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| | <table width="600" border="0" cellspacing="0" cellpadding="0"> | | <table width="600" border="0" cellspacing="0" cellpadding="0"> |
| | <tr> | | <tr> |
| - | <th height="38" colspan="5" bgcolor="#DCF8A9" class="notbookmaintitle" scope="col">Xiaowei Yan's Notebook</th> | + | <th height="38" colspan="5" bgcolor="#FFFFFF" class="notbookmaintitle" scope="col">Yan Xiaowei's Notebook</th> |
| | </tr> | | </tr> |
| | <tr> | | <tr> |
| - | <th colspan="5" bgcolor="#DCF8A9" class="notbookmaintitle" scope="col"><hr /></th> | + | <th colspan="5" bgcolor="#FFFFFF" class="notbookmaintitle" scope="col"><hr /></th> |
| | </tr> | | </tr> |
| | <tr> | | <tr> |
| - | <th width="16" height="94" bgcolor="# DCF8A9" scope="row"><p> </p> | + | <th width="16" height="94" bgcolor="# FFFFFF" scope="row"><p> </p> |
| | <p> </p></th> | | <p> </p></th> |
| - | <th colspan="2" bgcolor="# DCF8A9" scope="row"><p class="selfstatement3">I am in charge of the construction of c-di-GMP and theophylline ribozymes, focusing on the response properties of these ribozymes to corresponding ligands ---- c-di-GMP & theophylline. I am also the designer of our team's shirt.</p></th> | + | <th colspan="2" bgcolor="# FFFFFF" scope="row"><p class="selfstatement3">I am in charge of the construction of c-di-GMP and theophylline ribozymes, focusing on the response properties of these ribozymes to corresponding ligands ---- c-di-GMP & theophylline. I am also the designer of our team's shirt.</p></th> |
| - | <th width="125" rowspan="6" bgcolor="# DCF8A9" scope="row"><p><a href="https://2011.igem.org/Team:Peking_R/Team/YXW"><img src="https://static.igem.org/mediawiki/2011/c/cd/PekingR_xiaowei11.jpg" alt="" width="100" height="140" /></a><a href="https://2011.igem.org/Team:Peking_R/Team/ZMQ"></a><a href="https://2011.igem.org/Team:Peking_R/Team/ZZR"></a></p></th> | + | <th width="125" rowspan="6" bgcolor="# FFFFFF" scope="row"><p><a href="https://2011.igem.org/Team:Peking_R/Team/YXW"><img src="https://static.igem.org/mediawiki/2011/c/cd/PekingR_xiaowei11.jpg" alt="" width="100" height="140" /></a><a href="https://2011.igem.org/Team:Peking_R/Team/ZMQ"></a><a href="https://2011.igem.org/Team:Peking_R/Team/ZZR"></a></p></th> |
| - | <th width="20" bgcolor="#DCF8A9" scope="row"> </th> | + | <th width="20" bgcolor="#FFFFFF" scope="row"> </th> |
| | </tr> | | </tr> |
| | <tr> | | <tr> |
| - | <th rowspan="40" bgcolor="#DCF8A9" scope="row"> </th> | + | <th rowspan="14" bgcolor="#FFFFFF" scope="row"> </th> |
| - | <th width="29" height="29" bgcolor="# DCF8A9" scope="row"><img src="https://static.igem.org/mediawiki/2011/0/0c/PekingR_PDF_download_logo.png" alt="" width="30" height="29" /></th> | + | <th width="29" height="29" bgcolor="# FFFFFF" scope="row"><img src="https://static.igem.org/mediawiki/2011/0/0c/PekingR_PDF_download_logo.png" alt="" width="30" height="29" /></th> |
| - | <th width="410" nowrap="nowrap" bgcolor="# DCF8A9" class="download" scope="row"><a href="https:// static.igem.org/ mediawiki/ 2011/ 2/26/Peking_R_Notebook_from_YXW.pdf" class="clickteam">Download her note</a></th> | + | <th width="410" nowrap="nowrap" bgcolor="# FFFFFF" class="download" scope="row"><a href="https:// 2011.igem.org/ Team:Peking_R/ Notebook/ ZZR" class="clickteam">Download her note</a></th> |
| - | <th rowspan="40" bgcolor="#DCF8A9" scope="row"> </th> | + | <th rowspan="14" bgcolor="#FFFFFF" scope="row"> </th> |
| | </tr> | | </tr> |
| | <tr> | | <tr> |
| - | <th height="23" colspan="2" bgcolor="#DCF8A9" class="name" scope="row">Contents:</th> | + | <th height="23" colspan="2" bgcolor="#FFFFFF" class="name" scope="row">Contents:</th> |
| | </tr> | | </tr> |
| | <tr> | | <tr> |
| - | <th height="8" colspan="2" bgcolor="#DCF8A9" scope="row"><hr /></th> | + | <th height="8" colspan="2" bgcolor="#FFFFFF" scope="row"><hr /></th> |
| | </tr> | | </tr> |
| | <tr> | | <tr> |
| - | <th colspan="2" bgcolor="# DCF8A9" class="July"> <a href="#July" class="clickteam">*July,2011</a></th> | + | <th colspan="2" bgcolor="# FFFFFF" class="July"> <a href="#July" class="clickteam">*July,2011</a></th> |
