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<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> Notebook - September

Lab Notes

July August
September

Protocol

Brainstorm

Lab Notes - September

Biobrick Group

Week9

Day

Note

Aug.29th Monday

▪Meeting. Summarize all the experiment work so far.

▪Cut: 22M(X+P), 1C3(E+P)

Aug.30th Tuesday

Aug.31st Wednesday

▪Cut vgb+YFP+tetR, fdhF+RFP+tetR.
▪Run the digestion results

▪Culture the positive results

Sept.1st Thursday

▪Miniprep: Y#4, R#2/3/4/5
▪Cut Y#4, R#2/3/4/5

▪un the gel.
▪Send R#3,Y#4 sample to sequencing

▪Hypoxia culture: RFP3, RFP4
▪Preserve Y#4, R#2/3/4/5

Sept.2nd Friday

▪Phusion PCR

▪Hypoxia Culture: R, Y
▪Run the PCR results. Bands are confirmed right.

▪Purification: vgb, YFP, tetR.

Sept.3rd Saturday

▪Culture: YFP, RFP, A25
▪Culture in micro-oxygen: YFP, RFP, A25

▪Culture in slope medium: RFP, YFP, A25
▪Hypoxia culture: RFP, YFP, A25

▪Check the fluorescence.
▪Neither RFP in hypoxia or oxygen condition. Little YFP both in hypoxia and oxygen.

Sept.4th Sunday

▪Miniprep: RFP3, YFP4

▪Cut: RFP3, YFP4, !C3.

Week10

Day

Note

Sept.5th Monday

▪Run the gel: Yu's PCR results, 1C3, RFP3, YFP4
▪Purification: RFP3, YFP4

▪Ligation: RFP3+1C3, YFP4+1C3
▪Hypoxia culture: RFP3, YFP4, A25.

▪Transform the ligation results.
▪Confirmed RFP expression only in hypoxia condition.

Sept.6th Tuesday

▪Check the RFP, YFP plates.

▪Colony PCR

Sept.7th Wednesday

▪Culture YFP1/2, RFP3/4

Sept.8th Thurday

▪Preserve the strain of RFP1, YFP1/2 in 1C3.
▪Miniprep the remains.

▪Cut: YFP1/2, RFP1

▪Run the digestion results.
▪Culture RFP2

Sept.9th Friday

▪Miniprep: RFP2, YFP2, RFP1

▪Cut them and run the gel.

Sept.10th Saturday

▪Cut YFP(1A3), RFP(1A3) with S+E

▪Purification: RFP(1A3), YFP(1A3)

▪Ligation: RFP+1C3, YFP+1C3

▪Transform the ligation results.

Sept.11th Sunday

▪Check the plates.

▪Culture lig RFY+YFP+IC3
▪Culture 1K3

▪Colony PCR: RFP(1C3), YFP(1C3)

Week11

Day

Note

Sept.12th Monday

▪Colony PCR: 1A3, 1K3, lig RFP+YFP+1C3.

▪Run the PCR results.

▪Miniprep: lig RFP+YFP+1C3

Sept.13th Tuesday

▪Transform 2M, 2I, 6I, 23L.

▪Purification: 1K3

Sept.14th Wednesday

▪Miniprep: 20H, 20J, 22B
▪Cut: 1K3 with E+P, lig3 with S+E,
12I(CFP) with P+X,

▪Gel excision and /or purification:
1K3, lig3, 12I(CFP)
▪Culture:
1I, 1K, 3L, 5E, 7C
▪Run the gel.
▪Miniprep:
RFP+YFP+1C3.

▪Run the gel of
1K3, lig3, CFP again.
▪Gel excision and purification
▪Ligation:
Lig3+CFP+1K3

Sept.15th Thursday

▪Preserve: 1I, 1K, 3C, 5E, 7C
▪Transform the ligation results

▪Colony PCR: 2M, 2I,6I,23L
▪Cut: 20H, 20J, 22B with X+P

▪Miniprep: 2M-1, 2I-1, 6I-1, 23L-1
▪Cut: 2M, 2I with E+S

Sept.16th Friday

▪Colony PCR: RFP+YFP+1K3

▪Cut 13K with E+S,
10I with P+X, 1C3 with E+P
▪Run the gel:
13K, 10I, 1C3, 2I, 2M, 20J, 22H, 22B

▪Ligation: 13K+10I+1C3
▪Culture: YFP(Amp), CFP(Amp)

Sept.17th Saturday

▪Order primers for 20H, 20J
▪Culture 20H-1, 20J-1, 22B-1

▪Plate the ligation results
▪Culture RFP+YFP+CFP+1K3

▪Colony PCR: 3A, 1C3
▪Preserve: 1K3, RFP+YFP+1C3.

Sept.18th Sunday

▪Miniprep: 20J, 20H, RFP+YFP+CFP+1K3 1A3]
▪Run the gel of PCR results

▪Cut RFP+YFP+CFP+1K3 with E+P
▪Colony PCR: 13K+10I+1C3
▪PCR: fdhF+rush+Vgb+tetR

▪Preserve: 20H, 20J, 22B
▪Run the gel: 20H, 20J, 22B
▪Cut the bands and purification.

▪Run the gel: 13K+10I+1C3, RFP+YFP+CFP+1K3
▪Culture: 13K+10I+1C3
▪Grad PCR: fdhF.

Week12

Day

Note

Sept.19th Monday

▪Miniprep: 13K+10I+1C3, 1C3
▪Run the gel of digestion results.

▪Cut: 13K+10I+1C3, 1A3, fdhF

▪Run the gel: 20H, 20J, rush+vgb, RYC+1K3, fdhF, 13K+10I, 1A3

▪Cut and purification: fdhF, 13K+10I, 1A3
▪Culture: CFP, YFP

▪Purification: Rush PCR
▪Cut: 22B with X, rush PCR results with S

Sept.20th Tuesday

▪Run the 20H, 20J from PCR results.
▪Transform: fdhF+13K+10I+1A3

▪Purification: 20B, Rush
▪Ligation: 22B+Rush

▪Purification: ligation results
▪Cut: the ligation results with X+S

▪PCR 20H
▪Self-ligation: rush+22M

Sept.21st Wednesday

▪Colony PCR: fdhF+13K+10I+1A3 on plates

▪Run the gel of PCR results

▪Culture the positive results.

Sept.22nd Thursday

▪Miniprep: fdhF+13K+10I+1A3
▪Preserve the above.

▪Cut and run the results: fdhF+13K+10I+1A3, 13K+10I+1C3

▪Cut: RFP with E+S, YFP with X+P, 1K3 with E+P, 1C3 with E+P

▪Purification the digestion results.

Sept.23rd Friday

▪Ligate: RFP+1A3/ YFP+1C3/1K3, RFP+1A3/1C3

▪Transform the ligation results.

Sept. 24th Saturday

▪Order primers for 20H, 20J
▪Culture 20H-1, 20J-1, 22B-1

▪Plate the ligation results
▪Culture RFP+YFP+CFP+1K3

▪Colony PCR: 3A, 1C3
▪Preserve: 1K3, RFP+YFP+1C3.

Sept.25th Sunday

▪Miniprep: 20J, 20H, RFP+YFP+CFP+1K3 1A3
▪Run the gel of PCR results

▪Cut RFP+YFP+CFP+1K3 with E+P
▪PCR: fdhF+rush+Vgb+tetR
▪Colony PCR: 13K+10I+1C3

▪Preserve: 20H, 20J, 22B
▪Run the gel: 20H, 20J, 22B
▪Cut the bands and purification.

▪Run the gel: 13K+10I+1C3, RFP+YFP+CFP+1K3
▪Culture: 13K+10I+1C3
▪Grad PCR: fdhF.

Biofilm Group

3rd Sep

Directly use DH5α fdhF+RFP colony to inoculate silicone tube set with 5ml LB. rest for 12h for biofilm attachment.

4th Sep

Add 50mlLB+Amp and start circulation.

6th Sep

Biofilm with direct inoculation is optimum. Expressed red florescence but only one small fraction of the biofilm remained after cutting off the part of the tube.

12th Sep

4 silicone tube sets with biofilm attachement time of 3h, 5h, 7.5h and 10h; 4 bubbling sets.

15th Sep

The silicone tube set with 3h of attachment achieves optimum biofilm formation. Bubbling sets glass slides are froze in -80鈩for later observation with laser confocal. Biofilm formed on glass slide is about 10μm thick.

16th Sep

New sets with silicone tube, a mixture of RFP, CFP and YFP expressing e.coli. Attachment 3h.

17th Sep

Glass slide with bubbling set with the same mixture of e.coli.
Place two filter film on solid culture medium and placed the mixture of e.coli on the filter to form gas-solid interface biofilm.

20th Sep

Observation of glass slide and gas-solid biofilm under laser confocal. Glass slide are observed directly and gas-solid biofilm is first pressed by a covering slide so that it would stick to it and peeled off from the filter for observation.
Biofilm on glass slide is about 8.6μm thick. Biofilm formed on gas-solid interface is too concentrated in bacteria and culture medium that the florescence of too strong to see any clear structure.

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@@ Line 192: / Line 192: @@
 			cellpadding="1">
-			<td width="260">鈥?Meeting. Summarize all the experiment work so
+			<td width="260">▪Meeting. Summarize all the experiment work so
 			far.</td>
-			<td width="297">鈥?Cut: 22M(X+P), 1C3(E+P)</td>
+			<td width="297">▪Cut: 22M(X+P), 1C3(E+P)</td>
@@ Line 219: / Line 219: @@
 		<table width="563" border="0" cellspacing="0" cellpadding="1">
 			<tr>
-				<td width="361">鈥?Cut vgb+YFP+tetR, fdhF+RFP+tetR.<br />
+				<td width="361">▪Cut vgb+YFP+tetR, fdhF+RFP+tetR.<br />
-				鈥?Run the digestion results</td>
+				▪Run the digestion results</td>
-				<td width="198">鈥?Culture the positive results</td>
+				<td width="198">▪Culture the positive results</td>
 			</tr>
 		</table>
@@ Line 232: / Line 232: @@
 			<tr>
-				<td width="171">鈥?Miniprep: Y#4, R#2/3/4/5<br />
+				<td width="171">▪Miniprep: Y#4, R#2/3/4/5<br />
-				鈥?Cut Y#4, R#2/3/4/5</td>
+				▪Cut Y#4, R#2/3/4/5</td>
-				<td width="250">鈥un the gel.<br />
+				<td width="250">▪un the gel.<br />
-				鈥?Send R#3,Y#4 sample to sequencing</td>
+				▪Send R#3,Y#4 sample to sequencing</td>
-				<td width="145">鈥?Hypoxia culture: RFP3, RFP4<br />
+				<td width="145">▪Hypoxia culture: RFP3, RFP4<br />
-				鈥?Preserve Y#4, R#2/3/4/5</td>
+				▪Preserve Y#4, R#2/3/4/5</td>
 			</tr>
 		</table>
@@ Line 247: / Line 247: @@
 		<table width="572" border="0" cellspacing="0" cellpadding="1">
 			<tr>
-				<td width="92">鈥?Phusion PCR</td>
+				<td width="92">▪Phusion PCR</td>
-				<td width="276">鈥?Hypoxia Culture: R, Y<br />
+				<td width="276">▪Hypoxia Culture: R, Y<br />
-				鈥?Run the PCR results. Bands are confirmed right.</td>
+				▪Run the PCR results. Bands are confirmed right.</td>
-				<td width="198">鈥?Purification: vgb, YFP, tetR.</td>
+				<td width="198">▪Purification: vgb, YFP, tetR.</td>
 			</tr>
 		</table>
@@ Line 260: / Line 260: @@
 		<table width="569" border="0" cellspacing="0" cellpadding="1">
 			<tr>
-				<td width="152">鈥?Culture: YFP, RFP, A25<br />
+				<td width="152">▪Culture: YFP, RFP, A25<br />
-				鈥?Culture in micro-oxygen: YFP, RFP, A25</td>
+				▪Culture in micro-oxygen: YFP, RFP, A25</td>
-				<td width="149">鈥?Culture in slope medium: RFP, YFP, A25<br />
+				<td width="149">▪Culture in slope medium: RFP, YFP, A25<br />
-				鈥?Hypoxia culture: RFP, YFP, A25</td>
