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Team:IIT Madras/Project
From 2011.igem.org
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<font color="#8B0000"><b><u>Project Artemis</u></b></font><br/> | <font color="#8B0000"><b><u>Project Artemis</u></b></font><br/> | ||
<b>Artemis</b> is the Greek Goddess of Light, and is known as the “<b>Protector of the Vulnerable</b>”. Our team has designed an Expression vector based on the <b>Carbon Stress Buster</b> device, for improving yield of Recombinant protein in substrate limiting conditions.<br/><br/> | <b>Artemis</b> is the Greek Goddess of Light, and is known as the “<b>Protector of the Vulnerable</b>”. Our team has designed an Expression vector based on the <b>Carbon Stress Buster</b> device, for improving yield of Recombinant protein in substrate limiting conditions.<br/><br/> | ||
Revision as of 01:48, 29 October 2011
Project Artemis
Artemis is the Greek Goddess of Light, and is known as the “Protector of the Vulnerable”. Our team has designed an Expression vector based on the Carbon Stress Buster device, for improving yield of Recombinant protein in substrate limiting conditions.
Project Sunscreen
The energy advantage provided to cells due to GPR expression increases the growth rate of PR expressing cells. This difference in growth rate has been hypothesized to be used for screening transformed cells. We propose a plasmid vector for screening, where the antibiotic resistance gene is replaced by Proteorhodopsin generator. The following is the modified protocol for screening:
Artemis is the Greek Goddess of Light, and is known as the “Protector of the Vulnerable”. Our team has designed an Expression vector based on the Carbon Stress Buster device, for improving yield of Recombinant protein in substrate limiting conditions.
Project Sunscreen
The energy advantage provided to cells due to GPR expression increases the growth rate of PR expressing cells. This difference in growth rate has been hypothesized to be used for screening transformed cells. We propose a plasmid vector for screening, where the antibiotic resistance gene is replaced by Proteorhodopsin generator. The following is the modified protocol for screening:
- Clone the part of interest into the pSB1Pc cloning vector’s MCS.
- Transform bacterial cells (using standard protocol).
- Plate cells on Minimal media agar containing Retinal (10uM).
- Place the plate under green light (525 nm) and incubate at 37 C.
- Pick transformed colonies after 12 hours of incubation.
