Team:Kyoto/Material

From 2011.igem.org

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==Strains(Streptomyces)==
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{| class="materials"
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!Name||Genotype and/or Relevant Features||Source or Reference
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|-
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| JM109
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| <i>end</i>A, <i>rec</i>A1, <i>gyr</i>A96,<i>thi</i>,<i>hsd</i>R17,(r<sub>κ</sub><sup>-</sup>,m<sub>κ</sub><sup>+</sup>),<i>rel</i>A1, <i>sup</i>E44,λ<sup>-</sup>,&Delta;(<i>lac-pro</i>AB),[F',<i>tra</i>D36,<i>pro</i>AB,<i>lac</i>l<sup>q</sup><i>Z</i>&Delta;M15]
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|Promega[http://www.promega.com/products/cloning-and-dna-markers/cloning-tools-and-competent-cells/bacterial-strains-and-competent-cells/bacterial-strain-jm109_de3_/]
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|-
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| BW25113
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| ∆(araD-araB)567, ∆lacZ4787(::rrnB-3), lambda-, rph-1, ∆(rhaD-rhaB)568, hsdR514
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| E.coliwiki[http://ecoliwiki.net/colipedia/index.php/BW25113]
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==Primers for PCR amplification==
==Primers for PCR amplification==
{| class="materials"
{| class="materials"

Revision as of 19:55, 5 October 2011

Contents

Materials

Strain(Fruits Fly)

NameGenotype and/or Relevant FeaturesSource or Reference
D.melanogaster(fly)Oregon-R-CDGRC[http://kyotofly.kit.jp/cgi-bin/stocks/search_res_det.cgi?DB_NUM=1&DG_NUM=105669]


Strains(E.Coli)

NameGenotype and/or Relevant FeaturesSource or Reference
JM109 endA, recA1, gyrA96,thi,hsdR17,(rκ-,mκ+),relA1, supE44,λ-,Δ(lac-proAB),[F',traD36,proAB,laclqZΔM15] Promega[http://www.promega.com/products/cloning-and-dna-markers/cloning-tools-and-competent-cells/bacterial-strains-and-competent-cells/bacterial-strain-jm109_de3_/]
BW25113 ∆(araD-araB)567, ∆lacZ4787(::rrnB-3), lambda-, rph-1, ∆(rhaD-rhaB)568, hsdR514 E.coliwiki[http://ecoliwiki.net/colipedia/index.php/BW25113]


Strains(Streptomyces)

NameGenotype and/or Relevant FeaturesSource or Reference
JM109 endA, recA1, gyrA96,thi,hsdR17,(rκ-,mκ+),relA1, supE44,λ-,Δ(lac-proAB),[F',traD36,proAB,laclqZΔM15] Promega[http://www.promega.com/products/cloning-and-dna-markers/cloning-tools-and-competent-cells/bacterial-strains-and-competent-cells/bacterial-strain-jm109_de3_/]
BW25113 ∆(araD-araB)567, ∆lacZ4787(::rrnB-3), lambda-, rph-1, ∆(rhaD-rhaB)568, hsdR514 E.coliwiki[http://ecoliwiki.net/colipedia/index.php/BW25113]


