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| - | {{:Team:Tsinghua-A/template/killbanner}}
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| - | <html xmlns="http://www.w3.org/1999/xhtml">
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| - | <head>
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| - | <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" />
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| - | <title>JavaScript Slideshow - TinySlideshow</title>
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| - | <link rel="stylesheet" href="http://bioinfo.au.tsinghua.edu.cn/member/IGEM/thu_style.css" />
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| - | <style>
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| - | body {
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| - | margin: 0;
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| - | background: #F7F7F7 url("https://static.igem.org/mediawiki/2011/2/27/Thua-back1.jpg");
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| - | background-repeat: no-repeat;
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| - | background-attachment: fixed;
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| - | font-family: Arial, Helvetica, sans-serif;
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| - | font-size: 12px;
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| - | color: #787878;
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| - | }
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| - | head{
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| - | margin:0;
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| - | padding:0;
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| - | //background: #F7F7F7 url("https://static.igem.org/mediawiki/2011/e/e2/Thua-back.jpg") repeat-x left top;
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| - | }
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| - | p{
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| - | text-indent:2em;
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| - | line-height:2;
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| - | font-family:"Times New Roman",Georgia,Serif;
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| - | color:#000000;
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| - | font-size:110%
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| - | }
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| - | h1 {
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| - | font-family:"Times New Roman",Georgia,Serif;
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| - | color:#548dd4;
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| - | font-weight:bold;
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| - | font-size:130%;
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| - | padding:0;
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| - | border:none;
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| - | margin:0;
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| - | }
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| - | /* Menu */
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| - |
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| - | #menu {
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| - | width: 940px;
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| - | height: 49px;
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| - | margin: 0 auto;
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| - | padding: 0;
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| - | background: #1c1c1c;
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| - | }
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| - | #menu ul {
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| - | list-style: none;
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| - | line-height: normal;
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| - | }
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| - | #menu li {
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| - | float: left;
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| - | border-left: 1px solid #FFFFFF;
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| - | }
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| - | #menu a {
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| - | display: block;
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| - | width: 155px;
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| - | height: 33px;
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| - | padding-top: 16px;
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| - | text-decoration: none;
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| - | text-align: center;
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| - | font-family: Arial, Helvetica, sans-serif;
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| - | font-size: 13px;
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| - | font-weight: bold;
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| - | color: #f7f7f7;
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| - | border: none;
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| - | }
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| - | #menu a:hover, #menu .current_page_item a {
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| - | background: #727272;
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| - | text-decoration: none;
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| - | }
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| - | #menu .current_page_item a {
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| - | background: #727272;
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| - | padding-left: 0;
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| - | }
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| - | #menu .normal_item a {
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| - | background: #1c1c1c;
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| - | padding-left: 0;
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| - | }
