Team:WHU-China/Project/Modeling
From 2011.igem.org
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<div style="font-size:24px;font-style:Bold">The Gene Circuit </div> | <div style="font-size:24px;font-style:Bold">The Gene Circuit </div> | ||
- | <img src="/wiki/images/c/cd/Oscillator5.png" width="80%" style="margin-left: | + | <img src="/wiki/images/c/cd/Oscillator5.png" width="80%" style="margin-left:10%"/> |
<p> | <p> | ||
- | + | This is our gene circuit designed to equip our E.coli with the ability to change color with time.</br> | |
- | + | The key players are LsrR and LsrK. The alternation of their expression results in the oscillating concentration of the reporter—GFP, and thus yield the periodically changing color. </br> | |
- | + | Theoretically, at the very beginning, LsrR is expressed and inhibits LsrK. After some time, LsrR inhibits itself and its concentration drops. Therefore LsrK has a chance to express and climbs due to its self enlarging. However, LsrK induces the reexpression of LsrR at the same time. In this way, their concentrations wave alternatively. C1’s function is to delay the oscillation and stabilize the system. | |
</p> | </p> | ||
</div> | </div> | ||
- | + | <div class="wide" id="wide2"> | |
+ | <div style="font-size:24px;font-style:Bold">Modeling</div> | ||
+ | <p> | ||
+ | In the part of waving time , there is a two nodes system , using the method of the mathematic theory of the ODE function and the random analysis. We get our model.</br> | ||
+ | First we generate a ODE to describe the system : | ||
+ | </p> | ||
+ | <img src="/wiki/images/c/c3/Model-1.png" style="margin-left:10%"/> | ||
+ | <p> | ||
+ | the solution diagram of the function is: | ||
+ | </p> | ||
+ | </div> | ||
Revision as of 02:01, 29 October 2011
![](/wiki/images/c/cd/Oscillator5.png)
This is our gene circuit designed to equip our E.coli with the ability to change color with time. The key players are LsrR and LsrK. The alternation of their expression results in the oscillating concentration of the reporter—GFP, and thus yield the periodically changing color. Theoretically, at the very beginning, LsrR is expressed and inhibits LsrK. After some time, LsrR inhibits itself and its concentration drops. Therefore LsrK has a chance to express and climbs due to its self enlarging. However, LsrK induces the reexpression of LsrR at the same time. In this way, their concentrations wave alternatively. C1’s function is to delay the oscillation and stabilize the system.
In the part of waving time , there is a two nodes system , using the method of the mathematic theory of the ODE function and the random analysis. We get our model. First we generate a ODE to describe the system :
![](/wiki/images/c/c3/Model-1.png)
the solution diagram of the function is: