Team:UEA-JIC Norwich/Methods
From 2011.igem.org
Benjevans1 (Talk | contribs) |
Benjevans1 (Talk | contribs) |
||
Line 1: | Line 1: | ||
- | <div style="background-color:# | + | <div style="background-color:#43BFC7; color:#254117"> |
+ | |||
+ | |||
<html> | <html> | ||
Line 66: | Line 68: | ||
<ul class="drop-down"> | <ul class="drop-down"> | ||
<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Project">Overview</a></li> | <li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Project">Overview</a></li> | ||
- | <li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Nittygritty"> | + | <li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Nittygritty-bacteria">Bacteria</a></li> |
- | + | <li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Nittygritty-algae">Algae</a></li> | |
+ | <li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Nittygritty-moss">Moss</a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
Line 92: | Line 95: | ||
<li class="top-li"><a class="top-a" href="https://2011.igem.org/Team:UEA-JIC_Norwich/Safety"><b>Lab Safety</b></a> | <li class="top-li"><a class="top-a" href="https://2011.igem.org/Team:UEA-JIC_Norwich/Safety"><b>Lab Safety</b></a> | ||
</li> | </li> | ||
- | + | ||
- | + | ||
<li class="top-li"><a class="top-a down" href=""><b>Human Practices</b></a> | <li class="top-li"><a class="top-a down" href=""><b>Human Practices</b></a> | ||
<ul class="drop-down"> | <ul class="drop-down"> | ||
Line 99: | Line 101: | ||
<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Energyconservation">Energy Conservation</a></li> | <li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Energyconservation">Energy Conservation</a></li> | ||
</ul></li> | </ul></li> | ||
- | </div> | + | <li class="top-li"><a class="top-a" href="https://2011.igem.org/Team:UEA-JIC_Norwich/Media"><b>Media</b></a> |
+ | </li> | ||
+ | <li class="top-li"><a class="top-a" href="https://2011.igem.org/Team:UEA-JIC_Norwich/Follow_us"><b>Follow Us</b></a> | ||
+ | </li> | ||
+ | |||
+ | </div> | ||
<html> | <html> | ||
<div id="contentgrid"> | <div id="contentgrid"> | ||
</html> | </html> | ||
- | |||
<html> | <html> | ||
<head> | <head> |
Revision as of 09:34, 1 July 2011
High Efficiency Transformation Protocol
1. Thaw a tube of NEB 5-alpha Competent E.coli cells on ice for 10 minutes
2. Mark the location of the Biobrick well (letters from top to bottom, numbers from left to right)
3. Resuspend specific biobrick part (1 μl) with distilled water (20 μl). Aspirate up and down a few times
4. Inoculate with DNA ( 1 μl) to the competent cells
5. Place the mixture on ice for 30 minutes. Do not mix
6. Heat shock at exactly 42°C for exactly 30 seconds. Do not mix
7. Place on ice for 5 minutes. Do not mix
8. Pipette 950 μl of room temperature SOC into the mixture
9. Place at 37°C for 60 minutes. Shake vigorously (250 rpm) or rotate
10. Warm selection plates to 37°C
11. Do serial dilutions (2-3) at 105. Mix cells, flick/invert gently
12. Spread (100 μl) on agar plates of each dilution
13. Incubate overnight at 37°C