Team:IIT Madras/Notebook

From 2011.igem.org

(Difference between revisions)
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<li>Standardizing the protocol for digestion of pKJ900</li>
<li>Standardizing the protocol for digestion of pKJ900</li>
<li>Designing RFP generator for promoter studies</li>
<li>Designing RFP generator for promoter studies</li>
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<h2>Week5<h2>
+
<h2>Week5</h2>
<li>Generating the PcstA_RFP Generator</li>
<li>Generating the PcstA_RFP Generator</li>
<li>Synthesizing the T7_RFP Generator</li>
<li>Synthesizing the T7_RFP Generator</li>
 +
<h2>Week6</h2>
 +
<li>Characterizing PcstA_RFP Generator</li>

Revision as of 03:02, 6 October 2011

bar iGEM 2011 - Home Page Indian Institute of Technology - Madras




Contents

Notebook

Week 1

  • Metabolic simulation and modelling of increase in proton flux on addition of Proteorhodopsin</li>
  • Literature survey on expression of Proteorhodopsin in E.coli</li></br></br>

    Week 2

  • Minimal media characterization of DH5-alpha</li>
  • Modelling survival of PR transformed E.coli cells v/s wild type E.coli</li>
  • Literature survey on increase in recombinant protein expression in PR transformed cells</li></br></br>

    Week 3

  • Obtained Proteorhodopsin gene and Beta-Dioxygenase in PKJ-900 from Kwang-Hwan "Kevin" Jung, Ph.D., Associate Professor, Department of Life Science and Institute of Biological Interfaces, Sogang University, Korea.</li>
  • Designing the incubator for experimental studies with Proteorhodopsin</li>
  • Experimental analysis of growth rates of PR transformed E.coli and DH5-alpha in presence of ETC-Inhibitor Azide</li>

    Week 4

  • Standardizing the protocol for digestion of pKJ900</li>
  • Designing RFP generator for promoter studies</li>

    Week5

  • Generating the PcstA_RFP Generator</li>
  • Synthesizing the T7_RFP Generator</li>

    Week6

  • Characterizing PcstA_RFP Generator</li>
    • Retrieved from "http://2011.igem.org/Team:IIT_Madras/Notebook"