+				▪Hypoxia culture: RFP, YFP, A25</td>
-				<td width="262">鈥?Check the fluorescence.<br />
+				<td width="262">▪Check the fluorescence.<br />
-				鈥?Neither RFP in hypoxia or oxygen condition. Little YFP both in
+				▪Neither RFP in hypoxia or oxygen condition. Little YFP both in
 				hypoxia and oxygen.</td>
 			</tr>
@@ Line 277: / Line 277: @@
 			cellspacing="0">
 			<tr>
-				<td width="285">鈥?Miniprep: RFP3, YFP4</td>
+				<td width="285">▪Miniprep: RFP3, YFP4</td>
-				<td width="331">鈥?Cut: RFP3, YFP4, !C3.</td>
+				<td width="331">▪Cut: RFP3, YFP4, !C3.</td>
 			</tr>
@@ Line 301: / Line 301: @@
 			cellpadding="1">
-			<td width="191">鈥?Run the gel: Yu's PCR results, 1C3, RFP3, YFP4<br />
+			<td width="191">▪Run the gel: Yu's PCR results, 1C3, RFP3, YFP4<br />
-			鈥?Purification: RFP3, YFP4</td>
+			▪Purification: RFP3, YFP4</td>
-			<td>鈥?Ligation: RFP3+1C3, YFP4+1C3<br />
+			<td>▪Ligation: RFP3+1C3, YFP4+1C3<br />
-			鈥?Hypoxia culture: RFP3, YFP4, A25.</td>
+			▪Hypoxia culture: RFP3, YFP4, A25.</td>
-			<td>鈥?Transform the ligation results.<br />
+			<td>▪Transform the ligation results.<br />
-			鈥?Confirmed RFP expression only in hypoxia condition.</td>
+			▪Confirmed RFP expression only in hypoxia condition.</td>
 		</table>
@@ Line 318: / Line 318: @@
 		<table id="intable" width="600" border="0" cellspacing="0"
 			cellpadding="1">
-			<td width="414">鈥?Check the RFP, YFP plates.</td>
+			<td width="414">▪Check the RFP, YFP plates.</td>
-			<td width="182">鈥?Colony PCR</td>
+			<td width="182">▪Colony PCR</td>
 		</table>
@@ Line 329: / Line 329: @@
 		<table width="609" border="0" cellspacing="0" cellpadding="1">
 			<tr>
-				<td width="607">鈥?Culture YFP1/2, RFP3/4</td>
+				<td width="607">▪Culture YFP1/2, RFP3/4</td>
 			</tr>
@@ Line 341: / Line 341: @@
 			<tr>
-				<td width="297">鈥?Preserve the strain of RFP1, YFP1/2 in 1C3.<br />
+				<td width="297">▪Preserve the strain of RFP1, YFP1/2 in 1C3.<br />
-				鈥?Miniprep the remains.</td>
+				▪Miniprep the remains.</td>
-				<td width="140">鈥?Cut: YFP1/2, RFP1</td>
+				<td width="140">▪Cut: YFP1/2, RFP1</td>
-				<td width="154">鈥?Run the digestion results.<br />
+				<td width="154">▪Run the digestion results.<br />
-				鈥?Culture RFP2</td>
+				▪Culture RFP2</td>
 			</tr>
 		</table>
@@ Line 355: / Line 355: @@
 		<table width="612" border="0" cellspacing="0" cellpadding="1">
 			<tr>
-				<td width="335">鈥?Miniprep: RFP2, YFP2, RFP1</td>
+				<td width="335">▪Miniprep: RFP2, YFP2, RFP1</td>
-				<td width="273">鈥?Cut them and run the gel.</td>
+				<td width="273">▪Cut them and run the gel.</td>
 			</tr>
@@ Line 367: / Line 367: @@
 		<table width="610" border="0" cellspacing="0" cellpadding="1">
 			<tr>
-				<td width="161">鈥?Cut YFP(1A3), RFP(1A3) with S+E</td>
+				<td width="161">▪Cut YFP(1A3), RFP(1A3) with S+E</td>
-				<td width="162">鈥?Purification: RFP(1A3), YFP(1A3)</td>
+				<td width="162">▪Purification: RFP(1A3), YFP(1A3)</td>
-				<td width="148">鈥?Ligation: RFP+1C3, YFP+1C3</td>
+				<td width="148">▪Ligation: RFP+1C3, YFP+1C3</td>