| | </tr> | | </tr> |
| | <tr> | | <tr> |
| - | <th colspan="2" bgcolor="# DCF8A9" class="July" scope="row"><a href="#Augest" class="clickteam">*Augest,2011</a></th> | + | <th colspan="2" bgcolor="# FFFFFF" class="July" scope="row"><a href="#Augest" class="clickteam">*Augest,2011</a></th> |
| | </tr> | | </tr> |
| | <tr> | | <tr> |
| - | <th colspan="3" bgcolor="# DCF8A9" class="July" scope="row"><a href="#September" class="clickteam">*September,2011</a></th> | + | <th colspan="3" bgcolor="# FFFFFF" class="July" scope="row"><a href="#September" class="clickteam">*September,2011</a></th> |
| | </tr> | | </tr> |
| | <tr> | | <tr> |
| - | <th colspan="3" bgcolor="# DCF8A9" class="July" scope="row"><a href="#October" class="clickteam">*October,2011</a></th> | + | <th colspan="3" bgcolor="# FFFFFF" class="July" scope="row"><a href="#October" class="clickteam">*October,2011</a></th> |
| | </tr> | | </tr> |
| | <tr> | | <tr> |
| - | <th colspan="3" bgcolor="#DCF8A9" scope="row"><hr /></th> | + | <th colspan="3" bgcolor="#FFFFFF" scope="row"><hr /></th> |
| | </tr> | | </tr> |
| | <tr> | | <tr> |
| - | <th colspan="3" bgcolor="#DCF8A9" scope="row"> </th> | + | <th colspan="3" bgcolor="#FFFFFF" scope="row"><hr /> |
| - | </tr>
| + | <p class="selfstatement3"><span class="selfstatement3">7.1--7.7<a name="d701" id="d701"></a><a name="d702" id="d702"></a><a name="d703" id="d703"></a><a name="d704" id="d704"></a><a name="d705" id="d705"></a><a name="d706" id="d706"></a><a name="d707" id="d707"></a></span></p> |
| - | <tr>
| + | <hr /> |
| - | <th colspan="3" bgcolor="#DCF8A9" class="Julytitle" scope="row">July
| + | |
| - | <a name="July" id="July"></a> <hr /></th>
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| - | </tr>
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| - | <tr>
| + | |
| - | <th colspan="3" bgcolor="#DCF8A9" scope="row"><table width="500" border="0" cellspacing="0" cellpadding="0"> | + | |
| - | <tr>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Sun.</th>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Mon.</th>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Tue.</th>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Wed.</th>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Thu.</th>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Fri.</th>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Sat.</th>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="row">-</th>
| + | |
| - | <td bgcolor="#DCF8A9" class="sunday"><span class="sunday">-</span></td>
| + | |
| - | <td bgcolor="#DCF8A9" class="sunday">-</td>
| + | |
| - | <td bgcolor="#DCF8A9" class="sunday">-</td>
| + | |
| - | <td bgcolor="#DCF8A9" class="sunday">-</td>
| + | |
| - | <td valign="middle" bgcolor="#DCF8A9" class="sunday"><a href="#d701" class="clickteam">1</a></td>
| + | |
| - | <td bgcolor="#DCF8A9" class="sunday"><a href="#d702" class="clickteam">2</a></td>
| + | |
| - | </tr>
| + | |
| - | <tr class="clickteam">
| + | |
| - | <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#703" class="clickteam">3</a></span></th>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d704" class="clickteam">4</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d705" class="clickteam">5</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d706" class="clickteam">6</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d707" class="clickteam">7</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d708" class="clickteam">8</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d707" class="clickteam">9</a></span></td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d710" class="clickteam">10</a></span></th>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d711" class="clickteam">11</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d712" class="clickteam">12</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d713" class="clickteam">13</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d714" class="clickteam">14</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#715" class="clickteam">15</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d716" class="clickteam">16</a></span></td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d717" class="clickteam">17</a></span></th>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d718" class="clickteam">18</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d719" class="clickteam">19</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d720" class="clickteam">20</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d721" class="clickteam">21</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d722" class="clickteam">22</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d723" class="clickteam">23</a></span></td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d724" class="clickteam">24</a></span></th>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d725" class="clickteam">25</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d726" class="clickteam">26</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d727" class="clickteam">27</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d728" class="clickteam">28</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d729" class="clickteam">29</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d730" class="clickteam">30</a></span></td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d731" class="clickteam">31</a></span></th>
| + | |
| - | <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
| + | |
| - | </tr>
| + | |
| - | </table></th>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th height="15" colspan="3" bgcolor="#DCF8A9" class="download" scope="row"><hr /></th>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th height="16" colspan="3" bgcolor="#DCF8A9" class="download" scope="row"><a href="#topjuly" class="clickteam">[Top]</a></th>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th height="31" colspan="3" bgcolor="#DCF8A9" class="download" scope="row"> </th>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><p class="selfstatement3"><span class="selfstatement3">7.1--7.7 | + | |
| - | •Positive transformation of the parts which may be used in our project from 2011 Distribution.
| + | |
| - | <a name="d701" id="d701"></a><a name="d702" id="d702"></a><a name="d703" id="d703"></a><a name="d704" id="d704"></a><a name="d705" id="d705"></a><a name="d706" id="d706"></a><a name="d707" id="d707"></a></span></p> | + | |
| | <p> </p> | | <p> </p> |
| | <p> </p> | | <p> </p> |
| | <p> </p> | | <p> </p> |
| - | <p class="selfstatement3"><span class="selfstatement3">7.8-7.16 | + | <p class="selfstatement3"><span class="selfstatement3">7.8-7. 14<a name="d708" id="d708"></a><a name="d709" id="d709"></a><a name="d710" id="d710"></a><a name="d711" id="d711"></a><a name="d712" id="d712"></a><a name="d713" id="d713"></a><a name="d714" id="d714"></a></span></p> |
| - | •Primary construction of the part about c-di-GMP responsive ribozyme.
| + | |
| - | At first, assembly the c-di-GMP responsive ribozyme with GFP using OE-PCR (pBAD+c-di-GMP ribozyme+GFP+ter B0015). Because of the delayed primers, the problems of DNA purification kit and gel extraction kit, the ignorance of the question on fidelity of Taq enzyme, these experiments have been done many times, so they’re finished a little late. Whatever, WT、M6、M8 and GFP have been OK now.
| + | |
| - | •The growth curve determination of DH5α in media of LB.
| + | |
| - | <a name="d708" id="d708"></a><a name="d709" id="d709"></a><a name="d710" id="d710"></a><a name="d711" id="d711"></a><a name="d712" id="d712"></a><a name="d713" id="d713"></a><a name="d714" id="d714"></a><a name="d715" id="d715"></a><a name="d716" id="d716"></a></span></p> | + | |
| | <p> </p> | | <p> </p> |
| - | <p class="selfstatement3"><span class="selfstatement3">7.17-7.24 | + | <p class="selfstatement3"><span class="selfstatement3">7. 15-7. 20<a name="d715" id="d715"></ a><a name="d716" id="d716"></a><a name="d717" id="d717"></a><a name="d718" id="d718"></a><a name="d719" id="d719"></a><a name="d720" id="d720"></a></span></p> |
| - | •Assembly the c-di-GMP responsive ribozyme (WT/M6/M8) with GFP using OE-PCR successfully.
| + | |
| - | •Some comparisons between different kits.
