Team:XMU-China/Result
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==population-control devices with RBS of different strength== | ==population-control devices with RBS of different strength== | ||
- | [[Image: | + | [[Image:XMU_China_114.jpg|left|Figure 2 Experimentally measured densities of BL21’s cells without population-control circuit and BL21’s cells with four population-control circuits respectively at steady state|frame|Figure 2 Experimentally measured densities of BL21’s cells without population-control circuit and BL21’s cells with four population-control circuits respectively at steady state.]] |
- | [[Image: | + | [[Image:XMU_China_115.jpg|left|Figure 3 Average of experimentally measured cell densities of BL21’s cells with four population-control circuit at steady state|frame|Figure 3 Average of experimentally measured cell densities of BL21’s cells with four population-control circuit at steady state.]] |
- | [[Image: | + | [[Image:XMU_China_116.jpg|left|Table 1 Data of the steady-state cell density|frame|Table 1 Data of the steady-state cell density.]] |
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<img src="http://partsregistry.org/wiki/images/4/41/XMU_China_block.jpg"> | <img src="http://partsregistry.org/wiki/images/4/41/XMU_China_block.jpg"> | ||
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- | Figure | + | Figure 2, figure 3 and table 1 illustrate that by using RBS of different strength in the population-control device, we were able to control the steady-state cell density of a bacteria population at different levels. And a population-control device with RBS of high strength results in a low steady-state cell density. It might be explained by the mechanism that for circuit-regulated growth, the cell death rate is regarded proportional to the intracellular concentration of the killer protein. The expression of the killer gene is regulated by the strength of its upstream RBS. Therefore, a RBS with higher strength promises more killer protein in vivo, which leads to a higher death rate of the bacteria population. |
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==population-control devices with mutation at position 3,5 and 3/5== | ==population-control devices with mutation at position 3,5 and 3/5== |
Revision as of 23:01, 5 October 2011