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| - | </style>
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| - |
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| - |
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| - | </head>
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| - | <body>
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| - | <p align="CENTER" STYLE="text-indent:0em"><img src="https://static.igem.org/mediawiki/2011/1/17/THU_banner_par.jpg" alt="" width="960"/></p>
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| - | <div id="menu">
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| - | <ul>
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| - | <li><a href="https://2011.igem.org/Team:Tsinghua-A/Team">Team</a></li>
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| - | <li><a href="https://2011.igem.org/Team:Tsinghua-A/Project">Project</a></li>
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| - | <li><a href="https://2011.igem.org/Team:Tsinghua-A/Safety">Safety</a></li>
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| - | <li ><a href="https://2011.igem.org/Team:Tsinghua-A/Modeling">Modeling</a></li>
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| - | <li class="current_page_item"><a href="https://2011.igem.org/Team:Tsinghua-A/Parts">Parts</a></li>
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| - | <li ><a href="https://2011.igem.org/Team:Tsinghua-A/Notebook">Notebook</a></li>
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| - | </ul>
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| - | </div>
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| - |
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| - | <!--end of enu--------------------------------------------><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN">
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| - | <HTML>
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| - | <HEAD>
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| - | <META HTTP-EQUIV="CONTENT-TYPE" CONTENT="text/html; charset=gb2312">
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| - | <TITLE></TITLE>
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| - | <META NAME="GENERATOR" CONTENT="OpenOffice.org 3.2 (Win32)">
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| - | <META NAME="AUTHOR" CONTENT="Sl Y">
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| - | <META NAME="CREATED" CONTENT="20111002;3132500">
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| - | <META NAME="CHANGEDBY" CONTENT="Sl Y">
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| - | <META NAME="CHANGED" CONTENT="20111002;20522400">
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| - | <META NAME="CHANGEDBY" CONTENT="Sl Y">
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| - | </HEAD>
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| - | <BODY LANG="zh-CN" DIR="LTR">
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| - | <br>
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| - |
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| - | <h1>Parts List</h1><hr width="100%" size=2 color=gray>
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| - | <br>
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| - | <TABLE Align=CENTER WIDTH=760 BORDER=10 CELLPADDING=0 CELLSPACING=0 BGCOLOR="#ccccff">
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| - | <COL WIDTH=160>
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| - | <COL WIDTH=130>
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| - | <COL WIDTH=340>
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| - | <COL WIDTH=130>
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| - | <TR>
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| - | <TH WIDTH=160 BGCOLOR="#aaaaff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Name</SPAN></FONT></FONT></P>
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| - | </TH>
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| - | <TH WIDTH=130 BGCOLOR="#aaaaff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Type</SPAN></FONT></FONT></P>
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| - | </TH>
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| - | <TH WIDTH=340 BGCOLOR="#aaaaff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Description</SPAN></FONT></FONT></P>
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| - | </TH>
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| - | <TH WIDTH=130 BGCOLOR="#aaaaff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Length</SPAN></FONT></FONT></P>
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| - | </TH>
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| - | </TR>
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| - | <TR>
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| - | <TD WIDTH=160 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574000"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574000</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
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| - | </TD>
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| - | <TD WIDTH=130 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
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| - | </TD>
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| - | <TD WIDTH=340 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">pLux + TetR</SPAN></FONT></FONT></P>
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| - | </TD>
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| - | <TD WIDTH=130 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">903</SPAN></FONT></FONT></P>
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| - | </TD>
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| - | </TR>
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| - | <TR>
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| - | <TD WIDTH=160 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574001"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574001</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
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| - | </TD>
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| - | <TD WIDTH=130 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
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| - | </TD>
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| - | <TD WIDTH=340 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">TetR
| |
| - | regulated by 3OC6HSL</SPAN></FONT></FONT></P>
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| - | </TD>
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| - | <TD WIDTH=130 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">1890</SPAN></FONT></FONT></P>
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| - | </TD>
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| - | </TR>
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| - | <TR>
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| - | <TD WIDTH=160 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574002"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574002</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
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| - | </TD>
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| - | <TD WIDTH=130 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
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| - | </TD>
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| - | <TD WIDTH=340 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">pluxR
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| - | + CFP</SPAN></FONT></FONT></P>
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| - | </TD>
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| - | <TD WIDTH=130 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">941</SPAN></FONT></FONT></P>
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| - | </TD>
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| - | </TR>
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| - | <TR>
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| - | <TD WIDTH=160 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574003"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574003</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
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| - | </TD>
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| - | <TD WIDTH=130 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Device</SPAN></FONT></FONT></P>
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| - | </TD>
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| - | <TD WIDTH=340 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC6HSL -> TetR && reporter</SPAN></FONT></FONT></P>
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| - | </TD>
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| - | <TD WIDTH=130 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">2839</SPAN></FONT></FONT></P>
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| - | </TD>
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| - | </TR>
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| - | <TR>
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| - | <TD WIDTH=160 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574004"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574004</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
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| - | </TD>
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| - | <TD WIDTH=130 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=340 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC12HSL regulated by TetR</SPAN></FONT></FONT></P>
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| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">797</SPAN></FONT></FONT></P>
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| - | </TD>
| |
| - | </TR>
| |
| - | <TR>
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| - | <TD WIDTH=160 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574005"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574005</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
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| - | </TD>
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| - | <TD WIDTH=130 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=340 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC12HSL and YFP regulated by pBad</SPAN></FONT></FONT></P>
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| - | </TD>
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| - | <TD WIDTH=130 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">1712</SPAN></FONT></FONT></P>
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| - | </TD>
| |
| - | </TR>
| |
| - | <TR>
| |
| - | <TD WIDTH=160 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574006"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574006</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
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| - | </TD>
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| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Device</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=340 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC12HSL regulated by pTet and pBad</SPAN></FONT></FONT></P>
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| - | </TD>
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| - | <TD WIDTH=130 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">2517</SPAN></FONT></FONT></P>
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| - | </TD>
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| - | </TR>
| |
| - | <TR>
| |
| - | <TD WIDTH=160 BGCOLOR="#ddddff">
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| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574007"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574007</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
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| - | </TD>
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| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
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| - | </TD>
| |