-				<td width="131">鈥?Transform the ligation results.</td>
+				<td width="131">▪Transform the ligation results.</td>
 			</tr>
 		</table>
@@ Line 380: / Line 380: @@
 		<table width="608" border="0" cellspacing="0" cellpadding="1">
 			<tr>
-				<td width="244">鈥?Check the plates.</td>
+				<td width="244">▪Check the plates.</td>
-				<td width="164">鈥?Culture lig RFY+YFP+IC3<br />
+				<td width="164">▪Culture lig RFY+YFP+IC3<br />
-				鈥?Culture 1K3</td>
+				▪Culture 1K3</td>
-				<td width="194">鈥?Colony PCR: RFP(1C3)锛?YFP(1C3)</td>
+				<td width="194">▪Colony PCR: RFP(1C3), YFP(1C3)</td>
 			</tr>
 		</table>
@@ Line 404: / Line 404: @@
 			cellpadding="1">
 			<tr>
-				<td width="191">鈥?Colony PCR: 1A3, 1K3, lig RFP+YFP+1C3.</td>
+				<td width="191">▪Colony PCR: 1A3, 1K3, lig RFP+YFP+1C3.</td>
-				<td>鈥?Run the PCR results.</td>
+				<td>▪Run the PCR results.</td>
-				<td>鈥?Miniprep: lig RFP+YFP+1C3</td>
+				<td>▪Miniprep: lig RFP+YFP+1C3</td>
 			</tr>
 		</table>
@@ Line 416: / Line 416: @@
 		<table id="intable3" border="0" cellspacing="0" cellpadding="1">
 			<tr>
-				<td>鈥?Transform 2M, 2I, 6I, 23L.</td>
+				<td>▪Transform 2M, 2I, 6I, 23L.</td>
-				<td>鈥?Purification: 1K3</td>
+				<td>▪Purification: 1K3</td>
 			</tr>
 		</table>
@@ Line 427: / Line 427: @@
 		<table width="600" border="0" cellspacing="0" cellpadding="1">
 			<tr>
-				<td>鈥?Miniprep: 20H, 20J, 22B<br />
+				<td>▪Miniprep: 20H, 20J, 22B<br />
-				鈥?Cut: 1K3 with E+P, lig3 with S+E,<br />
+				▪Cut: 1K3 with E+P, lig3 with S+E,<br />
 I(CFP) with P+X,</td>
-				<td>鈥?Gel excision and /or purification:<br />
+				<td>▪Gel excision and /or purification:<br />
 K3, lig3, 12I(CFP)<br />
-				鈥?Culture: <br />
+				▪Culture: <br />
 I, 1K, 3L, 5E, 7C<br />
-				鈥?Run the gel.<br />
+				▪Run the gel.<br />
-				鈥?Miniprep: <br />
+				▪Miniprep: <br />
 				RFP+YFP+1C3.<br />
 				</td>
-				<td>鈥?Run the gel of<br />
+				<td>▪Run the gel of<br />
 K3, lig3, CFP again.<br />
-				鈥?Gel excision and purification<br />
+				▪Gel excision and purification<br />
-				鈥?Ligation:<br />
+				▪Ligation:<br />
 				Lig3+CFP+1K3</td>
 			</tr>
@@ Line 452: / Line 452: @@
 		<table width="618" border="0" cellspacing="0" cellpadding="1">
 			<tr>
-				<td>鈥?Preserve: 1I, 1K, 3C, 5E, 7C<br />
+				<td>▪Preserve: 1I, 1K, 3C, 5E, 7C<br />
-				鈥?Transform the ligation results</td>
+				▪Transform the ligation results</td>
-				<td>鈥?Colony PCR: 2M, 2I锛?I锛?3L<br />
+				<td>▪Colony PCR: 2M, 2I,6I,23L<br />
-				鈥?Cut: 20H, 20J, 22B with X+P</td>
+				▪Cut: 20H, 20J, 22B with X+P</td>
-				<td>鈥?Miniprep: 2M-1, 2I-1, 6I-1, 23L-1<br />
+				<td>▪Miniprep: 2M-1, 2I-1, 6I-1, 23L-1<br />
-				鈥?Cut: 2M, 2I with E+S</td>
+				▪Cut: 2M, 2I with E+S</td>
 			</tr>
 		</table>
@@ Line 467: / Line 467: @@
 		<table width="600" border="0" cellspacing="0" cellpadding="1">
 			<tr>
-				<td>鈥?Colony PCR: RFP+YFP+1K3</td>
+				<td>▪Colony PCR: RFP+YFP+1K3</td>
-				<td>鈥?Cut 13K with E+S, <br />
+				<td>▪Cut 13K with E+S, <br />
