Team:ZJU-China/Project.html

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#REDIRECT [[Team:ZJU-China/Project]]
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<h4 class="current" id="try">Biofilm</h4>
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<div class="pane" style="display: block;"><a
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href="https://2011.igem.org/Team:ZJU-China/Project.html">Background</a> <a
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href="https://2011.igem.org/Team:ZJU-China/bio-Design.html">Design</a> <a
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href="https://2011.igem.org/Team:ZJU-China/bio-modeling.html">Modeling</a> <a
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href="https://2011.igem.org/Team:ZJU-China/bio-results.html">Results</a></div>
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<h4>Rainbofilm</h4>
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<div class="pane"><a
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href="https://2011.igem.org/Team:ZJU-China/rainbo-Background.html">Background</a>
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<a href="https://2011.igem.org/Team:ZJU-China/rainbo-Design.html">Design</a> <a
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href="https://2011.igem.org/Team:ZJU-China/rainbo-modeling-v1.html">Modeling</a> <a
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href="https://2011.igem.org/Team:ZJU-China/rainbo-Results.html">Results</a></div>
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<h4>Sugarfilm</h4>
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href="https://2011.igem.org/Team:ZJU-China/Sugar-background.html">Background</a> <a
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href="https://2011.igem.org/Team:ZJU-China/Sugar-Design.html">Design</a> <a
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href="https://2011.igem.org/Team:ZJU-China/Sugar-Modeling.html">Modeling</a> <a
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href="https://2011.igem.org/Team:ZJU-China/Sugar-Future.html">Future</a></div>
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<h4>Parts</h4>
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href="https://2011.igem.org/Team:ZJU-China/BBa_I13602.html">BBa_I13602</a> <a
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href="https://2011.igem.org/Team:ZJU-China/BBa_K561000.html">BBa_K561000</a> <a
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href="https://2011.igem.org/Team:ZJU-China/BBa_K561002.html">BBa_K561002</a> <a
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href="https://2011.igem.org/Team:ZJU-China/BBa_K561003.html">BBa_K561003</a></div>
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<h4>Achievements</h4>
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href="https://2011.igem.org/Team:ZJU-China/attribution.html">Attribution and
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Contributions</a></div>
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<h1>Background</h1>
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</div>
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<p></p>
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<p>Biofilm is a multi-cellular bacteria community, which is
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prevalent in diverse natural and artificial settings. It is best known
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for its robust adaptability and strong resistance to environmental
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stress. We intended to utilize and modify this natural multicellular
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structure to develop a new kind of extracellular scaffold, constructing
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a stratified protein expression system using the naturally formed graded
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oxygen concentration within the bacteria biofilm.<br />
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</p>
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<p></p>
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<p>Biofilm formation process can be roughly divided into four
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stages: bacteria attachment, micro colony formation, biofilm maturation
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and biofilm dispersion. The best sample for the observation is the
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matured biofilm, which, according to previous studies, was formed after
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48 hours after inoculation with only exception in bubbling method
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(Bandara HM et al. 2009; Klausen M. et al. 2003.). <br />
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</p>
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<p></p>
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<p>Based on the references (Almeida C. et al. 2011; Rani SA et al.
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2007), we’ve developed three different biofilm formation methods:
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Bubbling method, Rubber tube method and cell culture plate method. The
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biofilm formed in the two former ways were observed through
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cryo-sectioning coupled with normal fluorescence microscopy while
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confocal laser scanning microscope was employed to obtain the biofilm
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images of the third method. In addition to the above, we’ve tested
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different material’s applicability in biofilm formation experiments and
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introduced autoinducer AI-2 as an accelerator molecule.</p>
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Latest revision as of 17:34, 19 October 2011

  1. REDIRECT Team:ZJU-China/Project
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