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Team:IIT Madras/Notebook
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<li>Literature survey on expression of Proteorhodopsin in E.coli</li></br></br> | <li>Literature survey on expression of Proteorhodopsin in E.coli</li></br></br> | ||
<h2>Week 2</h2> | <h2>Week 2</h2> | ||
| - | <li>Minimal media characterization of DH5-alpha | + | <li>Minimal media characterization of DH5-alpha |
| - | <li>Modelling survival of PR transformed E.coli cells v/s wild type E.coli | + | <li>Modelling survival of PR transformed E.coli cells v/s wild type E.coli |
| - | <li>Literature survey on increase in recombinant protein expression in PR transformed cells | + | <li>Literature survey on increase in recombinant protein expression in PR transformed cells</br></br> |
<h2>Week 3</h2> | <h2>Week 3</h2> | ||
| - | <li>Obtained Proteorhodopsin gene and Beta-Dioxygenase in PKJ-900 from Kwang-Hwan "Kevin" Jung, Ph.D., Associate Professor, Department of Life Science and Institute of Biological Interfaces, Sogang University, Korea. | + | <li>Obtained Proteorhodopsin gene and Beta-Dioxygenase in PKJ-900 from Kwang-Hwan "Kevin" Jung, Ph.D., Associate Professor, Department of Life Science and Institute of Biological Interfaces, Sogang University, Korea. |
| - | <li>Designing the incubator for experimental studies with Proteorhodopsin | + | <li>Designing the incubator for experimental studies with Proteorhodopsin |
| - | <li>Experimental analysis of growth rates of PR transformed E.coli and DH5-alpha in presence of ETC-Inhibitor Azide | + | <li>Experimental analysis of growth rates of PR transformed E.coli and DH5-alpha in presence of ETC-Inhibitor Azide |
<h2>Week 4</h2> | <h2>Week 4</h2> | ||
| - | <li>Standardizing the protocol for digestion of pKJ900 | + | <li>Standardizing the protocol for digestion of pKJ900 |
| - | <li>Designing RFP generator for promoter studies | + | <li>Designing RFP generator for promoter studies |
<h2>Week5</h2> | <h2>Week5</h2> | ||
| - | <li>Generating the PcstA_RFP Generator | + | <li>Generating the PcstA_RFP Generator |
| - | <li>Synthesizing the T7_RFP Generator | + | <li>Synthesizing the T7_RFP Generator |
<h2>Week6</h2> | <h2>Week6</h2> | ||
| - | <li>Characterizing PcstA_RFP Generator</li> | + | <li>Characterizing PcstA_RFP Generator |
| + | <li>Designing the promoters for Proteorhodopsin gene | ||
| + | <li>Codon optimization of Proteorhodopsin gene | ||
| + | <h2>Week7</h2> | ||
| + | <li>Designing the OmpR Promoter_RFP Generator | ||
| + | <li>Designing the PcstA_Luciferase Generator | ||
| + | <li>Digestion of Promoters | ||
| + | <h2>Week8</h2> | ||
| + | <li>Transformation of Promoters | ||
| + | <li>Standardizing the protocols for transformation of promoters | ||
| + | <li>Designing the Proteorhodopsin Generator | ||
Revision as of 03:21, 6 October 2011
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