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Team:Caltech/Week 4
From 2011.igem.org
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==July 3== | ==July 3== | ||
| + | <p>Checked Gibson transformations<br/> | ||
| + | Plated remaining transformed cells<br/> | ||
| + | Made new dilutions of original enrichment cultures (BPA, nonylphenol, 17-alpha-ethinyl-estradiol, DDT)</p> | ||
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===Results=== | ===Results=== | ||
There was no growth on the transformed Gibson plates. Since we did a small reaction, we used all of the reaction in transforming competent cells. The Gibson assembly likely failed, as one of the negative control plates had a single colony. However, we have had trouble with our competent cells in the past and will try plating what we have left.<br/><br/> | There was no growth on the transformed Gibson plates. Since we did a small reaction, we used all of the reaction in transforming competent cells. The Gibson assembly likely failed, as one of the negative control plates had a single colony. However, we have had trouble with our competent cells in the past and will try plating what we have left.<br/><br/> | ||
Revision as of 04:45, 4 July 2011
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Project |
July 3Checked Gibson transformations ResultsThere was no growth on the transformed Gibson plates. Since we did a small reaction, we used all of the reaction in transforming competent cells. The Gibson assembly likely failed, as one of the negative control plates had a single colony. However, we have had trouble with our competent cells in the past and will try plating what we have left.
This WeekStart enrichment cultures from a previous iteration, as the latest dilutions remain clear.
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