 I with P+X, 1C3 with E+P<br />
-				鈥?Run the gel: <br />
+				▪Run the gel: <br />
 K, 10I, 1C3, 2I, 2M, 20J, 22H, 22B</td>
-				<td>鈥?Ligation: 13K+10I+1C3<br />
+				<td>▪Ligation: 13K+10I+1C3<br />
-				鈥?Culture: YFP(Amp), CFP(Amp)</td>
+				▪Culture: YFP(Amp), CFP(Amp)</td>
 			</tr>
 		</table>
@@ Line 483: / Line 483: @@
 		<table width="620" border="0" cellspacing="0" cellpadding="1">
 			<tr>
-				<td width="169">鈥?Order primers for 20H, 20J<br />
+				<td width="169">▪Order primers for 20H, 20J<br />
-				鈥?Culture 20H-1, 20J-1, 22B-1</td>
+				▪Culture 20H-1, 20J-1, 22B-1</td>
-				<td width="225">鈥?Plate the ligation results<br />
+				<td width="225">▪Plate the ligation results<br />
-				鈥?Culture RFP+YFP+CFP+1K3</td>
+				▪Culture RFP+YFP+CFP+1K3</td>
-				<td width="220">鈥?Colony PCR: 3A, 1C3<br />
+				<td width="220">▪Colony PCR: 3A, 1C3<br />
-				鈥?Preserve: 1K3, RFP+YFP+1C3.</td>
+				▪Preserve: 1K3, RFP+YFP+1C3.</td>
 			</tr>
 		</table>
@@ Line 498: / Line 498: @@
 		<table width="620" border="0" cellspacing="0" cellpadding="1">
 			<tr>
-				<td width="120">鈥?Miniprep: 20J, 20H, RFP+YFP+CFP+1K3 1A3]<br />
+				<td width="120">▪Miniprep: 20J, 20H, RFP+YFP+CFP+1K3 1A3]<br />
-				鈥?Run the gel of PCR results</td>
+				▪Run the gel of PCR results</td>
-				<td>鈥?Cut RFP+YFP+CFP+1K3 with E+P<br />
+				<td>▪Cut RFP+YFP+CFP+1K3 with E+P<br />
-				鈥?Colony PCR: 13K+10I+1C3<br />
+				▪Colony PCR: 13K+10I+1C3<br />
-				鈥?PCR: fdhF+rush+Vgb+tetR</td>
+				▪PCR: fdhF+rush+Vgb+tetR</td>
 				<td></td>
-				<td>鈥?Preserve: 20H, 20J, 22B<br />
+				<td>▪Preserve: 20H, 20J, 22B<br />
-				鈥?Run the gel: 20H, 20J, 22B<br />
+				▪Run the gel: 20H, 20J, 22B<br />
-				鈥?Cut the bands and purification.</td>
+				▪Cut the bands and purification.</td>
-				<td>鈥?Run the gel: 13K+10I+1C3, RFP+YFP+CFP+1K3<br />
+				<td>▪Run the gel: 13K+10I+1C3, RFP+YFP+CFP+1K3<br />
-				鈥?Culture: 13K+10I+1C3<br />
+				▪Culture: 13K+10I+1C3<br />
-				鈥?Grad PCR: fdhF.</td>
+				▪Grad PCR: fdhF.</td>
 			</tr>
 		</table>
@@ Line 528: / Line 528: @@
 			cellpadding="1">
 			<tr>
-				<td width="191">鈥?Miniprep: 13K+10I+1C3, 1C3<br />
+				<td width="191">▪Miniprep: 13K+10I+1C3, 1C3<br />
-				鈥?Run the gel of digestion results.</td>
+				▪Run the gel of digestion results.</td>
-				<td width="97">鈥?Cut: 13K+10I+1C3, 1A3, fdhF</td>
+				<td width="97">▪Cut: 13K+10I+1C3, 1A3, fdhF</td>
-				<td width="112">鈥?Run the gel: 20H, 20J, rush+vgb, RYC+1K3,
+				<td width="112">▪Run the gel: 20H, 20J, rush+vgb, RYC+1K3,
 				fdhF, 13K+10I, 1A3</td>
-				<td width="94">鈥?Cut and purification: fdhF, 13K+10I, 1A3<br />
+				<td width="94">▪Cut and purification: fdhF, 13K+10I, 1A3<br />
-				鈥?Culture: CFP, YFP</td>
+				▪Culture: CFP, YFP</td>
-				<td width="88">鈥?Purification: Rush PCR<br />
+				<td width="88">▪Purification: Rush PCR<br />
-				鈥?Cut: 22B with X, rush PCR results with S</td>
+				▪Cut: 22B with X, rush PCR results with S</td>
 			</tr>
 		</table>
@@ Line 547: / Line 547: @@
 			cellpadding="1">
 			<tr>