| + | |
| - | </a><a name="d717" id="d717"></a><a name="d718" id="d718"></a><a name="d719" id="d719"></a><a name="d720" id="d720"></a></span></p> | + | |
| | <p> </p> | | <p> </p> |
| | + | <p> </p> |
| | + | <p class="selfstatement3"><span class="selfstatement3">7.21-7.28<a name="d721" id="d721"></a><a name="d722" id="d722"></a><a name="d723" id="d723"></a><a name="d724" id="d724"></a><a name="d725" id="d725"></a><a name="d726" id="d726"></a><a name="d727" id="d727"></a><a name="d728" id="d728"></a></span></p> |
| | + | <p class="selfstatement3"><span class="selfstatement3">7.29-7.31<a name="d729" id="d729"></a><a name="d730" id="d730"></a><a name="d731" id="d731"></a></span></p> |
| | <p> </p> | | <p> </p> |
| - | <p><span class="selfstatement3">7.25-7.31 | + | <hr /> |
| - | •Violacein
| + | <hr /> |
| - | Separation of individual genes in the violacein metabolic pathway ——vioA/B/D/E.
| + | <p class="selfstatement3"><span class="selfstatement3">8.1-8. 5<a name="d801" id="d801"></a><a name="d802" id="d802"></a><a name="d803" id="d803"></a><a name="d804" id="d804"></a></span><a name="d805" id="d805"></a></p> |
| - | The primers of vioC seem to have a prefer annealing ability with a particular sequence within vioC.
| + | |
| - | Testing and optimizing the protocols of PCR.
| + | |
| - | <a name="d721" id="d721"></a><a name="d722" id="d722"></a><a name="d723" id="d723"></a><a name="d724" id="d724"></a><a name="d725" id="d725"></a><a name="d726" id="d726"></a><a name="d727" id="d727"></a><a name="d728" id="d728"></a></span></p>
| + | |
| - | <p> </p>
| + | |
| - | <a name="d729" id="d729"></a><a name="d730" id="d730"></a><a name="d731" id="d731"></a></span></p>
| + | |
| - | <p> </p></th>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th colspan="3" bgcolor="#DCF8A9" class="Julytitle" scope="row">Augest<a name="Augest" id="Augest"></a></th>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th colspan="3" bgcolor="#DCF8A9" scope="row"><hr /></th>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th colspan="3" bgcolor="#DCF8A9" scope="row"><table width="500" border="0" cellspacing="0" cellpadding="0"> | + | |
| - | <tr>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Sun.</th>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Mon.</th>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Tue.</th>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Wed.</th>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Thu.</th>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Fri.</th>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Sat.</th>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="row">-</th>
| + | |
| - | <td bgcolor="#DCF8A9" class="sunday"><a href="#d801" class="clickteam">1</a></td>
| + | |
| - | <td bgcolor="#DCF8A9" class="sunday"><a href="#d802" class="clickteam">2</a></td>
| + | |
| - | <td bgcolor="#DCF8A9" class="sunday"><a href="#d803" class="clickteam">3</a></td>
| + | |
| - | <td bgcolor="#DCF8A9" class="sunday"><a href="#d804" class="clickteam">4</a></td>
| + | |
| - | <td valign="middle" bgcolor="#DCF8A9" class="sunday"><a href="#d805" class="clickteam">5</a></td>
| + | |
| - | <td bgcolor="#DCF8A9" class="sunday"><a href="#d806" class="clickteam">6</a></td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th align="center" bgcolor="#DCF8A9" scope="row"><a href="#d807" class="clickteam" >7</a></th>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><a href="#d808" class="clickteam">8</a></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><a href="#d809" class="clickteam">9</a></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><a href="#d810" class="clickteam">10</a></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><a href="#d811" class="clickteam">11</a></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><a href="#d812" class="clickteam">12</a></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d813" class="clickteam">13</a></span></td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d814"class="clickteam" >14</a></span></th>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d815" class="clickteam">15</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d816" class="clickteam">16</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d817"class="clickteam">17</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d818"class="clickteam">18</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d819"class="clickteam">19</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><a href="#d820"class="clickteam">20</a></td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th align="center" bgcolor="#DCF8A9" scope="row"><a href="#d821"class="clickteam">21</a></th>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><a href="#d822"class="clickteam">22</a></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><a href="#d823"class="clickteam">23</a></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d824"class="clickteam">24</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d825"class="clickteam">25</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d826"class="clickteam">26</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d827"class="clickteam">27</a></span></td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d828"class="clickteam">28</a></span></th>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d829"class="clickteam">29</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><a href="#d830"class="clickteam">30</a></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><a href="#d831"class="clickteam">31</a></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9">-</td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9">-</td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9">-</td>
| + | |
| - | </tr>
| + | |
| - | </table></th>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><hr /></th>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><span class="download"><a href="#topaugest" class="clickteam">[Top]</a></span></th> | + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"> </th>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><p class="selfstatement3"><span class="selfstatement3">8.1-8. 14 | + | |
| - | •c-di-GMP ribozyme construction.
