Team:NTNU Trondheim/Protocols

From 2011.igem.org

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Line 9: Line 9:
*NaCl   5 g/L
*NaCl   5 g/L
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Autoclave before use
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Adjust pH to 7,4 with NaOH, autoclave.
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==LA==
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*Dissolve LB
 +
*Add agar, 15 g/L
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 +
Adjust pH to 7,4 with NaOH, autoclave.
 +
 
 +
 

Revision as of 07:48, 5 July 2011


Protocols / Recipes used in the lab

LB Broth

  • Bacto-tryptone: 10 g/L
  • Yeast extract: 5 g/L
  • NaCl 5 g/L

Adjust pH to 7,4 with NaOH, autoclave.

LA

  • Dissolve LB
  • Add agar, 15 g/L

Adjust pH to 7,4 with NaOH, autoclave.