Team:Caltech/Week 4
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==This Week== | ==This Week== | ||
+ | <p>Try PCRing from source plate [http://partsregistry.org/Part:pSB4A5 pSB4A5] for Gibson<br/> | ||
+ | Gibson assemble pNT001 and pNT002 and negative controls following Joe's protocol.<br/> | ||
+ | Transform pNT001 and pNT002 into competent cells<br/> | ||
+ | Design experiments to test for degradation of BPA by pNT001 and pNT002 using the HPLC<br/> | ||
Get the BioBricks we ordered from the parts registry ([http://partsregistry.org/Part:BBA_K364309 K364309], [http://partsregistry.org/Part:BBA_K364329 K364329], [http://partsregistry.org/Part:BBA_K364327 K364327], [http://partsregistry.org/Part:BBA_K364307 K364307])<br/> | Get the BioBricks we ordered from the parts registry ([http://partsregistry.org/Part:BBA_K364309 K364309], [http://partsregistry.org/Part:BBA_K364329 K364329], [http://partsregistry.org/Part:BBA_K364327 K364327], [http://partsregistry.org/Part:BBA_K364307 K364307])<br/> | ||
Transform these into cells, miniprep and sequence<br/> | Transform these into cells, miniprep and sequence<br/> | ||
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Put LA River soil DNA into fosmids<br/> | Put LA River soil DNA into fosmids<br/> | ||
Work on Project Description and Safety Proposal<br/> | Work on Project Description and Safety Proposal<br/> | ||
- | Once we've sequenced ER ([http://partsregitry.org/Part:BBa_K123003 K123003]), start making test plasmids to see if it works. | + | Once we've sequenced ER ([http://partsregitry.org/Part:BBa_K123003 K123003]), start making test plasmids to see if it works.</p> |
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Revision as of 22:36, 1 July 2011
Project |
This WeekTry PCRing from source plate [http://partsregistry.org/Part:pSB4A5 pSB4A5] for Gibson
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