| + | |
| - | Because the GFP fused with c-di-GMP responsive ribozyme (WT) couldn’t be ligated with pBAD and terminator B0015 for some reason. I have tried another approach——Gibson assembly and finally the sequence has been correct.
| + | |
| - | | + | |
| - | <a name="d801" id="d801"></a><a name="d802" id="d802"></a><a name="d803" id="d803"></a><a name="d804" id="d804"></a></span><a name="d805" id="d805"></a></p> | + | |
| | <p> </p> | | <p> </p> |
| - | <p class="selfstatement3"><span class="selfstatement3">8.15-9.5 | + | <p class="selfstatement3"><span class="selfstatement3">8. 6- 8. 13<a name="d806" id="d806"></a><a name="d807" id="d807"></a><a name="d808" id="d808"></a><a name="d809" id="d809"></a><a name="d810" id="d810"></a><a name="d811" id="d811"></a></span><a name="d812" id="d812"></a><a name="d813" id="d813"></a></p> |
| - | •With the help of GONG Yan, we construct c-di-GMP responsive ribozyme (M6/M8) regulatory system (pBAD+c-di-GMP M6/M8+GFP+ter B0015) using Gibson assembly.
| + | |
| - | •Construction of a new theophylline responsive ribozyme regulatory system.(pBAD+RBS+theo Parental/theo Th2P6/theo B11+GFP+ter)
| + | |
| - | NOTE:
| + | |
| - | Theo Th2P6 is a reconstructed Group I intron with an anti-theophylline aptamer.
| + | |
| - | Theo Parental is the original Group I intron.
| + | |
| - | Theo B11 is another reconstructed Group I intron which shows no activity in vivo.
| + | |
| - | | + | |
| - | | + | |
| - | <a name="d806" id="d806"></a><a name="d807" id="d807"></a><a name="d808" id="d808"></a><a name="d809" id="d809"></a><a name="d810" id="d810"></a><a name="d811" id="d811"></a></span><a name="d812" id="d812"></a><a name="d813" id="d813"></a></p> | + | |
| | <p> </p> | | <p> </p> |
| | <p class="selfstatement3"> </p> | | <p class="selfstatement3"> </p> |
| - | <a name="d814" id="d814"></a><a name="d815" id="d815"></a><a name="d816" id="d816"></a><a name="d817" id="d817"></a><a name="d818" id="d818"></a></span></p> | + | <p class="selfstatement3"><span class="selfstatement3">8.14-8.18<a name="d814" id="d814"></a><a name="d815" id="d815"></a><a name="d816" id="d816"></a><a name="d817" id="d817"></a><a name="d818" id="d818"></a></span></p> |
| - | <a name="d819" id="d819"></a><a name="d820" id="d820"></a><a name="d821" id="d821"></a><a name="d822" id="d822"></a><a name="d823" id="d823"></a><a name="d824" id="d824"></a><a name="d825" id="d825"></a></span></p> | + | <p class="selfstatement3"><span class="selfstatement3">8.19-8.25<a name="d819" id="d819"></a><a name="d820" id="d820"></a><a name="d821" id="d821"></a><a name="d822" id="d822"></a><a name="d823" id="d823"></a><a name="d824" id="d824"></a><a name="d825" id="d825"></a></span></p> |
| - | <a name="d826" id="d826"></a><a name="d827" id="d827"></a><a name="d828" id="d828"></a><a name="d829" id="d829"></a><a name="d830" id="d830"></a><a name="d831" id="d831"></a></span></p> | + | <p class="selfstatement3"><span class="selfstatement3">8.26-8.31<a name="d826" id="d826"></a><a name="d827" id="d827"></a><a name="d828" id="d828"></a><a name="d829" id="d829"></a><a name="d830" id="d830"></a><a name="d831" id="d831"></a></span></p> |
| | <p> </p> | | <p> </p> |
| | <p> </p> | | <p> </p> |
| Line 381: |
Line 199: |
| | <p> </p> | | <p> </p> |
| | <p> </p> | | <p> </p> |
| - | <p> </p></th> | + | <p> </p> <hr /> |
| - | </tr>
| + | <p class="selfstatement3"><span class="selfstatement3">9.1- 9. 8<a name="d901" id="d901"></a><a name="d902" id="d902"></a><a name="d903" id="d903"></a><a name="d904" id="d904"></a><a name="d905" id="d905"></a><a name="d906" id="d906"></a><a name="d907" id="d907"></a><a name="d908" id="d908"></a></span></p> |
| - | <tr>
| + | <p class="selfstatement3"><span class="selfstatement3">9.9-9.15<a name="d909" id="d909"></a><a name="d910" id="d910"></a><a name="d911" id="d911"></a><a name="d912" id="d912"></a><a name="d913" id="d913"></a><a name="d914" id="d914"></a><a name="d915" id="d915"></a></span></p> |
| - | <th colspan="3" bgcolor="#DCF8A9" class="Julytitle" scope="row">September<a name="September" id="September"></a></th>
| + | <p class="selfstatement3"><span class="selfstatement3">9.16-9.22<a name="d916" id="d916"></a><a name="d917" id="d917"></a><a name="d918" id="d918"></a><a name="d919" id="d919"></a><a name="d920" id="d920"></a><a name="d921" id="d921"></a><a name="d922" id="d922"></a></span></p> |
| - | </tr>
| + | <p class="selfstatement3"><span class="selfstatement3">9.23-9.30<a name="d923" id="d923"></a><a name="d924" id="d924"></a><a name="d925" id="d925"></a><a name="d926" id="d926"></a><a name="d927" id="d927"></a><a name="d928" id="d928"></a><a name="d929" id="d929"></a><a name="d930" id="d930"></a></span></p> |