| - | <TD WIDTH=340 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">lasR + TT</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">918</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | </TR>
| |
| - | <TR>
| |
| - | <TD WIDTH=160 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574008"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574008</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=340 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">a constitutive LasR producer</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">982</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | </TR>
| |
| - | <TR>
| |
| - | <TD WIDTH=160 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574009"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574009</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=340 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC12HSL -> PoPS Receiver</SPAN></FONT></FONT></P>
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| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">1147</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | </TR>
| |
| - | </TABLE>
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| - | <BR>
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| - | <h1>Descriptions</h1><hr width="100%" size=2 color=gray>
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| - | <br>
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| - | <p>We assembled <A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_E0840"><FONT COLOR="#002bb8">BBa_E0840</FONT></A>
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| - | under the pLas promoter
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| - | <A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_R0079"><FONT COLOR="#002bb8">BBa_R0079</FONT></A>
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| - | that was contained into K574009. We kept pSB1A2 as the scaffold vector.</p>
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| - | <br>
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| - | <h1>Methods</h1><hr width="100%" size=2 color=gray>
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| - | <br>
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| - | <p>1. Ligate
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| - | <A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_E0840"><FONT COLOR="#002bb8">BBa_E0840</FONT></A>
| |
| - | downstream of
| |
| - | <A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_K574009"><FONT COLOR="#002bb8">BBa_K574009</FONT></A>
| |
| - | , which acts as a reporter.</p>
| |
| - |
| |
| - | <p>2. Culture the positive colony at 37°C,
| |
| - | 220 rpm for 12 hours, as well as the DH5alpha.</p>
| |
| - |
| |
| - | <p>3. Dilute 1:25 the former in 15 falcon tubes (4 ml cultures),
| |
| - | each 3 tubes as a group. The later is also diluted in 3 tubes as
| |
| - | the negative control.</p>
| |
| - |
| |
| - | <p>4. After culturing for another 2 hours, as their OD600 reach 0.2, induce
| |
| - | with 3OC12HSL 0, 1e-7 M, 1e-6 M, 1e-5 M and 1e-4 M respectively.</p>
| |
| - |
| |
| - | <p>5. Take samples after 1 hour, 4 hours, 8 hours and 24 hours, that is,
| |
| - | centrifugalizate 0.5 mL of each culture and suspend them by 1 mL of PBS
| |
| - | (phosphate-buffered saline).</p>
| |
| - |
| |
| - | <P>6. Measure the fluorescence of the samples with the flow cytometer.</P>
| |
| - | <br>
| |
| - | <h1>Results</h1><hr width="100%" size=2 color=gray>
| |
| - | <br>
| |
| - | <p style="text-indent:0em"align="CENTER"><img src="https://static.igem.org/mediawiki/2011/c/ca/K574009_chart.png" NAME="K574009_chart" /></p>
| |
| - | <P>From
| |
| - | the chart, we can see that cells were hardly induced in the control
| |
| - | group, and with the concentration of inducer growing, the intensity
| |
| - | of GFP increased by groups. The most efficient concentration of
| |
| - | inducer was around 10<sup>-5</sup>M, and higher concentration may lead to the
| |
| - | expression of GFP decreasing. Additionally, to most groups, the intensity of GFP
| |
| - | reached its maxium after 4 hours.</P>
| |
| - |
| |
| - |
| |
| - | <br><br><br>
| |
| - | <p align="CENTER" STYLE="text-indent:0em"><a href="https://2011.igem.org/Team:Tsinghua-A"><img src="https://static.igem.org/mediawiki/2011/9/92/Killbanner_header.jpg" alt="" width="960"/><a href="https://2011.igem.org"><img src="https://static.igem.org/mediawiki/igem.org/2/29/Killbanner_header2.jpg" alt="" width="960"/></p>
| |
| - | </BODY>
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| - | </HTML>
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| - | {{:Team:Tsinghua-A/template/killbanner}}
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| |
| - | <head>
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| - | <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" />
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| - | <title>JavaScript Slideshow - TinySlideshow</title>
| |
| - | <link rel="stylesheet" href="http://bioinfo.au.tsinghua.edu.cn/member/IGEM/thu_style.css" />
| |
| - | <style>
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| - |
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| - |
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| - | body {
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| - | margin: 0;
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| - | background: #F7F7F7 url("https://static.igem.org/mediawiki/2011/2/27/Thua-back1.jpg");
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| - | //background: #F7F7F7 url("https://static.igem.org/mediawiki/2011/e/e2/Thua-back.jpg") repeat-x left top;
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| - | }
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| - | p{
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| - | font-size:110%
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| - | /* Menu */
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| - | list-style: none;
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| - | line-height: normal;
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| - | }
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| - |
