From 2011.igem.org

Revision as of 03:18, 29 October 2011

ITESM MÉXICO

SensE.coli

Results

After returning from Indianapolis, the team continued to work as intended, however most of the materials had been already exhausted and the viability of the competent cells was questionable after more than 50 recultures. We suspected that after so many recultures the bacteria were no longer competent for transformation; to check this we prepared minipreps in order to review if the pieces were on the cells.

PCR product analysis showing the last status of the parts within the cells. Next image is a description of the gel.

Picture of the last cell culture made on 28/Oct/11 Pink colonies are untransformed bacteria/White colonies are transformed bacteria. Although the number of sucessfully transformed colonies is small, they were picked up and grown in another media.

Due to the complications post successfull ligation of the parts, we will use two procedures to ensure that the parts are present in the cells: PCR and DNA sequencing.

ID Name Sequence # bp

BS1F TGCCACCTGACGTCTAAGAA 20

BS1R CGGAAGATTCTGGTCCGTAG 20

BS2F GTTCCATGGATGTGGAAACC 20

BS2R CAGCGATTTTGTTCTTCACC 20

BS3F GGGTAACCTGAAGCAGTCCA 20

BS3R AACCGTATTACCGCCTTTGA 20

no enviadas

crxst y rbs

si enviadas

RecA Tast crwk