| - | <tr>
| + | |
| - | <th colspan="3" bgcolor="#DCF8A9" class="Julytitle" scope="row"><hr />
| + | |
| - | </th>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th height="71" colspan="3" bgcolor="#DCF8A9" scope="row"><table width="500" border="0" cellspacing="0" cellpadding="0"> | + | |
| - | <tr>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Sun.</th>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Mon.</th>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Tue.</th>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Wed.</th>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Thu.</th>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Fri.</th>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Sat.</th>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="row">-</th>
| + | |
| - | <td bgcolor="#DCF8A9" class="sunday">-</td>
| + | |
| - | <td bgcolor="#DCF8A9" class="sunday">-</td>
| + | |
| - | <td bgcolor="#DCF8A9" class="sunday">-</td>
| + | |
| - | <td bgcolor="#DCF8A9" class="sunday"><a href="#d901"class="clickteam">1</a></td>
| + | |
| - | <td valign="middle" bgcolor="#DCF8A9" class="sunday"><a href="#d902"class="clickteam">2</a></td>
| + | |
| - | <td bgcolor="#DCF8A9" class="sunday"><a href="#d903"class="clickteam">3</a></td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th align="center" bgcolor="#DCF8A9" scope="row"><a href="#d904"class="clickteam">4</a></th>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><a href="#d905"class="clickteam">5</a></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><a href="#d906"class="clickteam">6</a></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><a href="#d907"class="clickteam">7</a></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><a href="#d908"class="clickteam">8</a></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><a href="#d909"class="clickteam">9</a></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d910"class="clickteam">10</a></span></td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d911"class="clickteam">11</a></span></th>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d912"class="clickteam">12</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d913"class="clickteam">13</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d914"class="clickteam">14</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d915"class="clickteam">15</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d916"class="clickteam">16</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><a href="#d917"class="clickteam">17</a></td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th align="center" bgcolor="#DCF8A9" scope="row"><a href="#d918"class="clickteam">18</a></th>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><a href="#d919"class="clickteam">19</a></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><a href="#d920"class="clickteam">20</a></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d921"class="clickteam">21</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d922"class="clickteam">22</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d923"class="clickteam">23</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d924"class="clickteam">24</a></span></td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d925"class="clickteam">25</a></span></th>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d926"class="clickteam">26</a></span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><a href="#d927"class="clickteam">27</a></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><a href="#d928"class="clickteam">28</a></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><a href="#d929"class="clickteam">29</a></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><a href="#d930"class="clickteam">30</a></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9">-</td>
| + | |
| - | </tr>
| + | |
| - | </table></th>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><hr /></th>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><span class="download"><a href="#topseptember" class="clickteam">[Top]</a></span></th>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"> </th>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><p class="selfstatement3"><span class="selfstatement3">9. 6-10.4 | + | |
| - | •Help GONG Yan constructing some parts of bistable switch. (inserting theo N8-3/1G1 into both BS1C3 and BS1C3 with 1.20/2.5)
| + | |
| - | •Constructing the part of vioC (ter B0015+pBAD+theophylline riboswitch N8-3/1G1+vioC+ter B0015).
