Team:Grenoble/Notebook/October

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<h2>Biology</h2>
<h2>Biology</h2>
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The aim of our experiments is to demonstration of the coloration, for this we have done different experiments:
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<p>
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The aim of our experiments is to demonstration of the coloration, for this we have done different experiments:
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</p>
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                                <p>
The goal is to introduce the constructions (pcst-RBS-CinR) and (pCin-lycopene) on the same bacterium, the constructions are supported on pSB3C5 and pSB1A2 respectively., by adding externel AHL molecule, this latter can enter in the bacterium and forms with CinR molecules complex that allows the activation of Lycopene production, after few times we can see the red color.  
The goal is to introduce the constructions (pcst-RBS-CinR) and (pCin-lycopene) on the same bacterium, the constructions are supported on pSB3C5 and pSB1A2 respectively., by adding externel AHL molecule, this latter can enter in the bacterium and forms with CinR molecules complex that allows the activation of Lycopene production, after few times we can see the red color.  
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                                </p>
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CLONING OF THE CONSTRUCTION pCst-RBS-CinR IN pSB3C5:
CLONING OF THE CONSTRUCTION pCst-RBS-CinR IN pSB3C5:
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                                <ul>
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<li>Restriction using  EcoRI and Pst1.</li>
<li>Restriction using  EcoRI and Pst1.</li>
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<li>Ligation: standard protocol was performed; Pcst-RBS-CinR was inserted into pSB3C5 plasmids</li>
<li>Ligation: standard protocol was performed; Pcst-RBS-CinR was inserted into pSB3C5 plasmids</li>
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<li>Spreading over Petri dish.</li>
<li>Spreading over Petri dish.</li>
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<li>Better cloning rate (New stock of biobrick Miniprep)</li>
<li>Better cloning rate (New stock of biobrick Miniprep)</li>
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<li>Do checking, PCR cheking.</li>
<li>Do checking, PCR cheking.</li>
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<li>To confirm the result gel checking was performed.</li>
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<li>To confirm the result gel checking was performed.</li></ul>
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pCin-Lycopene CONSTRUCTION IN pSB1A2 PREPARATION
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                                        </p>

Revision as of 02:59, 29 October 2011

Grenoble 2011, Mercuro-Coli iGEM


October 2nd to 9th

October 10th to 17th

Biology

The aim of our experiments is to demonstration of the coloration, for this we have done different experiments:

The goal is to introduce the constructions (pcst-RBS-CinR) and (pCin-lycopene) on the same bacterium, the constructions are supported on pSB3C5 and pSB1A2 respectively., by adding externel AHL molecule, this latter can enter in the bacterium and forms with CinR molecules complex that allows the activation of Lycopene production, after few times we can see the red color.

CLONING OF THE CONSTRUCTION pCst-RBS-CinR IN pSB3C5:

  • Restriction using EcoRI and Pst1.
  • Ligation: standard protocol was performed; Pcst-RBS-CinR was inserted into pSB3C5 plasmids
  • Spreading over Petri dish.
  • Better cloning rate (New stock of biobrick Miniprep)
  • Do checking, PCR cheking.
  • To confirm the result gel checking was performed.

pCin-Lycopene CONSTRUCTION IN pSB1A2 PREPARATION

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