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| - | #menu li {
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| - | float: left;
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| - | border-left: 1px solid #FFFFFF;
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| - | }
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| - |
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| - | #menu a {
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| - | display: block;
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| - | width: 155px;
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| - | height: 33px;
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| - | font-weight: bold;
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| - | color: #f7f7f7;
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| - | #menu a:hover, #menu .current_page_item a {
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| - | background: #727272;
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| - | text-decoration: none;
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| - | #menu .current_page_item a {
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| - | }
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| - | #menu .normal_item a {
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| - | }
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| - | </style>
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| - |
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| - | </head>
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| - | <body>
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| - | <p align="CENTER" STYLE="text-indent:0em"><img src="https://static.igem.org/mediawiki/2011/1/17/THU_banner_par.jpg" alt="" width="960"/></p>
| |
| - | <div id="menu">
| |
| - | <ul>
| |
| - | <li><a href="https://2011.igem.org/Team:Tsinghua-A/Team">Team</a></li>
| |
| - | <li><a href="https://2011.igem.org/Team:Tsinghua-A/Project">Project</a></li>
| |
| - | <li><a href="https://2011.igem.org/Team:Tsinghua-A/Safety">Safety</a></li>
| |
| - | <li ><a href="https://2011.igem.org/Team:Tsinghua-A/Modeling">Modeling</a></li>
| |
| - | <li class="current_page_item"><a href="https://2011.igem.org/Team:Tsinghua-A/Parts">Parts</a></li>
| |
| - | <li ><a href="https://2011.igem.org/Team:Tsinghua-A/Notebook">Notebook</a></li>
| |
| - | </ul>
| |
| - | </div>
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| - |
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| - | <!--end of enu--------------------------------------------><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN">
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| - | <HTML>
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| - | <HEAD>
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| - | <META HTTP-EQUIV="CONTENT-TYPE" CONTENT="text/html; charset=gb2312">
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| - | <TITLE></TITLE>
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| - | <META NAME="GENERATOR" CONTENT="OpenOffice.org 3.2 (Win32)">
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| - | <META NAME="AUTHOR" CONTENT="Sl Y">
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| - | <META NAME="CREATED" CONTENT="20111002;3132500">
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| - | <META NAME="CHANGEDBY" CONTENT="Sl Y">
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| - | <META NAME="CHANGED" CONTENT="20111002;20522400">
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| - | <META NAME="CHANGEDBY" CONTENT="Sl Y">
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| - | </HEAD>
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| - | <BODY LANG="zh-CN" DIR="LTR">
| |
| - | <br>
| |
| - |
| |
| - | <h1>Parts List</h1><hr width="100%" size=2 color=gray>
| |
| - | <br>
| |
| - | <TABLE Align=CENTER WIDTH=760 BORDER=5 CELLPADDING=0 CELLSPACING=0 BGCOLOR="#ccccff">
| |
| - | <COL WIDTH=160>
| |
| - | <COL WIDTH=130>
| |
| - | <COL WIDTH=340>
| |
| - | <COL WIDTH=130>
| |
| - | <TR>
| |
| - | <TH WIDTH=160 BGCOLOR="#aaaaff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Name</SPAN></FONT></FONT></P>
| |
| - | </TH>
| |
| - | <TH WIDTH=130 BGCOLOR="#aaaaff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Type</SPAN></FONT></FONT></P>
| |
| - | </TH>
| |
| - | <TH WIDTH=340 BGCOLOR="#aaaaff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Description</SPAN></FONT></FONT></P>
| |
| - | </TH>
| |
| - | <TH WIDTH=130 BGCOLOR="#aaaaff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Length</SPAN></FONT></FONT></P>
| |
| - | </TH>
| |
| - | </TR>
| |
| - | <TR>
| |
| - | <TD WIDTH=160 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574000"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574000</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=340 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">pLux + TetR</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">903</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | </TR>
| |
| - | <TR>
| |
| - | <TD WIDTH=160 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574001"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574001</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=340 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">TetR
| |
| - | regulated by 3OC6HSL</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">1890</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | </TR>
| |
| - | <TR>
| |
| - | <TD WIDTH=160 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574002"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574002</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=340 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">pluxR
| |
| - | + CFP</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">941</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | </TR>
| |
| - | <TR>
| |
| - | <TD WIDTH=160 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574003"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574003</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Device</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=340 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC6HSL -> TetR && reporter</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">2839</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | </TR>