-				<td width="189">鈥?Run the 20H, 20J from PCR results.<br />
+				<td width="189">▪Run the 20H, 20J from PCR results.<br />
-				鈥?Transform: fdhF+13K+10I+1A3</td>
+				▪Transform: fdhF+13K+10I+1A3</td>
-				<td width="122">鈥?Purification: 20B, Rush<br />
+				<td width="122">▪Purification: 20B, Rush<br />
-				鈥?Ligation: 22B+Rush</td>
+				▪Ligation: 22B+Rush</td>
-				<td width="169">鈥?Purification: ligation results<br />
+				<td width="169">▪Purification: ligation results<br />
-				鈥?Cut: the ligation results with X+S</td>
+				▪Cut: the ligation results with X+S</td>
-				<td width="113">鈥?PCR 20H<br />
+				<td width="113">▪PCR 20H<br />
-				鈥?Self-ligation: rush+22M</td>
+				▪Self-ligation: rush+22M</td>
 			</tr>
 		</table>
@@ Line 564: / Line 564: @@
 		<table width="603" border="0" cellspacing="0" cellpadding="1">
 			<tr>
-				<td width="276">鈥?Colony PCR: fdhF+13K+10I+1A3 on plates</td>
+				<td width="276">▪Colony PCR: fdhF+13K+10I+1A3 on plates</td>
-				<td width="177">鈥?Run the gel of PCR results</td>
+				<td width="177">▪Run the gel of PCR results</td>
-				<td width="144">鈥?Culture the positive results.</td>
+				<td width="144">▪Culture the positive results.</td>
 			</tr>
 		</table>
@@ Line 576: / Line 576: @@
 		<table width="611" border="0" cellspacing="0" cellpadding="1">
 			<tr>
-				<td width="130">鈥?Miniprep: fdhF+13K+10I+1A3<br />
+				<td width="130">▪Miniprep: fdhF+13K+10I+1A3<br />
-				鈥?Preserve the above.</td>
+				▪Preserve the above.</td>
-				<td width="202">鈥?Cut and run the results: fdhF+13K+10I+1A3,
+				<td width="202">▪Cut and run the results: fdhF+13K+10I+1A3,
 K+10I+1C3</td>
-				<td width="162">鈥?Cut: RFP with E+S, YFP with X+P, 1K3 with
+				<td width="162">▪Cut: RFP with E+S, YFP with X+P, 1K3 with
 				E+P, 1C3 with E+P</td>
-				<td width="109">鈥?Purification the digestion results.</td>
+				<td width="109">▪Purification the digestion results.</td>
 			</tr>
 		</table>
@@ Line 592: / Line 592: @@
 		<table width="600" border="0" cellspacing="0" cellpadding="1">
 			<tr>
-				<td width="376">鈥?Ligate: RFP+1A3/ YFP+1C3/1K3, RFP+1A3/1C3</td>
+				<td width="376">▪Ligate: RFP+1A3/ YFP+1C3/1K3, RFP+1A3/1C3</td>
-				<td width="220">鈥?Transform the ligation results.</td>
+				<td width="220">▪Transform the ligation results.</td>
 			</tr>
 		</table>
@@ Line 603: / Line 603: @@
 		<table width="604" border="0" cellspacing="0" cellpadding="1">
 			<tr>
-				<td width="197">鈥?Order primers for 20H, 20J<br />
+				<td width="197">▪Order primers for 20H, 20J<br />
-				鈥?Culture 20H-1, 20J-1, 22B-1</td>
+				▪Culture 20H-1, 20J-1, 22B-1</td>
-				<td width="200">鈥?Plate the ligation results<br />
+				<td width="200">▪Plate the ligation results<br />
-				鈥?Culture RFP+YFP+CFP+1K3</td>
+				▪Culture RFP+YFP+CFP+1K3</td>
-				<td width="201">鈥?Colony PCR: 3A, 1C3<br />
+				<td width="201">▪Colony PCR: 3A, 1C3<br />
-				鈥?Preserve: 1K3, RFP+YFP+1C3.</td>
+				▪Preserve: 1K3, RFP+YFP+1C3.</td>
 			</tr>
 		</table>
@@ Line 618: / Line 618: @@
