactose receptor/transporter
- Cold shock promoter
- Inducible promoter
- Temperature dependent
- The inducible promoters are also concentration dependent
- Need to look into literature for already available information
- The inducible promoters are also concentration dependent
The cold chock promoter
- How does this promoter work?
Cryobrick
- What concentration is needed?
- How many targets do the chaperone proteins have?
Enzyme activity
- Enzyme activity is also temperature dependent