| |
| - | <TR>
| |
| - | <TD WIDTH=160 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574004"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574004</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=340 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC12HSL regulated by TetR</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">797</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | </TR>
| |
| - | <TR>
| |
| - | <TD WIDTH=160 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574005"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574005</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=340 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC12HSL and YFP regulated by pBad</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">1712</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | </TR>
| |
| - | <TR>
| |
| - | <TD WIDTH=160 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574006"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574006</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Device</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=340 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC12HSL regulated by pTet and pBad</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">2517</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | </TR>
| |
| - | <TR>
| |
| - | <TD WIDTH=160 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574007"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574007</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=340 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">lasR + TT</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">918</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | </TR>
| |
| - | <TR>
| |
| - | <TD WIDTH=160 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574008"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574008</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=340 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">a constitutive LasR producer</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">982</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | </TR>
| |
| - | <TR>
| |
| - | <TD WIDTH=160 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574009"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574009</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=340 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC12HSL -> PoPS Receiver</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | <TD WIDTH=130 BGCOLOR="#ddddff">
| |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">1147</SPAN></FONT></FONT></P>
| |
| - | </TD>
| |
| - | </TR>
| |
| - | </TABLE>
| |
| - | <BR>
| |
| - | <h1>Descriptions</h1><hr width="100%" size=2 color=gray>
| |
| - | <br>
| |
| - | <p>We assembled <A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_E0840"><FONT COLOR="#002bb8">BBa_E0840</FONT></A>
| |
| - | under the pLas promoter
| |
| - | <A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_R0079"><FONT COLOR="#002bb8">BBa_R0079</FONT></A>
| |
| - | that was contained into K574009. We kept pSB1A2 as the scaffold vector.</p>
| |
| - | <br>
| |
| - | <h1>Methods</h1><hr width="100%" size=2 color=gray>
| |
| - | <br>
| |
| - | <p>1. Ligate
| |
| - | <A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_E0840"><FONT COLOR="#002bb8">BBa_E0840</FONT></A>
| |
| - | downstream of
| |
| - | <A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_K574009"><FONT COLOR="#002bb8">BBa_K574009</FONT></A>
| |
| - | , which acts as a reporter.</p>
| |
| - |
| |
| - | <p>2. Culture the positive colony at 37°C,
| |
| - | 220 rpm for 12 hours, as well as the DH5alpha.</p>
| |
| - |
| |
| - | <p>3. Dilute 1:25 the former in 15 falcon tubes (4 ml cultures),
| |
| - | each 3 tubes as a group. The later is also diluted in 3 tubes as
| |
| - | the negative control.</p>
| |
| - |
| |
| - | <p>4. After culturing for another 2 hours, as their OD600 reach 0.2, induce
| |
| - | with 3OC12HSL 0, 1e-7 M, 1e-6 M, 1e-5 M and 1e-4 M respectively.</p>
| |
| - |
| |
| - | <p>5. Take samples after 1 hour, 4 hours, 8 hours and 24 hours, that is,
| |
| - | centrifugalizate 0.5 mL of each culture and suspend them by 1 mL of PBS
| |
| - | (phosphate-buffered saline).</p>
| |
| - |
| |
| - | <P>6. Measure the fluorescence of the samples with the flow cytometer.</P>
| |
| - | <br>
| |
| - | <h1>Results</h1><hr width="100%" size=2 color=gray>
| |
| - | <br>
| |
| - | <p style="text-indent:0em"align="CENTER"><img src="https://static.igem.org/mediawiki/2011/c/ca/K574009_chart.png" NAME="K574009_chart" /></p>
| |
| - | <P>From
| |
| - | the chart, we can see that cells were hardly induced in the control
| |
| - | group, and with the concentration of inducer growing, the intensity
| |
| - | of GFP increased by groups. The most efficient concentration of
| |
| - | inducer was around 10<sup>-5</sup>M, and higher concentration may lead to the
| |
| - | expression of GFP decreasing. Additionally, to most groups, the intensity of GFP
| |
| - | reached its maxium after 4 hours.</P>
| |
| - |
| |
| - |
| |
| - | <br><br><br>
| |
| - | <p align="CENTER" STYLE="text-indent:0em"><a href="https://2011.igem.org/Team:Tsinghua-A"><img src="https://static.igem.org/mediawiki/2011/9/92/Killbanner_header.jpg" alt="" width="960"/><a href="https://2011.igem.org"><img src="https://static.igem.org/mediawiki/igem.org/2/29/Killbanner_header2.jpg" alt="" width="960"/></p>
| |
| - | </BODY>
| |
| - | </HTML>
| |
| | {{:Team:Tsinghua-A/template/killbanner}} | | {{:Team:Tsinghua-A/template/killbanner}} |
| | <html xmlns="http://www.w3.org/1999/xhtml"> | | <html xmlns="http://www.w3.org/1999/xhtml"> |
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| | <TR> | | <TR> |
| | <TH WIDTH=160 BGCOLOR="#aaaaff"> | | <TH WIDTH=160 BGCOLOR="#aaaaff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Name</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Name</SPAN></FONT></FONT></P> |
| | </TH> | | </TH> |