 		<table width="613" border="0" cellspacing="0" cellpadding="1">
 			<tr>
-				<td width="121">鈥?Miniprep: 20J, 20H, RFP+YFP+CFP+1K3 1A3<br />
+				<td width="121">▪Miniprep: 20J, 20H, RFP+YFP+CFP+1K3 1A3<br />
-				鈥?Run the gel of PCR results</td>
+				▪Run the gel of PCR results</td>
-				<td width="116">鈥?Cut RFP+YFP+CFP+1K3 with E+P<br />
+				<td width="116">▪Cut RFP+YFP+CFP+1K3 with E+P<br />
-				鈥?PCR: fdhF+rush+Vgb+tetR<br />
+				▪PCR: fdhF+rush+Vgb+tetR<br />
-				鈥?Colony PCR: 13K+10I+1C3</td>
+				▪Colony PCR: 13K+10I+1C3</td>
-				<td width="66">鈥?Preserve: 20H, 20J, 22B<br />
+				<td width="66">▪Preserve: 20H, 20J, 22B<br />
-				鈥?Run the gel: 20H, 20J, 22B<br />
+				▪Run the gel: 20H, 20J, 22B<br />
-				鈥?Cut the bands and purification.</td>
+				▪Cut the bands and purification.</td>
-				<td width="112">鈥?Run the gel: 13K+10I+1C3, RFP+YFP+CFP+1K3<br />
+				<td width="112">▪Run the gel: 13K+10I+1C3, RFP+YFP+CFP+1K3<br />
-				鈥?Culture: 13K+10I+1C3<br />
+				▪Culture: 13K+10I+1C3<br />
-				鈥?Grad PCR: fdhF.</td>
+				▪Grad PCR: fdhF.</td>
 			</tr>
 		</table>
@@ Line 651: / Line 651: @@
-<p>Directly use DH5伪 fdhF+RFP colony to inoculate silicone tube set
+<p>Directly use DH5α fdhF+RFP colony to inoculate silicone tube set
 with 5ml LB. rest for 12h for biofilm attachment.</p>
 </div>
@@ Line 672: / Line 672: @@
 <h1>15th Sep</h1>
 <p>The silicone tube set with 3h of attachment achieves optimum
-biofilm formation. Bubbling sets glass slides are froze in -80鈩?for
+biofilm formation. Bubbling sets glass slides are froze in -80鈩for
 later observation with laser confocal. Biofilm formed on glass slide is
-about 10渭m thick.</p>
+about 10μm thick.</p>
 </div>
 <div class="block" id="nsheet">
@@ Line 693: / Line 693: @@
 first pressed by a covering slide so that it would stick to it and
 peeled off from the filter for observation.<br />
-Biofilm on glass slide is about 8.6渭m thick. Biofilm formed on gas-solid
+Biofilm on glass slide is about 8.6μm thick. Biofilm formed on gas-solid
 interface is too concentrated in bacteria and culture medium that the
 florescence of too strong to see any clear structure.</p>
@@ Line 732: / Line 732: @@
 iGem 2011 Home Page</a> <a href="http://ung.igem.org/Special:Upload/">
 Upload Files</a> <a
-	href="https://2011.igem.org/wiki/index.php?title=Template:Zjucss_main&action=edit">Edit
+	href="https://2011.igem.org/wiki/index.phptitle=Template:Zjucss_main&action=edit">Edit
-CSS</a> <a href="http://ung.igem.org/Team_Wikis?year=2011"> Team Wikis</a> <a
+CSS</a> <a href="http://ung.igem.org/Team_Wikisyear=2011"> Team Wikis</a> <a
-	href="mailto:zjuigem@gmail.com?"><strong>Contact Us</strong></a></div>
+	href="mailto:zjuigem@gmail.com"><strong>Contact Us</strong></a></div>
 </div>
 <!--contentwrapper--></div>

Team:ZJU-China/September.html

From 2011.igem.org

Revision as of 09:38, 17 October 2011

Lab Notes

Protocol

Brainstorm

Lab Notes - September

Biobrick Group

Week9

Week10

Week11

Week12

Biofilm Group

3rd Sep

4th Sep

6th Sep

12th Sep

15th Sep

16th Sep

17th Sep

20th Sep