Team:Tec-Monterrey/projectnotebook july/day12

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Latest revision as of 01:21, 18 October 2011

wiki

iGEM

 

 


12/July/2011
• Minipreps -> 14L col.3, 8G col.3 and 13L8G col 19
• Digestion with EcoRI , SpeI and XbaI ; 2m with PstI and SpeI
• Electrophoresis
• Chema gave us certain strains for the expression of the protein, which are: Roset Gami, XL1 Blue, C43, Pet 12, BL21, C41, Pet 28
• Purification -> 13L col.3 and 8G col.3
• Ligation -> 13L+8G
• Touchdown PCR for SacC, Program: 61-43ºC, Δ1ºC every 2 cycles.
• PCR product purification, quantification and overnight ligation in pGEM.


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