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Team:Dundee/Safety
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<h2>Researcher Safety</h2> | <h2>Researcher Safety</h2> | ||
| - | <p>We attended a general health & safety induction and were given a safety tour of our lab involving guidance in waste disposal of sharps, trace chemicals, and biohazardous material.</ | + | <p>We attended a general health & safety induction and were given a safety tour of our lab involving guidance in waste disposal of sharps, trace chemicals, and biohazardous material.</p> |
<p>As a risk reduction measure we opted to use Qiagen kits rather than phenol based protocols.</p> | <p>As a risk reduction measure we opted to use Qiagen kits rather than phenol based protocols.</p> | ||
<p>Ethidium bromide is an intercalating agent(inserts into the DNA helix) in common use in laboratories as a means of detecting nucleic acids through agarose gel electrophoresis. As ethidiumbromide distorts the structure of the DNA helix, it is a mutagen and carcinogen.To avoid the risk of exposure to ethidium bromide, we decided instead to use GelRed stain in our agarose.</p> | <p>Ethidium bromide is an intercalating agent(inserts into the DNA helix) in common use in laboratories as a means of detecting nucleic acids through agarose gel electrophoresis. As ethidiumbromide distorts the structure of the DNA helix, it is a mutagen and carcinogen.To avoid the risk of exposure to ethidium bromide, we decided instead to use GelRed stain in our agarose.</p> | ||
Revision as of 14:33, 1 July 2011
The University of Dundee
iGem 2011
Researcher Safety
We attended a general health & safety induction and were given a safety tour of our lab involving guidance in waste disposal of sharps, trace chemicals, and biohazardous material.
As a risk reduction measure we opted to use Qiagen kits rather than phenol based protocols.
Ethidium bromide is an intercalating agent(inserts into the DNA helix) in common use in laboratories as a means of detecting nucleic acids through agarose gel electrophoresis. As ethidiumbromide distorts the structure of the DNA helix, it is a mutagen and carcinogen.To avoid the risk of exposure to ethidium bromide, we decided instead to use GelRed stain in our agarose.
E.coli MG1655 is a disabled K12 strain which is non-pathogenic and is used for research purposes only. Salmonella is an LT2a strain which is attenuated making it non-pathogenic. This strain is allowed to be handled as a class 1 pathogen rather than class 2 so is not categorized as a biohazard.
While working in the lab, we are supervised by our instructors, advisors or lab technicians from the University’s School of Life Sciences Learning & Teaching staff.