| + | |
| - | Because the product of vioC isn’t sufficient for digesting of restriction enzyme, I insert the vioC into pUC18 first. However, it didn’t work. Finally, I tried another approach----Gibson assembly.
| + | |
| - | •Characterization of c-di-GMP ribozyme and engineered theophylline ribozyme. Collecting experiment datas.
| + | |
| - | | + | |
| - | <a name="d901" id="d901"></a><a name="d902" id="d902"></a><a name="d903" id="d903"></a><a name="d904" id="d904"></a><a name="d905" id="d905"></a><a name="d906" id="d906"></a><a name="d907" id="d907"></a><a name="d908" id="d908"></a></span></p> | + | |
| - | <a name="d909" id="d909"></a><a name="d910" id="d910"></a><a name="d911" id="d911"></a><a name="d912" id="d912"></a><a name="d913" id="d913"></a><a name="d914" id="d914"></a><a name="d915" id="d915"></a></span></p> | + | |
| - | <a name="d916" id="d916"></a><a name="d917" id="d917"></a><a name="d918" id="d918"></a><a name="d919" id="d919"></a><a name="d920" id="d920"></a><a name="d921" id="d921"></a><a name="d922" id="d922"></a></span></p> | + | |
| - | <a name="d923" id="d923"></a><a name="d924" id="d924"></a><a name="d925" id="d925"></a><a name="d926" id="d926"></a><a name="d927" id="d927"></a><a name="d928" id="d928"></a><a name="d929" id="d929"></a><a name="d930" id="d930"></a></span></p> | + | |
| | <p> </p> | | <p> </p> |
| | <p> </p> | | <p> </p> |
| Line 474: |
Line 210: |
| | </tr> | | </tr> |
| | <tr> | | <tr> |
| - | <th colspan="3" bgcolor="#DCF8A9" class="Julytitle" scope="row">October<a name="October" id="October"></a></th> | + | <th colspan="3" align=" left" bgcolor="# FFFFFF" scope="row">< p><span class=" selfstatement3"><span class=" selfstatement3">10 .1- 10.4</span><a name="d1001" id="d1001"></a><a name="d1002" id="d1002"></a><a name="d1003" id="d1003"></a><a name="d1004" id="d1004"></a></span></p> |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th colspan="3" bgcolor="#DCF8A9" scope="row"><hr /></th>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th colspan="3" bgcolor="#DCF8A9" scope="row"><table width="500" border="0" cellspacing="0" cellpadding="0">
| + | |
| - | <tr>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Sun.</th>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Mon.</th>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Tue.</th>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Wed.</th>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Thu.</th>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Fri.</th>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="col">Sat.</th>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th bgcolor="#DCF8A9" class="sunday" scope="row">-</th>
| + | |
| - | <td bgcolor="#DCF8A9" class="sunday">-</td>
| + | |
| - | <td bgcolor="#DCF8A9" class="sunday">-</td>
| + | |
| - | <td bgcolor="#DCF8A9" class="sunday">-</td>
| + | |
| - | <td bgcolor="#DCF8A9" class="sunday">-</td>
| + | |
| - | <td valign="middle" bgcolor="#DCF8A9" class="sunday">-</td>
| + | |
| - | <td bgcolor="#DCF8A9" class="sunday"><a href="#d1001" class="clickteam"><span class="clickteam">1</span></a></td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th align="center" bgcolor="#DCF8A9" scope="row"><a href="#d1002" class="clickteam">2</a></th>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><a href="#d1003" class="clickteam">3</a></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><a href="#d1004" class="clickteam">4</a></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday">5</span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday">6</span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday">7</span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday">8</span></td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th align="center" bgcolor="#DCF8A9" ><span class="sunday">9</span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday">10</span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday">11</span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday">12</span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday">13</span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday">14</span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday">15</span></td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th