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Team:Paris Liliane Bettencourt/Notebook/2011/07/04/
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Revision as of 09:45, 5 July 2011
Minipreps, digestion, purification and ligation
Axel
Minipreps
- on BBa_K090403 (vector), concentration of DNA=248.8ng/µL
- on BBa_K143079 (device with constitutive promoter and GFP), concentration of DNA=500.2ng/µL
- on BBa_B0015, concentration of DNA=408.1ng/µL
- on BBa_E1010 concentration of DNA=330.8ng/µL
- on BBa_K145001, concentration of DNA=572.8ng/µL
10 µL of minipreped DNA corresponds to approximately 125ng of DNA (see fluorescence of the 3Kb band of the ladder)
digestion
- BBa_K090403 cut by EcoR1 and XbaI
- BBa_K143079 cut by EcoR1 and SpeI
- BBa_B0015 cut by EcoR1 and XbaI
- BBa_E1010 cut by EcoR1 and SpeI
- BBa_K145001 cut by EcoR1 and SpeI
gel electrophoresis at 0.6% of Agarose
- well 1: ladder 1kb
- well 2: BBa_B0015 cut by EcoR1 and XbaI
- well 3: BBa_E1010 cut by EcoR1 and SpeI
- well 4: BBa_K145001 cut by EcoR1 and SpeI
- well 5: BBa_K090403 cut by EcoR1 and XbaI
- well 6: BBa_K143033 cut by EcoR1 and SpeI
<a href="/wiki/Image:electrophoresis 040711.jpg" class="image" title="Image:electrophoresis 040711.jpg"><img alt="Image:electrophoresis 040711.jpg" src="/images/9/99/electrophoresis 040711.jpg" width="425" height="539" border="0" /></a>
