Team:Lethbridge/Notebook

From 2011.igem.org

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==<font color="white">Group Member Pages==
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*<font size=3><b>[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group1 JV & DG]<br>
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*[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group2 AV & IW]<br>
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*[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group3 JS & RP]<br>
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*[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group4 HB & BL]<br>
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*[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group5 MC & BV]<br>
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*[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group6 KG & SD]<br></b></font>
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==<font color="white">Assignments==
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==Assignments==
===April 9th - April 30th, 2011===
===April 9th - April 30th, 2011===
====Group 1 (JV, DG, AV, BA, IW)====
====Group 1 (JV, DG, AV, BA, IW)====
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**K331025
**K331025
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==Group Member Pages==
 
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===[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group1 JV & DG]===
 
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===[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group2 AV & IW]===
 
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===[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group3 JS & RP]===
 
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===[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group4 HB & BL]===
 
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===[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group5 MC & BV]===
 
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===[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group6 KG & SD]===
 
==Protocols==
==Protocols==
<br><br>
<br><br>

Revision as of 01:01, 10 April 2011



Contents

Group Member Pages

Assignments

April 9th - April 30th, 2011

Group 1 (JV, DG, AV, BA, IW)

  1. Assembly Round 1
    1. K331033 + K331035
    2. R0040 + K331023 ==> K331031 (Already Made)
    3. K331025 + B0015
    4. R0040 + K331039
    5. K331039 + B0015
  2. Assembly Round 2
    1. 1.2 + 1.3
    2. 1.4 + B0015
    3. R0040 + 1.4

Characterize Fluorescence of Parts 1.1, 2.1, 2.2, 2.3

Group 2 (HB, BL, MC, BV)

  1. Assembly Round 1
    1. R0010 + B0034
    2. K249002 + B0015
    3. I13452 + B0034
    4. C0040 + B0015
  2. Assembly Round 2
    1. 1.1 + 1.2
    2. 1.3 + 1.4
  3. Assembly Round 3
    1. 2.1 + 2.2

Group 3 (JS, RP, KG, CC, SD)

  • Use PCR to alter suffixes to include fusion standards for xylE and mms6
  • Assemble fusion-ready xylE and mms6 with S04261 (C-terminal Arginine tail + B0015)
  • Move these BioBricks from synthesis vectors (either pMA-T or pMA-RQ) into pSB1C3
    • K331007
    • K331008
    • K331009
    • K331012
    • K331022
    • K331024
    • K331025

Protocols

Retrieved from "http://2011.igem.org/Team:Lethbridge/Notebook"