align="center" bgcolor="#DCF8A9" ><span class="sunday">16</span></th>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday">17</span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday">18</span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday">19</span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday">20</span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday">21</span></td>
| + | |
| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday">22</span></td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <th align="center" bgcolor="#DCF8A9" ><span class="sunday">23</span></th>
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| - | <td align="center" bgcolor="#DCF8A9"><span class="sunday">24</span></td>
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| - | <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><span class="download"><a href="#topoctober" class="clickteam">[Top]</a></span></th>
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| - | <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"> </th>
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| - | <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><p><span class="selfstatement3"></span><a name="d1001" id="d1001"></a><a name="d1002" id="d1002"></a><a name="d1003" id="d1003"></a><a name="d1004" id="d1004"></a></span></p> | + | |
| | <p> </p> | | <p> </p> |
| | <p> </p></th> | | <p> </p></th> |
| | </tr> | | </tr> |
| | <tr> | | <tr> |
| - | <th colspan="3" bgcolor="#DCF8A9" scope="row"> </th> | + | <th colspan="3" bgcolor="#FFFFFF" scope="row"> </th> |
| | </tr> | | </tr> |
| | <tr> | | <tr> |
| - | <th colspan="3" bgcolor="# DCF8A9" class="clickteam" scope="row"><a href="https://2011.igem.org/Team:Peking_R/Team/YXW" class="clickteam">==click here to her page==</a></th> | + | <th colspan="3" bgcolor="# FFFFFF" class="clickteam" scope="row"><a href="https://2011.igem.org/Team:Peking_R/Team/YXW" class="clickteam">==click here to her page==</a></th> |
| | </tr> | | </tr> |
| | <tr> | | <tr> |
| - | <th colspan="3" bgcolor="# DCF8A9" class="clickteam" scope="row"><a href="https://2011.igem.org/Team:Peking_R/Notebook">==click here to return==</a></th> | + | <th colspan="3" bgcolor="# FFFFFF" class="clickteam" scope="row"><a href="https://2011.igem.org/Team:Peking_R/Notebook">==click here to return==</a></th> |
| | </tr> | | </tr> |
| | <tr> | | <tr> |
| - | <th colspan="3" bgcolor="#DCF8A9" scope="row"> </th> | + | <th colspan="3" bgcolor="#FFFFFF" scope="row"> </th> |
| | </tr> | | </tr> |
| | </table> | | </table> |
| Line 581: |
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| | <th width="146" class="clickteam" scope="col"><span class="clickteam"><a href="https://2011.igem.org/Team:Peking_R/Notebook" class="clickteam">Personal Note</a></span></th> | | <th width="146" class="clickteam" scope="col"><span class="clickteam"><a href="https://2011.igem.org/Team:Peking_R/Notebook" class="clickteam">Personal Note</a></span></th> |
| | <th width="37" class="clickteam" scope="col">>></th> | | <th width="37" class="clickteam" scope="col">>></th> |
| - | <th width="250" align="left" class="clickteam" scope="col"><a href="https://2011.igem.org/Team:Peking_R/Notebook/YXW" class="clickteam"> Xiaowei Yan</a><a name="topjuly" id="topjuly"></a><a name="topaugest" id="topaugest"></a><a name="topseptember" id="topseptember"></a><a name="topoctober" id="topoctober"></a></th> | + | <th width="250" align="left" class="clickteam" scope="col"><a href="https://2011.igem.org/Team:Peking_R/Notebook/YXW" class="clickteam">Yan Xiaowei</a><a name="topjuly" id="topjuly"></a><a name="topaugest" id="topaugest"></a><a name="topseptember" id="topseptember"></a><a name="topoctober" id="topoctober"></a></th> |
| | </tr> | | </tr> |
| | </table> | | </table> |
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