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Team:IIT Madras/Notebook/Protocols
From 2011.igem.org
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<div style="position:relative; left:16px;"> | <div style="position:relative; left:16px;"> | ||
<div id="protocol1">Preperation of competent DH5alpha cells</div> | <div id="protocol1">Preperation of competent DH5alpha cells</div> | ||
| - | <div id="protocol1_content"> | + | <div id="protocol1_content" style="display:none;"> |
<ul> | <ul> | ||
<li>Autoclave 0.1 M CaCl2 in 20% glycerol.</li> | <li>Autoclave 0.1 M CaCl2 in 20% glycerol.</li> | ||
Revision as of 20:23, 5 October 2011
Protocols
Preperation of competent DH5alpha cells
Transformation
Miniprep using alkaline lysis buffers
Agarose Gel Electrohoresis
Restriction digestion
Gel Elusion
PCR
PCR_purification
Ligation