| | <TH WIDTH=130 BGCOLOR="#aaaaff"> | | <TH WIDTH=130 BGCOLOR="#aaaaff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Type</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Type</SPAN></FONT></FONT></P> |
| | </TH> | | </TH> |
| | <TH WIDTH=340 BGCOLOR="#aaaaff"> | | <TH WIDTH=340 BGCOLOR="#aaaaff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Description</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Description</SPAN></FONT></FONT></P> |
| | </TH> | | </TH> |
| | <TH WIDTH=130 BGCOLOR="#aaaaff"> | | <TH WIDTH=130 BGCOLOR="#aaaaff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Length</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Length</SPAN></FONT></FONT></P> |
| | </TH> | | </TH> |
| | </TR> | | </TR> |
| | <TR> | | <TR> |
| | <TD WIDTH=160 BGCOLOR="#ddddff"> | | <TD WIDTH=160 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574000"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574000</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm ; text-indent:0em"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574000"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574000</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=130 BGCOLOR="#ddddff"> | | <TD WIDTH=130 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=340 BGCOLOR="#ddddff"> | | <TD WIDTH=340 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">pLux + TetR</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">pLux + TetR</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=130 BGCOLOR="#ddddff"> | | <TD WIDTH=130 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">903</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">903</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | </TR> | | </TR> |
| | <TR> | | <TR> |
| | <TD WIDTH=160 BGCOLOR="#ddddff"> | | <TD WIDTH=160 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574001"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574001</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm ; text-indent:0em"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574001"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574001</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=130 BGCOLOR="#ddddff"> | | <TD WIDTH=130 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=340 BGCOLOR="#ddddff"> | | <TD WIDTH=340 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">TetR | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">TetR |
| | regulated by 3OC6HSL</SPAN></FONT></FONT></P> | | regulated by 3OC6HSL</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=130 BGCOLOR="#ddddff"> | | <TD WIDTH=130 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">1890</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">1890</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | </TR> | | </TR> |
| | <TR> | | <TR> |
| | <TD WIDTH=160 BGCOLOR="#ddddff"> | | <TD WIDTH=160 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574002"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574002</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm ; text-indent:0em"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574002"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574002</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=130 BGCOLOR="#ddddff"> | | <TD WIDTH=130 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=340 BGCOLOR="#ddddff"> | | <TD WIDTH=340 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">pluxR | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">pluxR |
| | + CFP</SPAN></FONT></FONT></P> | | + CFP</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=130 BGCOLOR="#ddddff"> | | <TD WIDTH=130 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">941</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">941</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | </TR> | | </TR> |
| | <TR> | | <TR> |
| | <TD WIDTH=160 BGCOLOR="#ddddff"> | | <TD WIDTH=160 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574003"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574003</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm ; text-indent:0em"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574003"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574003</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=130 BGCOLOR="#ddddff"> | | <TD WIDTH=130 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Device</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Device</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=340 BGCOLOR="#ddddff"> | | <TD WIDTH=340 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC6HSL -> TetR && reporter</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC6HSL -> TetR && reporter</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=130 BGCOLOR="#ddddff"> | | <TD WIDTH=130 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">2839</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">2839</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | </TR> | | </TR> |
| | <TR> | | <TR> |
| | <TD WIDTH=160 BGCOLOR="#ddddff"> | | <TD WIDTH=160 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574004"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574004</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm ; text-indent:0em"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574004"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574004</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=130 BGCOLOR="#ddddff"> | | <TD WIDTH=130 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=340 BGCOLOR="#ddddff"> | | <TD WIDTH=340 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC12HSL regulated by TetR</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC12HSL regulated by TetR</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=130 BGCOLOR="#ddddff"> | | <TD WIDTH=130 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">797</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">797</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | </TR> | | </TR> |
| | <TR> | | <TR> |
