Team:IIT Madras/Dry lab/Tools

From 2011.igem.org

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<li> No 3' Breathing should be ensured by keeping G or C at the end</li>
<li> No 3' Breathing should be ensured by keeping G or C at the end</li>
<li> GC content:40-60%
<li> GC content:40-60%
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<b><u>Basic Topology Properties</b></u>
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<li>Species: 1668 </li>
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<li>Reactions: 2383</li>
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<li>Lethal Genes:</li>
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<li>Genes that have no effect on knock out</li>
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<p align="center"><img src="https://static.igem.org/mediawiki/2011/e/e1/Tool-1.jpg" width="800" height="600"/></p><br/><br/>
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<p align="center"><img src="https://static.igem.org/mediawiki/2011/d/d9/Tool-2.jpg" width="800" height="600"/></p>
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Revision as of 02:55, 6 October 2011

bar iGEM 2011 - Home Page Indian Institute of Technology - Madras








Site directed Mutagenesis-Primer Design

We received proteorhodopsin sequence from Kwang-Hwan "Kevin" Jung, Ph.D., Associate Professor, Department of Life Science and Institute of Biological Interfaces, Sogang University with three restriction sites EcoRI, SpeI and PstI. Hence the plasmid had to be mutated for iGEM compatibility. Hence we had to design primers for site directed mutagenesis at each of these sites. So we have come up with a Primer design tool for SDM as a Criteria:

  1. Mutations in the middle of primer
  2. Tm difference should be less than 5
  3. No 3' Breathing should be ensured by keeping G or C at the end
  4. GC content:40-60%

Basic Topology Properties
  1. Species: 1668
  2. Reactions: 2383
  3. Lethal Genes:
  4. Genes that have no effect on knock out




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