Team:Nevada/Notebook

From 2011.igem.org

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E. Coli
E. Coli
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Blah Blah Blah
Blah Blah Blah
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Reaction used Glucose assay kit (Genzyme) containing 2000U/L Hexokinase reagent and 4000U/L G-6-P DeH and measured glucose concentrations ranging from 0.05mM-0.25mM and absorbances were measured at 340.0 nm.
Reaction used Glucose assay kit (Genzyme) containing 2000U/L Hexokinase reagent and 4000U/L G-6-P DeH and measured glucose concentrations ranging from 0.05mM-0.25mM and absorbances were measured at 340.0 nm.
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Results: Final NADH concentrations were determined using the molar extinction coefficient and were proportionate to initial glucose concentrations.
Results: Final NADH concentrations were determined using the molar extinction coefficient and were proportionate to initial glucose concentrations.
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Revision as of 19:19, 30 June 2011


Week 1 - June 1-4
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E. Coli
Blah Blah Blah
Cyano
Blah Blah Blah
Hexokinase coupled assay used to quantitate amounts of cyanobacteria glucose secretion.
    Rxn: 1. glucose + ATP --Hx-> G-6-P + ADP
  • 2. G-6-P + NAD+ --G-6-PDeH-> G-6-P + NADH

Reaction used Glucose assay kit (Genzyme) containing 2000U/L Hexokinase reagent and 4000U/L G-6-P DeH and measured glucose concentrations ranging from 0.05mM-0.25mM and absorbances were measured at 340.0 nm.
Week 1                                                                                                                                                         Week 2

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