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Team:Grenoble/Projet/Device
From 2011.igem.org
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| - | <a href="#mercury" title="Click here"><img src="https://static.igem.org/mediawiki/2011/ | + | <a href="#mercury" title="Click here"><img src="https://static.igem.org/mediawiki/2011/0/02/Bouton_device_death.png"/></a> |
<div class="legend"> | <div class="legend"> | ||
Mercury poisoning. | Mercury poisoning. | ||
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<td> | <td> | ||
<center> | <center> | ||
| - | <a href="#device" title="Click here"><img src="https://static.igem.org/mediawiki/2011/ | + | <a href="#device" title="Click here"><img src="https://static.igem.org/mediawiki/2011/e/ea/Bouton_device_device.png"/></a> |
<div class="legend"> | <div class="legend"> | ||
| - | + | Design of the device. | |
</div> | </div> | ||
</center> | </center> | ||
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<td> | <td> | ||
<center> | <center> | ||
| - | <a href="#meeting" title="Click here"><img src="https://static.igem.org/mediawiki/2011/ | + | <a href="#meeting" title="Click here"><img src="https://static.igem.org/mediawiki/2011/c/cc/Bouton_HP_arkema.png"/></a> |
<div class="legend"> | <div class="legend"> | ||
Click on the icon above to know why RsmA is important. | Click on the icon above to know why RsmA is important. | ||
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<h2>Mercury poisoning.</h2> | <h2>Mercury poisoning.</h2> | ||
| + | <h3>Links</h3> | ||
| + | <a href="www.epa.gov/hg/effects.htm">www.epa.gov/hg/effects.htm</a> | ||
| - | </div | + | </div> |
| - | <div class="blocbackground"> | + | <div id="device" class="blocbackground"> |
<h2>Design and use of Mercuro-Coli device</h2> | <h2>Design and use of Mercuro-Coli device</h2> | ||
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</p> | </p> | ||
<p> | <p> | ||
| - | + | Each channel form a specific chemical environnement: they contain distincts concentration of IPTG, such that a gradient is obtained on the whole plate. | |
</p> | </p> | ||
<center><img height="280px" src="https://static.igem.org/mediawiki/2011/a/a2/IMG_5964_%28copie%29.JPG" class="bordure"/></center> | <center><img height="280px" src="https://static.igem.org/mediawiki/2011/a/a2/IMG_5964_%28copie%29.JPG" class="bordure"/></center> | ||
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Photography of a test prototype made to determine the depth required for a visible coloration. | Photography of a test prototype made to determine the depth required for a visible coloration. | ||
</div> | </div> | ||
| + | <p> | ||
| + | A graduation is etched below the channels to read the concentration corresponding to the channel where the red stripe appear. The graduation is not shown on the picture above. Experiments are made at the moment to know how to draw the graduation. Even if we have not all the data needed to get an experimental graduation, <a href="https://2011.igem.org/Team:Grenoble/Projet/Results/Device#graduation" title="Click here to learn more about it">our modelling work gave us that answer.</a> | ||
| + | </p> | ||
<h3>Use: prepare the sample, read the result</h3> | <h3>Use: prepare the sample, read the result</h3> | ||
Revision as of 21:39, 28 October 2011
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Real application of our device
In this section of the wiki, we present how we consider the design of our device and discuss realistic application. To learn more about the market expectations, we met a safety official of ARKEMA. Here are also some information about mecury toxicity.
Mercury poisoning.
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Design of the device.
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Click on the icon above to know why RsmA is important.
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Design and use of Mercuro-Coli device
Design
Our objective is to design a device for the detection and quantification of heavy metal pollution in a given sample. Our final device is intended to sense mercury, but for practical reasons, we mainly work with a harmless molecule as a proof of concept: anhydrotetracyclin. One should notice that most of the elements in the design of our device and genetic network would not change whatever the chemical that we could want to quantify.
The device presented here take into account the information provided by modelling results. The device we conceived is rectangular shape, thin, light and very cheap. It is made of machined polycarbonate. Vertical channels are engraved in the plastic.
Each channel form a specific chemical environnement: they contain distincts concentration of IPTG, such that a gradient is obtained on the whole plate.
A graduation is etched below the channels to read the concentration corresponding to the channel where the red stripe appear. The graduation is not shown on the picture above. Experiments are made at the moment to know how to draw the graduation. Even if we have not all the data needed to get an experimental graduation, our modelling work gave us that answer.
Use: prepare the sample, read the result
Packaging
Collect, then prepare the sample
Read the result
Disposal of the device
A sample preparation phase is needed. In fact mercury is generally not in ionic form in nature. It's generally complexed with others ions or for much of the amount of mercury it forms organometalics compound. It is because some bacteria metabolize Hg 2+ and transform it in methyl-mercury. This organic compound is eaten by fish. And fish is eaten by men. That's why mercury is so poisonous.
To use the device we just have to spread poluted water on it and wait for the result. Below is an illustration of how we observe results using the measurement device. One channel become red, the location give us the amount of mercury in the water.
The device is disposable. Once the test is performed, autoclave the device to sterilize it.
Meeting at ARKEMA and lab's visits
 iGEM 2011 Main Page Contact Us:Clic here ! |
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