Team:Grinnell/Notebook/Gels

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Grinnell Menubar

Gel Pictures

June 6 to 10

PCR products on DNA gel. Lane 1: ladder; Lane 2: rsaA from liquid culture Caulobacter; Lane 3: rsaA from plate culture Caulobacter; Lane 4: esp from S. epidermidis.

Gel results for transformation of ligations. Lane 1: ladder; Lane 2: digested plasmid with rsaA C-terminal; Lane 3: digested plasmid with rsaA C-terminal and esp; Lanes 4-8: digested plasmids from various colonies with esp.

June 13 to 17

Gel 1 of 2. Lane 1: ladder; lanes 2-4: PCR product using VF2 and VR of plasmid containing esp ligated with rsaA C-terminal that had been digested 20110606; lane 5: PCR product of plasmid containing esp; lanes 6-8: PCR product using VF2 and VR of plasmid containing esp ligated with rsaA C-terminal PCR product from 20110605. Only lane 6 shows successful ligation of esp and rsaA C-terminal.

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 ===June 6 to 10===
 <gallery>
-<html><a name="1"></a></html>File:20110606_PCRproduct.jpg|PCR products on DNA gel. Lane 1: ladder; Lane 2: ''rsaA'' from liquid culture ''Caulobacter''; Lane 3: ''rsaA'' from plate culture ''Caulobacter''; Lane 4: ''esp'' from ''S. epidermidis''.
+File:20110606_PCRproduct.jpg|<html><a name="1"></a></html>PCR products on DNA gel. Lane 1: ladder; Lane 2: ''rsaA'' from liquid culture ''Caulobacter''; Lane 3: ''rsaA'' from plate culture ''Caulobacter''; Lane 4: ''esp'' from ''S. epidermidis''.
-<html><a name="2"></a></html>File:20110610PlamidGel.jpg|Gel results for transformation of ligations. Lane 1: ladder; Lane 2: digested plasmid with ''rsaA'' C-terminal; Lane 3: digested plasmid with ''rsaA'' C-terminal and ''esp''; Lanes 4-8: digested plasmids from various colonies with ''esp''.
+File:20110610PlamidGel.jpg|Gel results for transformation of ligations. Lane 1: ladder; Lane 2: digested plasmid with ''rsaA'' C-terminal; Lane 3: digested plasmid with ''rsaA'' C-terminal and ''esp''; Lanes 4-8: digested plasmids from various colonies with ''esp''.
 </gallery>
 ===June 13 to 17===
 <gallery>
-<html><a name=""></a></html>File:20110614_PCR135.jpg|Gel 1 of 2. Lane 1: ladder; lanes 2-4: PCR product using VF2 and VR of plasmid containing ''esp'' ligated with ''rsaA'' C-terminal that had been digested 20110606; lane 5: PCR product of plasmid containing ''esp''; lanes 6-8: PCR product using VF2 and VR of plasmid containing ''esp'' ligated with ''rsaA'' C-terminal PCR product from 20110605. Only lane 6 shows successful ligation of ''esp'' and ''rsaA'' C-terminal.
+File:20110614_PCR135.jpg|Gel 1 of 2. Lane 1: ladder; lanes 2-4: PCR product using VF2 and VR of plasmid containing ''esp'' ligated with ''rsaA'' C-terminal that had been digested 20110606; lane 5: PCR product of plasmid containing ''esp''; lanes 6-8: PCR product using VF2 and VR of plasmid containing ''esp'' ligated with ''rsaA'' C-terminal PCR product from 20110605. Only lane 6 shows successful ligation of ''esp'' and ''rsaA'' C-terminal.
 <html><a name=""></a></html>File:20110614_PCR246.jpg|Gel 2 of 2.  Lane 1: ladder; lanes 2-4: PCR product using VF2 and VR of plasmid containing ''esp'' ligated with ''rsaA'' C-terminal that had been digested 20110606; lane 5: PCR product of plasmid containing ''esp''; lanes 6-8: PCR product using VF2 and VR of plasmid containing ''esp'' ligated with ''rsaA'' C-terminal PCR product from 20110605.  None of these show successful ligation.
 <html><a name=""></a></html>File:20110614_Promoters.jpg|Gel of PCR of ''Caulobacter'' promoters Pxyl (inducible) and PrsaA (constitutive). Lane 1: ladder; lane 2: PrsaA; Lane 3: Pxyl. Bands appear, but are hazy and spread out due to the small size of DNA fragments.