| | <TD WIDTH=160 BGCOLOR="#ddddff"> | | <TD WIDTH=160 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574005"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574005</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm ; text-indent:0em"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574005"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574005</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=130 BGCOLOR="#ddddff"> | | <TD WIDTH=130 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=340 BGCOLOR="#ddddff"> | | <TD WIDTH=340 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC12HSL and YFP regulated by pBad</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC12HSL and YFP regulated by pBad</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=130 BGCOLOR="#ddddff"> | | <TD WIDTH=130 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">1712</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">1712</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | </TR> | | </TR> |
| | <TR> | | <TR> |
| | <TD WIDTH=160 BGCOLOR="#ddddff"> | | <TD WIDTH=160 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574006"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574006</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm ; text-indent:0em"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574006"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574006</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=130 BGCOLOR="#ddddff"> | | <TD WIDTH=130 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Device</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Device</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=340 BGCOLOR="#ddddff"> | | <TD WIDTH=340 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC12HSL regulated by pTet and pBad</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC12HSL regulated by pTet and pBad</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=130 BGCOLOR="#ddddff"> | | <TD WIDTH=130 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">2517</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">2517</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | </TR> | | </TR> |
| | <TR> | | <TR> |
| | <TD WIDTH=160 BGCOLOR="#ddddff"> | | <TD WIDTH=160 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574007"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574007</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm ; text-indent:0em"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574007"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574007</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=130 BGCOLOR="#ddddff"> | | <TD WIDTH=130 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=340 BGCOLOR="#ddddff"> | | <TD WIDTH=340 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">lasR + TT</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">lasR + TT</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=130 BGCOLOR="#ddddff"> | | <TD WIDTH=130 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">918</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">918</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | </TR> | | </TR> |
| | <TR> | | <TR> |
| | <TD WIDTH=160 BGCOLOR="#ddddff"> | | <TD WIDTH=160 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574008"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574008</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm ; text-indent:0em"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574008"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574008</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=130 BGCOLOR="#ddddff"> | | <TD WIDTH=130 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=340 BGCOLOR="#ddddff"> | | <TD WIDTH=340 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">a constitutive LasR producer</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">a constitutive LasR producer</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=130 BGCOLOR="#ddddff"> | | <TD WIDTH=130 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">982</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">982</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | </TR> | | </TR> |
| | <TR> | | <TR> |
| | <TD WIDTH=160 BGCOLOR="#ddddff"> | | <TD WIDTH=160 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574009"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574009</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm ; text-indent:0em"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574009"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574009</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=130 BGCOLOR="#ddddff"> | | <TD WIDTH=130 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=340 BGCOLOR="#ddddff"> | | <TD WIDTH=340 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC12HSL -> PoPS Receiver</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC12HSL -> PoPS Receiver</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | <TD WIDTH=130 BGCOLOR="#ddddff"> | | <TD WIDTH=130 BGCOLOR="#ddddff"> |
| - | <P ALIGN=CENTER STYLE="border: none; padding: 0cm text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">1147</SPAN></FONT></FONT></P> | + | <P ALIGN=CENTER STYLE="border: none; padding: 0cm; text-indent:0em"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">1147</SPAN></FONT></FONT></P> |
| | </TD> | | </TD> |
| | </TR> | | </TR> |
2. Culture the positive colony at 37°C,
220 rpm for 12 hours, as well as the DH5alpha.
3. Dilute 1:25 the former in 15 falcon tubes (4 ml cultures),
each 3 tubes as a group. The later is also diluted in 3 tubes as
the negative control.
4. After culturing for another 2 hours, as their OD600 reach 0.2, induce
with 3OC12HSL 0, 1e-7 M, 1e-6 M, 1e-5 M and 1e-4 M respectively.
5. Take samples after 1 hour, 4 hours, 8 hours and 24 hours, that is,
centrifugalizate 0.5 mL of each culture and suspend them by 1 mL of PBS
(phosphate-buffered saline).
6. Measure the fluorescence of the samples with the flow cytometer.
From
the chart, we can see that cells were hardly induced in the control
group, and with the concentration of inducer growing, the intensity
of GFP increased by groups. The most efficient concentration of
inducer was around 10-5M, and higher concentration may lead to the
expression of GFP decreasing. Additionally, to most groups, the intensity of GFP
reached its maxium after 4 hours.
"
