Team:WHU-China/Project

    Being born, growing, aging and embracing death, the life process that every creature must go through is just like a movie. However, as one of the simplest life forms, E.coli's life story is too evanescent and monotonous. How can we utilize E.coli to show more complicated processes, such as the growing and withering of a tree?
     Numerous problems stand in the way to this goal. So we just take two steps towards this direction. Firstly, we only focus on one pixel of this picture. As time goes by, the color of this point changes periodically. This effect is achieved by a strain of E.coli working as an oscillator which can yield different kinds of pigment periodically with the help of a signal transformation system....

    This summer we designed two projects: color film and oscillator. One is about time and another is about space. The oscillator is to construct one strain colorful E.coli changing color periodically. The color film project is to construct three strains of E.coli which can form a colorful image. Here is the detailed design of these two projects.

As the changes of cycle time with the change of the parameters in the image , we can get the conclusion that:
     1.have little influence on the range.
     2.do not have much influence on the cycle time.
     3.have inversely proportional relationship with the cycle time , it have a huge influence on the cycle time , but little impact on the range (Can be used to adjust the cycle to maintain constant amplitude)
     4.When, have little influence on the range.
This conclusion we get before can be used to guide the design of biological systems, in order to achieve the adjustment period, amplitude can adjust the size of some parameters.........

   Blue light induced bacteria with part BBa_K560000, blue light sensing and reporting system. E.coli cultures from the same clone were grown in the dark under the blue light. Control were under the dark. Bacteria under blue light expressed little RFP and the bacterial colony did not turn red. Control bacterial colony turned red obviously.....