"
Team:British Columbia/Accomplishments
From 2011.igem.org
(Difference between revisions)
| Line 12: | Line 12: | ||
<br> | <br> | ||
| - | Alpha-beta pinene and Beta-pinene synthases were | + | |
| + | Alpha-beta pinene and Beta-pinene synthases were purified using His SpinTrap Ni-affinity columns and were assayed in vitro with GPP. Using gas chromatography-mass spectrometry (GCMS) confirm the synthesis of alpha and beta pinene monoterpene. | ||
Revision as of 01:19, 28 October 2011
Wet Laboratory Achievements
Contents |
1. In vitro assay production of monoterpene in bacteria
Geranyl Pyrophosphate (GPP) Assay
Alpha-beta pinene and Beta-pinene synthases were purified using His SpinTrap Ni-affinity columns and were assayed in vitro with GPP. Using gas chromatography-mass spectrometry (GCMS) confirm the synthesis of alpha and beta pinene monoterpene.
2. Diterpene production in yeast
3. Mountain Pine Beetle & Yeast Co-culture
3. Blue Stain Fungi & Yeast Co-culture
Modeling Achievements
Monoterpene Production
We simulated the expansion of the MPB population from year 2011 to 2020 using the estimates obtained from the clustering analysis. For cost estimation and prediction of emergence of subpopulations, refer to our Model Methodology above.
Monoterpene Production
We simulated the expansion of the MPB population from year 2011 to 2020 using the estimates obtained from the clustering analysis. For cost estimation and prediction of emergence of subpopulations, refer to our Model Methodology above.
Human Practice
Special Considerations
