Team:HokkaidoU Japan/Project/RFC87

From 2011.igem.org

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Revision as of 03:09, 6 October 2011

Contents

  • Abstract
  • What`s T3SS
    Detailed information about T3SS and summary of our achievements on iGEM 2010
  • Injection assay using onion cells
    Experiments using plant cells are easier to perform than with mammalian ones
  • Ready-to-inject backbone and Bsa I cloning site
    Ready-to-inject backbone and Bsa I cloning site enables easy fusion of T3S signal and protein
  • GSK tag system
    A neat injection assay using GSK tag, which can specifically detect successfully injected proteins
  • Bsa I cloning site, RFC submission
    Detailed documentation of costructing a BioBrick cloning site a BioBrick!

<img src="HokkaidoU_BsaI-1.png">

<img src="HokkaidoU_BsaI-2.png">

<img src="HokkaidoU_BsaI-3.png">

<img src="HokkaidoU_BsaI-4.png">

<img src="HokkaidoU_BsaI-5.png">

<img src="HokkaidoU_BsaI-6.png">



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