Team:NYMU-Taipei/results/immunological-solution1

From 2011.igem.org

(Difference between revisions)

Revision as of 18:47, 5 October 2011 (view source) Avancy (Talk | contribs) ← Older edit		Revision as of 18:55, 5 October 2011 (view source) Nymuchang (Talk | contribs) Newer edit →
Line 18:		Line 18:
-	<font size=3>These are measurements of construct tetR+RBS+GFP under fluorescence microscope. The left graph is control group of E.coli strain DH5a. The middle is tetR+RBS+GFP in plasmid c3 in E.coli , while the right is plate without any bacilli. All graphs are measured in fixed exposure time.   Construct of tetR+RBS+GFP which emits light is thought to be a leak phenomenon of plasmid, for RE digest has proved the sequence length right, and further sequencing work is under operation.	+	<font size=3>These are the measurements of our construct tetR+RBS+GFP under fluorescence microscope. The left graph is from the control group of ''E. coli'' strain DH5a. The middle is tetR+RBS+GFP in plasmid c3 in ''E. coli'', while the right is from a plate without any bacilli. All graphs are measured in fixed exposure time. Construct of tetR+RBS+GFP which emits light is thought to be a leak phenomenon of plasmid, for RE digest has proven the sequence length is correct, and further sequencing work is under operation.
-	'''GFP measurement under different exxcitation wavelength'''	+	'''GFP measurements under different exxcitation wavelengths'''
	[[Image:33.JPG]]		[[Image:33.JPG]]
-	<font size=3>The expression of GFP is measured precisely under excitation wavelength from 475nm to 501nm, given the absorption wavelength 511nm.  In our measurement, wavelength over 493nm yields high background, resulting in values of control group surging far beyond normal condition.  Thus, 493nm is suggested as the best excitation wavelength, as it presents the highest emission light and the lowest of control group.	+	<font size=3>The expression of GFP is measured precisely under excitation wavelength from 475nm to 501nm, given the absorption wavelength 511nm.  In our measurement, wavelength over 493nm yields high background, resulting in values of control group surging far beyond normal condition.  Thus, 493nm is suggested as the best excitation wavelength, as it presents the highest emission light and the lowest of the control group.

---

Revision as of 18:55, 5 October 2011

• Home Get to HOME
• Idea Our Idea
  Concept Brainstorming Background Review
• Design/ Experiment
  Optomagnetic Design Protein Modelling NeuroSymbiosis Chassis SynthoPrime Lab Protocol
• Results
  Optomagnetic Bacteria NeuroSymbiosis Chassis Parts International Collaboration
• Human Practice & Safety
  Synthetic Neurobiology Human Practice Evaluation & Safety Assessment
• About Us
  The School The Team China Meetup Acknowledgement

tetR+RBS+GFP

measurements of construct tetR+RBS+GFP under fluorescence microscope

These are the measurements of our construct tetR+RBS+GFP under fluorescence microscope. The left graph is from the control group of E. coli strain DH5a. The middle is tetR+RBS+GFP in plasmid c3 in E. coli, while the right is from a plate without any bacilli. All graphs are measured in fixed exposure time. Construct of tetR+RBS+GFP which emits light is thought to be a leak phenomenon of plasmid, for RE digest has proven the sequence length is correct, and further sequencing work is under operation.

GFP measurements under different exxcitation wavelengths

The expression of GFP is measured precisely under excitation wavelength from 475nm to 501nm, given the absorption wavelength 511nm. In our measurement, wavelength over 493nm yields high background, resulting in values of control group surging far beyond normal condition. Thus, 493nm is suggested as the best excitation wavelength, as it presents the highest emission light and the lowest of the control group.