"
Team:British Columbia/Accomplishments
From 2011.igem.org
(Difference between revisions)
| Line 5: | Line 5: | ||
<div id="bod"></html> | <div id="bod"></html> | ||
| - | <font size=" | + | <font size="3"><h3>Wet Laboratory Achievements</h3></font> |
<hr style="color:#BDCBBD; height:3px;" /> | <hr style="color:#BDCBBD; height:3px;" /> | ||
<b></b> | <b></b> | ||
| - | <font size=" | + | <font size="2"><h3>1. In vitro assay production of monoterpene in bacteria</h3></font> |
| - | <b></b><h3>Geranyl Pyrophosphate (GPP) Assay</h3> | + | <b></b><font size="1"><h3>Geranyl Pyrophosphate (GPP) Assay</h3></font> |
Alpha-pinene and Beta-pinene synthases were purified using His SpinTrap Ni-affinity columns and were assayed in vitro with GPP. Using gas chromatography-mass spectrometry (GCMS), we confirmed the synthesis of alpha and beta pinene monoterpenes from our enzyme assays. | Alpha-pinene and Beta-pinene synthases were purified using His SpinTrap Ni-affinity columns and were assayed in vitro with GPP. Using gas chromatography-mass spectrometry (GCMS), we confirmed the synthesis of alpha and beta pinene monoterpenes from our enzyme assays. | ||
| Line 20: | Line 20: | ||
<b></b> | <b></b> | ||
| - | <font size=" | + | <font size="2"><h3>2. Diterpene production in yeast</h3></font> |
<b></b> | <b></b> | ||
| - | <font size=" | + | <font size="2"><h3>3. Mountain Pine Beetle & Yeast Co-culture</h3></font> |
To prove that release methods by incubation of Mountain Pine Beetles | To prove that release methods by incubation of Mountain Pine Beetles | ||
<b></b> | <b></b> | ||
| - | <font size=" | + | <font size="2"><h3>3. Blue Stain Fungi & Yeast Co-culture Co-culture</h3></font> |
| - | <font size=" | + | <font size="3"><h3>Modeling Achievements</h3></font> |
<hr style="color:#BDCBBD; height:3px;" /> | <hr style="color:#BDCBBD; height:3px;" /> | ||
| Line 48: | Line 48: | ||
<div id="links"></center> | <div id="links"></center> | ||
| - | <font size=" | + | <font size="3"><h3>Human Practices Achievements</h3></font> |
<hr style="color:#BDCBBD; height:3px;" /> | <hr style="color:#BDCBBD; height:3px;" /> | ||
| - | <font size=" | + | <font size="3"><h3>Special Considerations</h3></font> |
<hr style="color:#BDCBBD; height:3px;" /> | <hr style="color:#BDCBBD; height:3px;" /> | ||
Revision as of 02:36, 28 October 2011
Contents |
Wet Laboratory Achievements
1. In vitro assay production of monoterpene in bacteria
Geranyl Pyrophosphate (GPP) Assay
Alpha-pinene and Beta-pinene synthases were purified using His SpinTrap Ni-affinity columns and were assayed in vitro with GPP. Using gas chromatography-mass spectrometry (GCMS), we confirmed the synthesis of alpha and beta pinene monoterpenes from our enzyme assays.
2. Diterpene production in yeast
3. Mountain Pine Beetle & Yeast Co-culture
To prove that release methods by incubation of Mountain Pine Beetles
3. Blue Stain Fungi & Yeast Co-culture Co-culture
Modeling Achievements
Monoterpene Production
We simulated the expansion of the MPB population from year 2011 to 2020 using the estimates obtained from the clustering analysis. For cost estimation and prediction of emergence of subpopulations, refer to our Model Methodology above.
