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Team:Lyon-INSA-ENS/Realisation/Week17
From 2011.igem.org
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<FONT COLOR="red"> <b>Strain construction</b> </FONT> <br/><br/> | <FONT COLOR="red"> <b>Strain construction</b> </FONT> <br/><br/> | ||
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PHL1414/pIG16 --> 5 clones<br/> | PHL1414/pIG16 --> 5 clones<br/> | ||
| - | A 5ml liquid culture of each clone obtened is started. | + | A 5ml liquid culture of each clone obtened is started.<br/> |
| + | Extraction and digestion by E and P.<br/> | ||
| + | The gel electrophoresis shows that the clones of MC4100/pIG2, MC4100/pIG6, PHL1414/pI16, PHL1414/pIG6 and A350/pIG2 are ok. There is nothing on the digested A350/pIG6 track.<br> | ||
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<FONT COLOR="blue"> <b>Strain collection</b> </FONT> | <FONT COLOR="blue"> <b>Strain collection</b> </FONT> | ||
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S = A350/pIG6<br/> | S = A350/pIG6<br/> | ||
Revision as of 16:10, 27 October 2011
Week 17
From Monday the 10th of October to Sunday the 16th of October 2011
Monday
Strain construction
We're back to the lab for few more experiments, so we're building up again the strain we'll need for these experiments.
Transformations : PHL1414/pIG2, PHL1414/pIG6, PHL1414/pIG16, MC4100/p85, MC4100/pIG2, MC4100/pIG6
Note:There was less than 2µl of p85.
Dickeya strain A350 was transformed by electroporation with pIG6 and pIG2. A 5ml of LB liquid culture of 1 clone of both strain is started.
Tuesday
Strain construction
Transformations results:
MC4100/pIG6 --> 1 clone
MC4100/pIG2 --> 2 clones
MC4100/p85 --> 0 clone
PHL1414/pIG2 --> 0 clone
PHL1414/pIG16 --> 5 clones
A 5ml liquid culture of each clone obtened is started.
Extraction and digestion by E and P.
The gel electrophoresis shows that the clones of MC4100/pIG2, MC4100/pIG6, PHL1414/pI16, PHL1414/pIG6 and A350/pIG2 are ok. There is nothing on the digested A350/pIG6 track.
Strain collection
S = A350/pIG6
S = A350/pIG2
The final concentration of glycerol is 40%. (500µl of culture + 500µl of Y80%)
