Team:Paris Liliane Bettencourt/Notebook/2011/07/04/

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Minipreps, digestion, purification and ligation

Axel

Minipreps

  • on BBa_K090403 (vector), concentration of DNA=248.8ng/µL
  • on BBa_K143079 (device with constitutive promoter and GFP), concentration of DNA=500.2ng/µL
  • on BBa_B0015, concentration of DNA=408.1ng/µL
  • on BBa_E1010 concentration of DNA=330.8ng/µL
  • on BBa_K145001, concentration of DNA=572.8ng/µL



10 µL of minipreped DNA corresponds to approximately 125ng of DNA (see fluorescence of the 3Kb band of the ladder)

</p>

digestion

  • BBa_K090403 cut by EcoR1 and XbaI
    • BBa_K143079 cut by EcoR1 and SpeI
      • BBa_B0015 cut by EcoR1 and XbaI
        • BBa_E1010 cut by EcoR1 and SpeI
          • BBa_K145001 cut by EcoR1 and SpeI
          </p>

          gel electrophoresis at 0.6% of Agarose

          • BBa_K090403 cut by EcoR1 and XbaI
            • BBa_K143079 cut by EcoR1 and SpeI
              • BBa_B0015 cut by EcoR1 and XbaI
                • BBa_E1010 cut by EcoR1 and SpeI
                  • BBa_K145001 cut by EcoR1 and SpeI
                  Electrophoresis 040711.jpg
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