Team:Queens Canada/Parts/Registry

BBa_K631039 - nahD: The coding sequence for the nahD protein, from pseudomonas peutida, capable of degrading napthalene. Expressing this in our worm may give it the ability to degrade naphthalene.

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Regulatory Constructs for C. elegans

BBa_K631035 - Str2: A promoter for AWC neurons, normally used for a 7-transmembrane olfactory receptor. We can use this as a promoter to integrate non-native GPCRs into the AWC neuron membrane.

BBa_K631001 - ODR-7: A promoter for a 7-transmembrane olfactory receptor in AWA olfactory neurons. This can be used for integrating non-native GPCRs. Length: 1503

BBa_K631002 - let-858: A promoter for a protein essential for the development of the worm. We used this in one of our composite parts to express fluorescence in the worm.

BBa_K631003 - GPC-1: A promoter for one of the gamma sub-units of the GPCR binding proteins. We used this for our fluorescence as well. Length: 2000

BBa_K631004 - GCY7: A promoter for a membrane guanylyl cyclase protein. This can be used for a non-native receptor or fluorescence reporter.

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Composite Constructs

BBa_K631031 - ODR7:OX1R:UNC-54: A composite part designed to place the non-native OX1R into the neurons of the worm using the ODR-7 promoter for the ability to detect cholorobenzoic acid.

BBa_K631032 - ODR7:ADBR2:UNC-54: A composite part designed to place the non-native ADBR2 into the neurons of the worm using the ODR-7 promoter for the ability to detect phenol (albuteroll). Length: 3666

BBa_K631033 - GCY7:ADBR2:UNC-54: Another composite part with ADBR2, under the GCY7 promoter.

BBa_K631037 - GPC-1:eCFP:UNC-54: This is a composite part for our reporter system using cyano-fluorescent protein under the GPC-1 promoter. Length: 3787

BBa_K631034 - let858:RFP:UNC-54:This is a composite part for our reporter system using red fluorescent protein under the let858 promoter.

BBa_K631038 - GCY-7:eCFP:UNC-54: This is a composite part for our reporter system using cyano-fluorescent protein under the GCY-7 promoter.

BBa_K631036 - eCFP:UNC-54: Eukaryotic cyano-fluorescent protein for the reporter system in our worm. Length: 1629.

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Complete List of parts

<groupparts>iGEM011 Queens_Canada</groupparts>

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-<span class="classpurple5"><a href="http://partsregistry.org/Part:BBa_K631005"><b>BBa_K631005- NPY1R_Muss     </b> </a></span><p>
+<span class="classpurple5"><a href="http://partsregistry.org/Part:BBa_K631005">BBa_K631005- NPY1R_Mus  </a></span><p>
 <regulartext>The coding sequence for the NPY1R protein. This is a GPCR from <i>Mus musculus</i>.</regulartext>
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+<span class="classpurple5"><a href="http://partsregistry.org/Part:BBa_K631006">BBa_K631006 - NPY1R_Rat </a></span><p>
 <regulartext><b> BBa_K631006 - NPY1R_Rat:</b>The coding sequence for the NPY1R protein from <i>Rattus norvegius</i>. This is also a GPCR .</regulartext><br>
 </div>