Primers for PCR amplification

DateNameSequence
07/21S Forward (Same as SRRz Forward)gGAATTCGCGGCCGCTTCTAGAGgcctggatttgttctatcagtaatcga
07/21S ReverseCTGCAGCGGCCGCTACTAGTAttattgatttctaccatcttctactccggc
07/21SRRz ReverseCTGCAGCGGCCGCTACTAGTAtgagttgcccatcgatatgggc
07/28ΔTMD1 Forwardcgcggcagatataatggcggt
08/11ΔTMD1 Reversegtcatgtttttctggcatcttcatgtct
08/11glnL Forwardtctagaggagactgctttatggcaac
08/11glnL Reverseactagtaggaactatcgtcatcgactac
08/11glnG Forwardtctagaggtgacgtttatgcaacga
08/11glnG Reverseactagtacacacaagctgtgaatcactc
08/19, 08/24Sam7 Forwardggttcattcgtgaccttctcgacttc
08/19, 08/24Sam7 Reverseaggcgataatggcgcacatc
08/20SRRz Forward RetrygGAATTCGCGGCCGCTTCTAGAGgagcaaatccccttattgggggtaagacatg
08/20SRRz Reverse RetryCTGCAGCGGCCGCTACTAGTAgcaactctatctgcactgctcattaatatacttctgg
08/23, 08/24Deletion of Upstream Forwardgagcaaatccccttattgggggtaagacatg
08/23, 08/24Deletion of Upstream Reversectctagaagcggccgcgaattcc
08/23c-glnL Forwardgctctagaggagactgctttatggcaac
08/23c-glnL Reverseggactagtaggaactatcgtcatcgactac
08/23c-glnG Forwardgctctagaggtgacgtttatgcaacga
08/23c-glnG Reverseggactagtacacacaagctgtgaatcactc
08/26gln-promoter1cggaattcgcggccgcttctagagattgca
08/26gln-promoter2rgaatgttgcaccaatatagtgcttcaatggaaacattaagcaccatgttggtgcaatctctagaagcggc
08/26gln-promoter3gaatgttgcaccaatatagtgcttcaatggaaacattaagcaccatgttggtgcaatctctagaagcggc
08/26gln-promoter4rggactagtaaaagcgaaatctgtgccaacttttaaattgcccctaaaaggcgttatcatgcgcacc
08/28cc-glnL Forwardggaattcgcggccgcttctagaggagactgctttatggcaac
08/28cc-glnG Forwardggaattcgcggccgcttctagaggtgacgtttatgcaacga
08/28rpoN Forwardggaattcgcggccgcttctagaggttctgaacatgaagcaa
08/28rpoN Reverseggactagtatccttatcggttgggtca
08/31rpoN-Pstl-m2ggactagtagtagacgctgaccccgtag
09/06rpoN-Pstl-m1gcaagatgccaaatggttgatcaagagtc
09/12ChiA1 Forwardggaattcgcggccgcttctagagattggtctggaccagtttgta
09/12ChiA1 Reverseggactagtacactgccagttccacctacc
09/12serine proteae Forwardggaattcgcggccgcttctagagcctttgtggaggaacgaaac
09/12serine proteae Reverseggactagtagtagacgctgaccccgtag
09/13, 09/22Add J23105 ForwardCAGTCCTAGGTACTATGCTAGCtactagtagcggccgctgc
09/13, 09/22Add J23105 ReverseAGCTAGCCGTAAActctagtatataaacgcagaaaggcccacc
10/10Deletion of GFP Forward (Primer1)ccaggcatcaaataaaacgaaaggctc
10/10Deletion of GFP-DT Forward (Primer2)tactagtagcggccgctgc
10/10Deletion of GFP, GFP-DT Reverse (Primer3)ctctagtattattgatttctaccatcttctactcc
- VF2 (<partinfo>G00100</partinfo>)tgccacctgacgtctaagaa
- VF (<partinfo>G00101</partinfo>)attaccgcctttgagtgagc


Primers for Real time PCR

DateNameSequence
09/13sigma54-fTTGATCCGGTCGGTGTG
09/13sigma54-rAGTGCGGAAGTCGTGATTG
09/13GAPDH-fGGTGCGAAGAAAGTGGTTATG
09/13GAPDH-rTGGTGCAGGAAGCGTTG
09/13GUS-fGGCAACAAGCCGAAAGAAC
09/13GUS-rGGCAATACTCCACATCACCAC
09/13TBP-fCAATTCATCTCGACCATCACAC
09/13TBP-rAAATCCACCACCTCTTTGACC
09/13PGK-fTCAACAAAGGCGAGAAGAAAG
09/13actin-fACGGTGTGGTTTACTCCTCTTC
09/13PGK-rGAACCGATTTCGTGCTTGATAC
09/23GUS-fACAGCCAAAAGCCAGACAG
09/23GUS-rTGACGACCAAAGCCAGTAAAG
09/23TBP-fACGGCTCATGGGATGTATG
09/23TBP-rATCCCCAGTTTGATGGTTTG
09/23glnL1-fGAGATGGCTCCGATGGATAAC
09/23glnL1-rACTAAATCACGGGCAGCAAC
09/23glnL2-fTGCTGCCCGTGATTTAGTG
09/23glnL2-rATTCGAGTAGTGATGGGTCAGG
09/23glnL3-fGATACGCTGTTTTACCCGATG
09/23glnL3-rGATAGGCAGGTAAACCGAGAAC
09/23glnG1-fGGCGGTACATTATTCCTCGAC
09/23glnG1-rTCCGCACATCCACTTTCAC
09/23glnG2-fTCAGCGCCTATCAACAAGG
09/23glnG2-rTGCTGCTGTTCCTGGTAATG
09/23glnG3-fCAGATTAAACAGCGCCATCC
09/23glnG3-rCAAACGCCCCTTGTTGATAG
09/23sigma54promoter-rpoN-factaGAGGTTCTGaacatgaagc
09/23sigma54promoter-rpoN-rattactctccagcgcctgc
09/23rpoN-glnLG-ftactagaGGAGACTGCTTTatggc
09/23rpoN-glnLG-rcgccaggttgtcatcgattaac
09/28bolA-fCGTTTTCTGAATCGTCATCG
09/28bolA-fCGTTTTCTGAATCGTCATCG
09/28bolA-rACAGGGAGGAGAGGCAAAG
09/28csiE1-fGCTCCACCATCTGTCCTTTC
09/28csiE1-rGGCAATATCATCATCCACACC
09/28csi2-rATTGTCTGTCCCGCTGATG
09/28dps-fGGGACCACTCAAGTTATCAACAG
09/28dps-rGGTCAGCCAGTTCTTTCAGG
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